Just lifting a nger involves the coordinated opening and closing of billions of ion channels. These natural nanotubes play an essential role in the physiology of all living creatures, from bacteria to humans [1, 2]. By providing for the exchange of ions across cellular membranes, they control a vast range of biological processes, and their dysfunction leads to numerous diseases.

study we used a selection of peptide neurotoxins against the cardiac NaV1.5 channel to demonstrate the feasibility of screening slow-binding molecules by applying sophisticated voltage protocols, and acquiring stable, high quality recording for more than 30mins. The toxins used were: 1) JingZhaotoxin-II which is a potentiator or positive modulator of NaV1.5 channels, 2) ProTx-II, which is an inhibitor of NaV1.5 channels, and 3) u-Conotoxin PIIIA a NaV1.4 inhibitor was used as a negative control. The IonWorks Barracuda Plus platform was used for these studies. Results are presented which are in good agreement with peer-reviewed publications. Taken together, these results demonstrate the high-throughput capabilities of this platform for measuring peptide toxins targeted against ion channels.