The present investigation entitled with “Polyamine effect on biochemical events in sugarcane grown on sodic soils” was carried out to study the efficacy of polyamines viz., putrescine and spermidine to ameliorate the effect of soils sodicity by analysing the lipid peroxidation, chlorophyll a content, chlorophyll b content, total chlorophyll content, reducing sugars, non-reducing sugar, and total sugars. The leaves of the two sugarcane cultivars viz., the salt tolerant CoM 0265 and salt susceptible CoC 671 grown on sodic soils and polyamines were applied at 0, 100 and 500μM concentration. The two foliar application of these polyamines were carried out 45 days after planting with 8 days interval. The leaf samples were collected after 65 days after planting for futher biochemical analysis. The effect of polyamines was comparatively higher in CoM 0265 for lipid peroxidation than the CoC 671. The proline and glycine betaine increased however, sugars were decrease in the sugarcane leaves in both the cultivars when grown in sodic soils. The application of polyamines increased the proline, glycine betaine and sugars content in these two cultivars with increase in concentration of polyamines. Interestingly the proline content was found to be higher in salt susceptible cultivar CoC 671 at 500μM polyamine application than the salt tolerant cultivars CoM 0265.

The present investigation entitled with “Polyamine effect on biochemical events in sugarcane grown on sodic soils” was carried out to study the efficacy of polyamines viz., putrescine and spermidine to ameliorate the effect of soils sodicity by assaying the osmolytes viz., proline, glycine betaine, the antioxidant enzymes viz., ascorbate peroxidase, catalase, superoxide dismutase. These were assayed from the leaves of the two sugarcane cultivars viz., the salt tolerant CoM 0265 and salt susceptible CoC 671 grown on sodic soils. The polyamines were applied at 0, 100 and 500μM concentration. The two foliar application of these polyamines were carried out 45 days after planting with 8 days interval. The leaf samples were collected after 65 days after planting for futher biochemical analysis. The antioxidant activities viz., ascorbate peroxidase, catalase and superoxide dismutase were found to be higher in salt tolerant cultivar CoM 0265 than CoC 671 a salt susceptible cultivar on normal and plants grown on sodic soils. Sodicity caused increase in activities of these enzymes in both the cultivars. However, with the application of polyamine the activities were increased more than the control in both cultivars. It was observed that the activity of ascorbate peroxidase was significantly higher at 500μM concentration of polyamines in salt susceptible cultivar CoC 671 than the salt tolerant CoM 0265 cultivar of sugarcane.

Sugarcane is (Saccharum officinarum L.) is one of the most important commercial crops of the world, including region where water availability is limited or highly inconsistent. Abiotic stresses may decline in potential yield of crop plants by 70 %. In sugarcane numerous environmental factors can have negative effects on the increase in expansion of production and salinity stress is one of the most limiting abiotic factors affecting sugarcane productivity. Two sugarcane varieties viz., MS-10001 and CoC-671 were sown in the pots and were evaluated for antioxidative enzymes activity of Ascorbate peroxidase (APX), Catalase (CAT) and Superoxide Dismutase (SOD) under different salt stress levels (0, 2, 4, 8 dSm-1). The activity of antioxidative enzymes APX was recorded highest in MS-10001 at (2, 4, 8 dSm-1) salt stresses and less per cent increase was found in APX activity in CoC-671 at different salt stress level. The activity of antioxidative enzymes Superoxide dismutase and catalase was recorded higher in MS-10001 as compared to CoC-671.

Sugarcane (Saccharum sp.) has great economic importance due to its application in the food industry and is also particularly valuable for its use in the production of ethanol and a less polluting renewable bio fuel (Menossi et al., 2008). Originating in Asia, sugarcane is highly productive in the tropical and sub-tropical areas of the world. However, the adverse environmental conditions affect the growth and development of sugarcane plants (Lisson et al., 2005 and Inman Bamber and Smith, 2005). High salt concentrations decrease the osmotic potential of soil solution creating a water stress in plants and also cause International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 12 (2019) Journal homepage: http://www.ijcmas.com

Sugarcane is cultivated throughout the Indo-Gangetic plains of South Asia and Maharashtra is second largest producer. The leaf samples were collected at different stages of crop growth from Rahuri farm and were evaluated for nitrate assimilating enzyme viz., in vitro nitrate reductase and sucrose metabolizing enzymes activities viz., sucrose synthase, sucrose phosphate synthase and acid invertase. The experiment was laid out in R.B.D. with four replications and six treatments. Nitrogen fertigation was given as 100 % N through urea (T2), 50 % N + Acetobacter diazotrophicus (T3), 25 % N + consortium endophytic bacteria (T4), 0 % N + consortium endophytic bacteria (T5), 0 % N +without consortium endophytic bacteria (T6) and absolute control (T1). The results of the experiment revealed that enzymes activities viz., in vitro nitrate reductase, sucrose synthase, sucrose phosphate synthase and acid invertase by sugarcane crop was significantly higher in T4 treatment (25 % N + foliar application of consortium of endophytic bacteria) followed by Recommended Dose of Fertilizer (R.D.F.) treatment and 0% N with foliar application of consortium of endophytic bacteria. Hence, the use of foliar spray of consortium of N fixers @ 25 % concentration can save 75 % of nitrogen without affecting yield.