The aging population and the increasing prevalence of sports-related injuries have made osteochondral defects a major global health issue. The repair of osteochondral defect is still a challenge due to poor regeneration of both cartilage and bone tissues. To address this issue, this study developed a biomimetic dual-layer scaffold based on SilMA hydrogel using DLP technology. For the upper area of scaffold, PEGDA was used to enhance the mechanical strength of SilMA hydrogel, making it more suitable for the environment of articular cartilage, while cell microspheres were incorporated as 3D carriers for chondrocyte encapsulation and delivery, providing seed cells for regeneration. For the lower area of scaffold, the lower part was loaded with hydroxyapatite to promote bone tissue regeneration. This study systematically investigated the design of 3D printable inks for osteochondral defect repair, exploring the effects of PEGDA and HAp on the printability and physicochemical properties of SilMA bioink through orthogonal experiments. In vitro and in vivo results demonstrated that the biomimetic dual-layer scaffold exhibited good biocompatibility, promoting cell adhesion and proliferation. Thus, these dual-layer scaffolds loaded with chondrocyte microspheres effectively promote the synchronous repair of both articular cartilage and subchondral bone.

Study objectivesThis study aimed to identify the risk factors associated with falls in hospitalized patients, develop a predictive risk model using machine learning algorithms, and evaluate the validity of the model's predictions.Study designA cross-sectional design was employed using data from the DRYAD public database.Research methodsThe study utilized data from the Fukushima Medical University Hospital Cohort Study, obtained from the DRYAD public database. 20% of the dataset was allocated as an independent test set, while the remaining 80% was utilized for training and validation. To address data imbalance in binary variables, the Synthetic Minority Oversampling Technique combined with Edited Nearest Neighbors (SMOTE-ENN) was applied. Univariate analysis and least absolute shrinkage and selection operator (LASSO) regression were used to analyze and screen variables. Predictive models were constructed by integrating key clinical features, and eight machine learning algorithms were evaluated to identify the most effective model. Additionally, SHAP (Shapley Additive Explanations) was used to interpret the predictive models and rank the importance of risk factors.ResultsThe final model included the following variables: Adl_standing, Adl_evacuation, Age_group, Planned_surgery, Wheelchair, History_of_falls, Hypnotic_drugs, Psychotropic_drugs, and Remote_caring_system. Among the evaluated models, the Random Forest algorithm demonstrated superior performance, achieving an AUC of 0.814 (95% CI: 0.802-0.827) in the training set, 0.781 (95% CI: 0.740-0.821) in the validation set, and 0.795 (95% CI: 0.770-0.820) in the test set.ConclusionMachine learning algorithms, particularly Random Forest, are effective in predicting fall risk among hospitalized patients. These findings can significantly enhance fall prevention strategies within healthcare settings.

Biofabrication, also known as bioprinting, has been widely used in the field of biomedicine. The three most important factors in biofabrication are 3D bioprinter, biomaterials to be printed (bioinks), and the printing object (application). This review provides a detailed introduction to the latest research progress in these aspects. In particularly, the bioinks for bioprinting require strict biocompatible requirements. Four typical materials, i.e. metal/alloys, ceramics, polymers, and their composites, were introduced in detail, and their printing process and application scenarios were summarized, respectively. There are many applications of biofabrication in clinical practice. The application of biofabrication in skeletal system, skin and soft tissue, cardiovascular system, digestive system, respiratory system, urinary system, nervous system, plastic surgery and medical aesthetics were briefly introduced. The applications of biofabrication has a wide range of clinical need. Biofabrication is an innovative technology that is expected to further promote the clinical precision treatments.

OBJECTIVES:The study aimed to compare the treatment cost, operation time, clinical effect, and complications between punctures done under magnetic resonance imaging (MRI) planning based on picture archiving and communication systems (PACS) and punctures done under immediate X-ray fluoroscopy guidance in the treatment of lumbar disc herniation by transforaminal lumbar epidural injection. PATIENTS AND METHODS:In this prospective study conducted between October 2016 and June 2021, 128 patients were randomly divided into Groups A and B by the random number table method. In Group A (n=66; 36 males, 30 females; mean age: 64.5±2.4 years, range, 50 to 72 years), puncture was performed by planning with PACS-based MRI; in Group B (n=62; 34 males, 28 females; mean age: 65.3±2.6 years; range, 48 to 73 years), puncture was performed under immediate X-ray guidance. The cost of treatment, duration of procedure, clinical outcome, and complications were compared between the two groups. RESULTS:The difference in treatment cost in Groups A and B was statistically significant (p<0.001), with 755.67±29.45 yuan and 1.158.08±43.92 yuan, respectively. The mean treatment time was statistically significant (p<0.001) between the groups, with 21.16±1.91 min in Group A and 37.26±2 min in Group B. However, there was no significant difference between Group A and Group B in terms of improvement in pain scores and Oswestry disability index (both p>0.05). There was also no significant difference between Group A and Group B in terms of complication rates (both p>0.05). CONCLUSION:Compared to immediate X-ray guided puncture, the puncture method using PACS for MRI planning shortened the transforaminal lumbar epidural injection procedure time and reduced the treatment costs without exposing the physician or patient to additional radiation, while there was no significant difference in the short-term clinical outcome or complication rate.

目的 分析外周血微小核糖核酸(miR)-92a、miR-424、miR-145在食管癌中的表达及意义.方法 选取2018年1月—2020年12月我院68例食管癌作为食管癌组,另纳入68例体检健康者作为对照组,比较2组外周血miR-92a、miR-424、miR-145相对表达量,采用受试者工作特征(ROC)曲线评估外周血miR-92a、miR-424、miR-145相对表达量对食管癌的诊断价值;分析食管癌临床病理特征与外周血miR-92a、miR-424、miR-145的相关性.结果 食管癌组外周血miR-92a、miR-424相对表达量显著高于对照组,miR-145相对表达量低于对照组(P<0.01).ROC曲线分析发现,外周血miR-92a、miR-424、miR-145相对表达量均对食管癌有较高的诊断价值.Spearman秩相关分析显示,食管癌TNM分期及淋巴结转移与外周血miR-92a、miR-424相对表达量呈正相关(r=0.692、0.511、0.496、0.404,P<0.05),与miR-145相对表达量呈负相关(r=-0.475、-0.382,P<0.05).结论 miR-92a、miR-424在食管癌中发挥致癌效应,miR-145发挥抑癌效应;外周血miR-92a、miR-424、miR-145检测有利于判断食管癌侵袭及迁移能力.

Adding gentamicin to silk fibroin enhances both the antibacterial performance and degradation rate of silk-based materials. The increased material degradation rate can affect the strength of early internal fixation, resulting in internal fixation failure. This study sought to adjust the gentamicin concentration to control the material degradation rate, thereby better meeting clinical application requirements. The in vitro degradation, water absorption rate, and expansion rate of silk-based materials containing different gentamicin concentrations were studied. A gentamicin-loaded silk-based screw was implanted into the femurs of New Zealand rabbits. Micro-computed tomography was used to measure the screw diameter, which was then used to calculate the degradation rate. The specimens were stained with hematoxylin and eosin and Masson's trichrome. The in vitro results revealed increasing material degradation rates with increasing gentamicin concentration but no significant differences in water absorption rates with different gentamicin concentrations. The degradation rates of gentamicin-loaded (4 mg/g) silk-based rod-like materials were approximately 11.08% at three months in vitro and 9.4% in the animal experiment. The time for complete degradation was predicted from the fitting curve to be approximately 16 months. No inflammatory hyperplasia was observed in bone or soft tissue. The degradation and biocompatibility of the material containing 4 mg/g gentamicin meet clinical application requirements, and previous experimental results demonstrate good antibacterial performance of materials containing this gentamicin concentration.

RATIONALE:Streptococcus gordonii is a rare cause of finger suppurative infection. Very few cases have been reported of its treatment.PATIENT CONCERNS:A 68-year-old male of severe finger infection. Bacterial culture of synovial fluid revealed S gordonii.According to the patient's history and auxiliary examination, the patient was diagnosed with S gordonii infection. Here, we review the diagnosis and treatment of this patient and describe the clinical and epidemiological characteristics of the patient.DIAGNOSES:Streptococcus gordonii finger infection.Interventions: In the case of ineffective oral antibiotics, this patient chose to pursue an abscess incision, but in the course of treatment,the flexor digitorum tendon dissolved and eventually ruptured.OUTCOMES:The infection was controlled after intravenous injection of vancomycin. The incision was sutured 2 weeks later. No recurrence of infection was found after 3 months of follow-up.LESSONS:The treatment included antibacterial and abscess treatments. In the absence of drug sensitivity results, antibiotics can be used empirically. If empirical anti-microbial treatment fails, the antibiotic regimen should be changed in a timely manner, Vancomycin may be an antibiotic choice.

肝豆状核变性(hepatolenticular degeneration,HDL)是一种由ATP7B基因突变引起的常染色体隐形疾病[1],主要发生在5～35岁的人群中,以儿童及青少年多见,中国和亚洲其他国家的患病率比西方国家高.该病可导致铜代谢障碍,主要表现为肌张力障碍、震颤等神经精神表现;急性肝炎、慢性肝病或肝硬化等肝脏损害;其他系统损害如肾脏、骨关节、心肌等方面的损害.部分患者存在严重的姿势性震颤如"扑翼样震颤",常被误诊为代谢性脑病,也有患者存在肢体僵硬和运动迟缓,易被误诊为帕金森病,出现骨关节症状特别是以骨关节症状为唯一首发症状的病例往往会被误诊为骨质疏松、骨关节炎、生长痛而延误治疗,且预后相对不良.现对本院收治的出现骨关节症状的HDL病例分析报道如下.

骨质疏松是一种常见的骨骼疾病,临床分为原发性、继发性和特发性三种.原发性骨质疏松主要表现为绝经后骨质疏松和老年性骨质疏松;继发性骨质疏松是由某些代谢性疾病或者长期服用某些药物等因素所诱发[1].在中国,60岁以上人群骨质疏松总体患病率为36％,其中男23％,女49％ [2].人体和极大多数动物的胃肠道内定植着种类和数量繁多的肠道菌群,在肠道菌群中,有一类对机体代谢极其重要的微生物就是益生菌.益生菌主要具备营养作用、提高防御能力、延缓衰老、抑肿瘤、调节骨代谢等作用.因此,益生菌对于机体健康具有重要作用,目前国内外较多研究表明益生菌能够影响骨代谢,从而导致或改善骨质疏松,但是具体作用机制尚未得到完全阐明,现对其与骨质疏松相互关系的相关机制研究进展进行综述,本次研究经过本院医学伦理委员会同意.

Objective We analyzed US, CT, and MR images plus the pathological characteristics of 12 patients with pathologically confirmed schwannomatosis to explore the relationship between imaging and pathology. Method A retrospective analysis was undertaken over a 10-year period (01/01/2000-31/12/2019) of 12 patients undergoing Imaging and pathological examination for schwannomatosis, and the relevant literature was reviewed. Results The median age at diagnosis was 39.4 years (range, 22–56 years). We included the mean diameter of 36 lesions (5.10 ± 0.84 cm; range, 0.5–9.1 cm) in the focal observation. US showed that the mass boundary was clear, and most of the masses were low echo. CT showed that tumors were low density; the plain-scan CT value was 22–35 HU and enhancement amplitude 10–30 HU. MRI showed that most of the tumors were low or medium signal on T1-weighted image (T1WI) and high signal on T2WI; enhancement could be homogeneous or inhomogeneous. We could see the cellular Antoni A and myxoid Antoni B areas of the microscopic morphology. According to the proportions of the two areas, masses could be divided into four types: I, II, III and IV. Immunohistochemical (IHC) staining showed that the expression of S-100 protein was diffusely positive (36/36). Conclusions Imaging examination in schwannomatosis is helpful for localization and characterization of focus, as well as observation of the relationship between the tumor and the surrounding tissue structure. However, the specific diagnosis should also be based on pathological manifestations, IHC results and genetic analysis. Characteristics of mass imaging correlate with pathological changes in the mass.

[目的]分析开放性手部骨折Ⅰ期内固定术后感染细菌学特点及伤口修复方法.[方法] 2016年12月-2018年8月,对18例开放性手部骨折Ⅰ期内固定术后感染患者,取出内固定装置并清创,行带蒂旋髂浅动脉腹股沟皮瓣修复缺损区.[结果] 18例伤口,11例为革兰氏阳性菌,7例为革兰氏阴性菌.经取出内固定装置后感染得到控制,6例缺损面积较大未能直接缝合,使用带蒂皮瓣修复创面愈合良好,随访1年感染无复发,骨折愈合.[结论]内固定材料细菌生物膜形成,建议早期取出内固定装置,如伤口软组织缺损面积较大,可考虑使用带蒂旋髂浅动脉腹股沟皮瓣修复.

背景:丝素蛋白材料具有良好的生物相容性、力学性能及可生物降解等优点,但在临床中其作为一种缝线固定材料应用于半月板撕裂缝合修复未见报道.目的:制备载庆大霉素的丝素蛋白材料,分析其生物相容性与抗菌性能.方法:采用三元溶液溶解脱胶后的天然蚕茧,经过透析、冻干、再溶解、成型等处理制备丝素蛋白材料,将庆大霉素溶解于丝素蛋白溶液中制备载庆大霉素的丝素蛋白材料.将丝素蛋白材料、载庆大霉素的丝素蛋白材料分别与小鼠前成软骨细胞共培养,1,3,5d后,采用MTT法检测细胞增殖;共培养24 h后,采用钙黄绿素染色检测材料的细胞毒性作用,流式细胞仪检测细胞早期凋亡情况,扫描电镜观察细胞在材料表面的黏附情况.将丝素蛋白材料、载庆大霉素的丝素蛋白材料分别与抗凝血混合,采用溶血实验检测材料的溶血率.将丝素蛋白材料、载庆大霉素的丝素蛋白材料分别与金黄色葡萄球菌、大肠杆菌共培养,进行细菌计数、扫描电镜观察与抑菌圈实验.结果 与结论:①两种材料对细胞的增殖、早期凋亡无明显影响;②两种材料无明显的细胞毒性;在两种材料表面黏附的细胞生长状态良好,排列紧密,细胞形态舒展;③两种材料具有良好的血液相容性,未引起溶血反应;④载庆大霉素的丝素蛋白材料对金黄色葡萄球菌、大肠杆菌具有明显的抑制作用,且对金黄色葡萄球菌的抑制作用更明显;⑤结果表明,载庆大霉素丝素蛋白材料具有良好的生物相容性和抗菌性.

Chitosan is a biodegradable and biocompatible natural polysaccharide that has a wide range of applications in the field of dentistry due to its functional versatility and ease of access. Recent studies find that chitosan and its derivatives can be embedded in materials for dental adhesives, barrier membranes, bone replacement, tissue regeneration, and antimicrobial agent to better manage oral diseases. In this paper, we provide a comprehensive overview on the preparation, applications, and major breakthroughs of chitosan biomaterials. Furthermore, incorporation of chitosan additives for the modification and improvement of dental materials has been discussed in depth to promote more advanced chitosan-related research in the future.

目的 探讨关节镜清理术联合关节腔注射消炎镇痛类药对疼痛症状严重的中期膝骨关节炎的治疗效果.方法 选择我院自2017年6月至2017年12月诊断为膝骨关节炎(Kellgren-Lawrence分级Ⅲ级)并接受关节镜清理术和关节腔注射消炎镇痛类药(酮咯酸氨丁三醇注射液、甲磺酸罗哌卡因、复方倍他米松注射液)治疗的50例疼痛症状严重的中期膝骨关节炎患者.记录患者入院时HSS评分、VAS评分、膝关节活动度和手术并发症发生例数,对所有患者进行定期连续性随访并记录患者术后的HSS评分、VAS评分、膝关节活动度.结果 末次随访时,45例膝关节疼痛基本消失,其余关节偶有疼痛,但是疼痛程度明显减轻.膝关节屈曲活动度由术前的70-100°改善为110～135°;3例膝关节伸直受限,其余伸直均达0°.HSS评分由术前平均79.92分改善为术后87.78分,VAS评分平均由术前5.74改善为术后2.12,连续行走距离由术前平均1154米即需要休息改善为2301米才需要休息,均与术前比较差异有统计学意义(P<0.05),49例患者感觉手术效果非常满意,仅1例患者感手术效果不甚满意.结论 关节镜清理术联合关节腔内注射消炎镇痛类药对于疼痛症状严重的中期膝骨关节炎患者具有较好的短期疗效.

Hyaluronic acid (HA) is widely distributed in the human body, and it is heavily involved in many physiological functions such as tissue hydration, wound repair, and cell migration. In recent years, HA and its derivatives have been widely used as advanced bioactive polymers for bone regeneration. Many medical products containing HA have been developed because this natural polymer has been proven to be nontoxic, noninflammatory, biodegradable, and biocompatible. Moreover, HA-based composite scaffolds have shown good potential for promoting osteogenesis and mineralization. Recently, many HA-based biomaterials have been fabricated for bone regeneration by combining with electrospinning and 3D printing technology. In this review, the polymer structures, processing, properties, and applications in bone tissue engineering are summarized. The challenges and prospects of HA polymers are also discussed.

Purpose: To observe the morphology of the transverse geniculate ligament of the knee (TGL) by magnetic resonance imaging (MRI) and to analyze the cause of the pseudotear sign of the anterior horn of the meniscus caused by the TGL. Methods: Patients who underwent MRI examination of the knee joint in the orthopaedics department of our hospital from July 2016 to August 2019 were identified. The occurrence rate, length, width, thickness, cross-sectional shape, pattern, appearance, and position relative to the anterior horn of the lateral and medial meniscus and anatomical variations were observed by multiplane and multisequence MRI. The frequency and cause of the pseudotear sign also were observed. Results: The data of 101 patients were analyzed. Among them, 60 were male, and 41 were female. The average age was 42.01 (18-75) years. The occurrence rate of the TGL was 67.3% (68/101), the average length was 38.75 +/- 3.56 mm, the median coronal diameter was 1.79 +/- 0.60 mm, the median sagittal diameter was 1.88 +/- 0.35 mm, and the cross-sectional morphology was mostly oval and round. There were 5 types of TGL connection to the anterior horn of the medial meniscus: type 1, located at the front edge; type 2, located at the upper front edge; type 3, located at the upper edge; type 4, located at the back upper edge; and type 5, was located at the back edge of the anterior horn of the medial meniscus. There was only one type of TGL insertion into the anterior horn of the lateral meniscus, located at the anterior superior edge of the anterior horn of the lateral meniscus. There were 4 cases of the pseudotear sign in the anterior horn of the meniscus, 3 in the lateral meniscus and 1 in the medial meniscus. The pseudotear sign of the anterior horn of the meniscus caused by the TGL was observed at a rate of 5.88% (4/68). Conclusions: In MRI examination of the knee, the anterior horn of the meniscus sometimes shows a pseudotear sign. According to the shape and route of the TGL on MRI and the direction and position of the pseudotear sign of the anterior horn of the meniscus, true and false tears of the anterior horn of the meniscus can be identified. Level of Evidence: Level III, diagnostic study (retrospective, noncomparative, observational case series without a consistently applied reference "gold" standard).

目的 分析骨转换标志物血清1型胶原羧基端肽(β-CTX)和血清1型前胶原氨基端前肽(PINP)在亚临床型甲状腺功能减退(简称亚甲减)合并骨质疏松症患者中的表达,并探讨其能否作为判断该类患者骨质疏松程度及脆性骨折发生风险的预测指标.方法 选取2016年7月至2019年6月收治的骨质疏松症合并亚甲减患者62例(骨松甲减组),以及同期收治的单纯骨质疏松症伴骨痛患者65例(骨松骨痛组),并纳入同年龄段健康体检者68例(正常对照组).比较3组间骨代谢标志物,如血钙、血磷、血镁、碱性磷酸酶、25羟维生素D、骨密度、T值、甲状腺激素、甲状旁腺素以及骨转换标志物血清PINP及β-CTX浓度水平.结果 骨松甲减组、骨松骨痛组及正常对照组骨代谢及骨转换标志物中血钙、血磷、血镁、碱性磷酸酶、甲状旁腺素水平差异无统计学意义(P均>0.05),而25羟维生素D、骨密度、T值、β-CTX及PINP水平差异有统计学意义(P均<0.05).结论 骨松甲减组PINP、β-CTX水平降低,为低转换型骨质疏松,不能依靠骨转换标志物作为骨松甲减组骨质疏松程度的判断指标及预测骨折发生风险.

Abstract The aim of this study was to evaluate the clinical and imaging results of transforaminal debridement with a posterior-only approach involving placement of an interbody bone graft combined with diseased vertebral fixation for the treatment of thoracic and lumbar tuberculosis (TB) with a minimum 5-year follow-up. Sixty-five patients who presented with active thoracic and lumbar TB between October 2006 and August 2013 were retrospectively analyzed: 20 were thoracic TB (group A), 17 were thoracolumbar TB (group B), and 28 were lumbar TB (group C). The patient data, operating time, blood loss, Visual Analog Scale score, Oswestry Disability Index score, correction of kyphosis, recovery of neurological function, and complications were recorded and analyzed. The patients were followed for 68.7 ± 17.8 months. The preoperative average Cobb angles of kyphosis in patients in groups A, B, and C significantly decreased from 28.2 ± 11.9°, 30.5 ± 16.9°, and 10.9 ± 8.8° before surgery to 8.0 ± 5.4°, 5.0 ± 4.1°, and –4.4 ± 1.6° (– indicates lordosis) after surgery, respectively. At the final follow-up time, the Cobb angles were 9.2 ± 6.1°, 6.8 ± 10.0°, and -3.7 ± 2.0°, respectively. The postoperative Cobb angles of kyphosis were significantly improved in all groups (P < .05). The correction loss angles were larger in groups A and B than in group C (P > .05). The operating time, blood loss, and complications were not significantly different between the groups (P > .05). Three (4.6%) patients developed unhealed TB during postoperative anti-TB treatment, and 6 patients (9.2%) with TB relapsed after healing from surgery. The posterior-only approach for the surgical treatment of thoracic and lumbar TB achieved satisfactory outcomes over long-term follow-up. The implantation of pedicle screws in diseased vertebrae reduced the range of fixation, but patients with thoracic and thoracolumbar TB should undergo fixation to at least 1 adjacent normal segment. There were some cases of recurrence after TB healed, and long-term follow-up is therefore necessary.

Scaffolds with a biomimetic hierarchy micro/nanoscale pores play an important role in bone tissue regeneration. In this study, multilevel porous calcium phosphate (CaP) bioceramic orthopedic implants were constructed to mimic the micro/nanostructural hierarchy in natural wood. The biomimetic hierarchical porous scaffolds were fabricated by combining threedimensional (3D) printing technology and hydrothermal treatment. The first-level macropores (similar to 100-600 mu m) for promoting bone tissue ingrowth were precisely designed using a set of 3D printing parameters. The second-level micro/nanoscale pores (similar to 100-10,000 nm) in the scaffolds were obtained by hydrothermal treatment to promote nutrient/metabolite transportation. Micro- and nanoscale-sized pores in the scaffolds were recognized as in situ formation of whiskers, where the shape, diameter, and length of whiskers were modulated by adjusting the components of calcium phosphate ceramics and hydrothermal treatment parameters. These biomimetic natural wood-like hierarchical structured scaffolds demonstrated unique physical and biological properties. Hydrophilicity and the protein adsorption rate were characterized in these scaffolds. In vitro studies have identified micro/nanowhisker coating as potent modulators of cellular behavior through the onset of focal adhesion formation. In addition, histological results indicate that biomimetic scaffolds with porous natural wood hierarchical pores exhibited good osteoinductive activity. In conclusion, these findings combined suggested that micro/nanowhisker coating is a critical factor to modulate cellular behavior and osteoinductive activity.

Soft tissue leiomyosarcoma (STLMS) is a major histological subtype of adult sarcoma. Although the molecular mechanisms ofLMS have been gradually revealed, no valid therapeutic targets have been identified. In this study, we performed a systematic screening to explore relapse-associated genes in STLMS, using data from The Cancer Genome Atlas-Sarcoma (TCGA-SARC). Then, we investigated the functional role of the gene with the best relapse-prediction value in STLMS by both in-vitro and in-vivo studies. Results showed that AMH and PLA2G10 were two genes with area under curve (AUC) values higher than 0.80 in ROC analysis when detecting relapse. Patients in the high AMH or PLA2G10 expression group had significantly worse relapse-free survival (RFS) compared to the respective low expression group. PLA2G10 was highly expressed in STLMS, but not in other sarcoma subtypes. PLA2G10 overexpression promoted SK-LMS-1 cell growth and G1/S transition, while PLA2G10 knockdown slowed the growth and resulted in G1 phase arrest. PLA2G10 overexpression markedly increased the expression of CDK2 and cyclin E1, but did not influence CDK4, CDK6, cyclin D1, CDK1 or cyclin A expression. PLA2G10 overexpression enhanced SK-LMS-1 cell-derived xenograft tumor growth in nude mice, while PLA2G10 inhibition slowed the growth. Mutation of two critical catalyzing amino acid residues (p.H88A and p.D89A) abrogated the capability of PLA2G10 to catalyze the production of arachidonic acid (AA), and also canceled the regulatory effects on cyclin E1 and CDK2 expression, as well as G1/S transition. In conclusion, PLA2G10 was a specific relapse-associated gene in STLMS. It facilitated the cell-cycle progression of STLMS cells at least by elevating the expression of cyclin E1 and CDK2. The hydrolytic activity was crucial for its oncogenic properties.