In recent years, Luchuan pigs in southern China have been used to produce high-quality meat by crossbreeding them with Duroc boars; however, PSE (pale, soft and exudative) meat was frequently reported in the crossbred pigs, and the underlying reason remains unknown. We excluded the possibility of the well-known causative mutations in RYR1 and PRKAG3 but identified the existence of an unfavorable allele of a splicing mutation (g.8283C>A) in PHKG1 in two Duroc boars and three Duroc × Luchuan crossbred pigs with PSE meat. An association analysis with 425 Duroc × Luchuan crossbred pigs revealed that the polymorphism of the splicing site of PHKG1 has significant association with the ultimate meat pH value (P = 0.035) and color score (P = 0.004). In addition, a strong cis-eQTL (expression QTL) signal for the expression of PHKG1 was identified in 189 Duroc × Luchuan crossbred pigs, and the splicing mutation was proven to be significantly associated with the expression of PHKG1 (P = 4.01e-11). Furthermore, RNA-sequencing data analysis confirmed that 131 CC homozygotes had only one transcript (T1), with FPKM (fragments per kilobase of transcript per million) of 35.40 ± 7.28, and 58 CA heterozygotes had two types of transcripts (T1 and T2), with FPKM of 19.63 ± 5.11 and 9.20 ± 2.39 respectively. Based on the association and eQTL analysis results, we concluded that PSE meat in Duroc × Luchuan crossbred pigs is caused by the splicing mutation in PHKG1. Our findings further support the effect of the causative mutation in PHKG1 on meat quality. The GEO accession number for the data is GSE124315.

In this study, we established and characterized a cell line derived from the kidney of black carp (Mylopharyngodon piceus), which is an important freshwater aquaculture species. The cell line was designated as MPK and subcultured for more than 70 passages in DMEM medium containing 10% fetal bovine serum (FBS) at 28°C. MPK had a modal diploid chromosome number of 48. Moreover, a transient MPK transfection efficiency was up to 18% using a green fluorescent protein plasmid by a modified electroporation. In addition, the MPK cells showed susceptibility to spring viremia of carp virus (SVCV), as demonstrated by the presence of severe cytopathic effects (CPEs) and increased viral RNA. Unexpectedly, the MPK cells expressed pluripotency-associated genes such as nanog, oct4 and vasa, indicating that these are possibly adult stem cells. Taken together, we have established a stable cell line from kidney that may potentially be utilized as an in vitro platform for genetic modifications and host-pathogen analysis in black carp.

All-optical OR operation has been demonstrated using a semiconductor optical amplifier (SOA) and delayed interferometer (DI) at 80Gb/s. The DI is based on a polarization maintaining loop mirror. Q-factor of the operation is discussed through numerical simulations. The results show the OR gate operation rate is limited by the gain recovery time and input pulse energy.

An all-optical logic AND gate is demonstrated by using a semiconductor optical amplifier (SOA) based Mach-Zehnder interferometer (MZI). The AND results are numerically analyzed by solving the rate equation of SOA. Q-factor values have been calculated. The operation of the AND logic gate is experimentally demonstrated at 80Gb/s. Operation at higher data rates is feasible using SOAs with shorter phase recovery time.

The performances of all-optical logic gates AND, OR, XOR, NOT based on quantum dot semiconductor optical amplifier (QD SOA) devices have been simulated. The saturation power, optical gain and optical phase response of a QD SOA has been analyzed numerically using a rate equation model of quantum dots embedded in a wetting layer. The calculated response is used to model the performance of the logic gates. Impacts of injection current and the input signal power on system quality factor have been studied. For the parameters used in this paper, all-optical logic gates using QD-SOA is capable of operating at speeds of ~ 250Gb/s.

All-optical xor operation has been demonstrated using a semiconductor optical amplifier Mach-Zehnder interferometer (SOA-MZI) and delayed interferometer (DI) at 80 Gb/s. The DI is based on a polarization maintaining loop (PML) mirror. The results show using the PML-DI in series with the SOA-MZI improves the pulse quality of the xor result

Mode locked fiber lasers are important for generation of short pulses for future high speed optical time division multiplexed (OTDM) transmission systems. The design and performance of mode locked fiber lasers are described in this talk.

In this work, an optical short pulse generator is designed consisting of a pulse compressor and cascaded notch filter type repetition rate doublers. The performance characteristics such as pulsewidth and peak power as a function of design parameters are studied. The pulse compressor is optimized based on the simulation results. The 6-ps-wide pulses at 20-GHz repetition rate directly generated from mode-locked fiber laser are compressed to 1.25-ps-wide pulses. Using a set of polarization-maintaining fiber loop mirrors, the repetition rate is quadrupled and stable 1.45-ps-wide pulses at 80 GHz are achieved.

The performance of all-optical XOR gate based on quantum-dot (QD) SOA MZI has been simulated. The saturation power, optical gain and phase response of a QD SOA has been analyzed numerically using a rate equation model of quantum dots embedded in a wetting layer. The calculated response is used to model the XOR performance. For the parameters used here, XOR operation at ~ 250 Gb/s is feasible using QD based Mach-Zehnder interferometers. The speed is limited by the relaxation time from wetting layer to the quantum dots.

In this paper, we demonstrate clock recovery from a patterned 160Gb/s optical-time-division-multiplexed (OTDM) return-to-zero (RZ) data stream. A cascaded LiNbO3 Mach-Zehnder modulator is employed as an efficient optical-electrical mixer. A phase-locked-loop (PLL) is used to lock the cross-correlation component between the optical signal and a local oscillating signal. As a result, clock signal at 10GHz is extracted from the 160Gb/s optical TDM signal. The measured root-mean-square (RMS) timing jitter of the 10GHz; clock signal is similar to 130fs.

A multiwavelength fiber laser source is demonstrated with a semiconductor optical amplifier as the gain medium. A delayed interferometer is incorporated in the ring cavity serving as a comb-like multichannel filter. A stable 75-wavelength simultaneous operation spaced at 40 GHz with the extinction ratio of 40 dB is achieved. By tuning the cavity loss, the center wavelength of the generated lasing waveband could be varied by as much as 20 nm.

All-optical OR operation has been demonstrated using a semiconductor optical amplifier (SOA) and delayed interferometer (DI) at 20 Gb/s and 40 Gb/s. The DI is based on a polarization maintaining loop mirror. Q-factor of the operation is discussed through the numerical simulations. The results show the OR gate operation rate is limited by the carrier lifetime and the input pulse energy.

All optical XOR, AND, and, OR functionality has been demonstrated experimentally using semiconductor optical amplifier (SOA) based devices at 40 Gb/s, 80 Gb/s. The performance of the optical logic operations has been analyzed by solving the rate equation of the SOA numerically. The high-speed operation is limited by the gain and phase recovery times in the SOA. In order to solve these limitations, a differential scheme for XOR operation has been experimentally investigated. This scheme is potentially capable of XOR operation to > 100 Gb/s.

In this work, we demonstrate clock recovery from a patterned 160Gb/s optical-time-division-multiplexed (OTDM) return-to-zero (RZ) data stream. A cascaded LiNbO3 Mach-Zehnder modulator is employed as an efficient optical-electrical mixer. A phase-locked-loop (PLL) is used to lock the cross-correlation component between the optical signal and a local oscillating signal. As a result, clock signal at 10GHz is extracted from the 160Gb/s optical TDM signal. The measured root-mean-square (RMS) timing jitter of the 10GHz clock signal is ~ 130fs.

All-optical XOR functionality has been demonstrated experimentally using an integrated SOA-based Mach-Zehnder interferometer (SOA-MZI) at 20 and 40 Gb/s. The performance of the XOR results has been analyzed by solving the rate equation of the SOA numerically. The high-speed operation is limited by the carrier lifetime in the SOA. In order to solve the limitations imposed by carrier lifetime, a differential scheme for XOR operation has been experimentally investigated. This scheme is potentially capable of XOR operation to >100 Gb/s.

In this paper, we present a scheme for extracting a 10GHz clock from the 80Gb/s optical time division multiplexed (OTDM) return to zero (RZ) data stream. The proposed clock recovery is based on the offset locking technique. By using the input data composed of a repeating "10100000" pattern, residue jitter free operation for clock recovery is demonstrated. The method utilizes a LiNbO3 Mach-Zehnder (MZ) intensity modulator for cross-correlation detections.

In this paper, we demonstrate clock recovery from a patterned optical-time-division-multiplexed (OTDM) return-to-zero (RZ) data stream. A cascaded LiNbO3 Mach-Zehnder modulator is employed as an efficient optical-electrical mixer. A phase-locked-loop (PLL) is used to lock the cross-correlation component between the optical signal and a local oscillating signal. As a result, clock signal at 10GHz is extracted from the 160Gb/s optical TDM signal. The measured root-mean-square (RMS) timing jitter of the 10GHz clock signal is ~130fs.

We demonstrate simultaneous stabilized operation of a mode locked ring fiber laser at two wavelengths. At one of the wavelengths the mode locked operation is at 10 GHz and it is at 40 GHz at the second wavelength. The laser has an intracavity LiNbO3 modulator driven at 10 GHz. The 40 GHz pulses are obtained by rational harmonic mode locking. Pulses with widths in 5 to 8 ps range are obtained.

Gain and output power as a function of pump power has been calculated for short Er/Yb doped single mode fibers for various fiber parameters. The calculation shows (i) small signal gain increases with increasing Er concentration, (ii) long fiber lengths provide both higher gain and higher output, (iii) calculated gain is larger for higher Er emission cross section, and, (iv) > 25 dB gain is feasible for 5 cm long fiber for Er and Yb concentrations of 3 X 10(26) m(-3) and 1.2 X 10(27) m(-3).