Detection of polymorphisms among the parents of the ISF segregating populations IF7310828 x P. ferganensis (F1) and IF7310828 x P72 (BC1) was performed using probes from different genomic and cDNA libraries provided by IRTA, Cabrils (Spain), Clemson University (USA) and ISF itself. Of the 725 probe/restriction enzyme combinations tested, 107 (14.8%) showed polymorphism among the parents and, of these, 73 (68.2%) were segregating in the F1 and 95 (88.8%) in the BC1 populations. Of the 154 probes tested, 58 (37.7%) showed polymorphism with at least one restriction enzyme. Morphological and agronomical traits, such as internode length, glandular foliage, blooming and ripening time, fruit size and flesh adhesion, were recorded in the field on the BC1 segregating population.

The use of DNA-based molecular markers allowed to develop linkage map for identifying and localizing important genes in several plant species. The final purpose of this work is to develop a saturated genetic linkage map in peach in order to identify ergonomically important traits, such as disease resistance and fruit quality, for use in marker assisted selection programs.