Uterine remodeling during pregnancy and repair postpartum are fundamental to the successful propagation of eutherian species. The most drastic remodeling occurs in species with invasively implanting embryos, including humans and mice. During embryo implantation, embryonic trophoblasts breach the epithelium, penetrating into the stroma. Stromal cell decidualization, which is critical for the establishment and maintenance of early pregnancy, occurs throughout the implantation site. Trophoblasts further invade into and remodel uterine spiral arteries, which is necessary for placental formation. The uterus increases in size up to 24-fold, which is largely attributed to myometrial expansion. Uterine changes that occur during pregnancy must then be resolved postpartum. Following parturition, the uterus repairs the remodeled tissue in the process of uterine involution. During involution, the majority of the endometrium is regenerated to replace the tissue that is shed postpartum. The myometrium returns to the pre-gravid state which is thought to occur through apoptosis and autophagy of smooth muscle cells. Although we understand the general process of postpartum uterine involution, the detailed mechanisms, particularly the role of putative stem cells, are poorly understood. This review discusses the evidence for the existence of epithelial, stromal and myometrial stem cells and their role in uterine involution. Gaps in knowledge and areas for future research are also considered. Studies of both postpartum and menstrual uterine repair, which likely involve similar mechanisms, are described under the broad definition of uterine involution. Although the primary focus of this review is human, mouse models are discussed to provide additional information.

The article examines the impact differences between producing concrete and asphalt. Both materials are widely used in the construction industry. Construction activities account for a large portion of greenhouse gases. Therefore, it is important to consider the Life Cycle Analysis (LCA) to reduce environmental impacts. In this study, the material processes were inputted into an LCA program called SimaPro. The database used for the study was Ecoinvent as it is one of the major databases within SimaPro. The materials were compared against impacts per kg of material produced as the functional unit. Each process was created using the materials, energy and transportation required to produce the materials. Waste streams were also included in the process to determine the impacts after the product was done with its useful life. Using the ReCiPe method, an LCA was conducted. Midpoint and endpoint categories were examined for both the productions. The processes had similar results for the human health and ecosystems categories; however asphalt was marginally higher for both. Asphalt had exceeded concrete in the resource impact category by 100 mPt. The results indicate that concrete is the more sustainable building material. Determination of various impacts of the materials is important for material selection.

Local recycled water (LRW) can potentially contribute to resilient and sustainable urban water services critical to liveable cities. Investment in these systems has increased rapidly in Australia in the past 10 years, yet public and private investment in these systems can still be difficult, complex, costly and risky. An in depth case study analysis of Sydney, revealed that while the local policy, institutional and regulatory environment is on the surface conducive to the uptake of local recycled water, actual practice has surprisingly mitigated against further and broader investment in these systems. These instruments are often counteracted by multiple opposing levers that in some instances were developed for entirely different purposes. The generalizable insight is that a systematic, systemic, detailed review of these instruments and levers can reveal unexpected contradictions and provide a strong and defensible base from which to develop strategies to address unintended consequences and remove barriers to future investment.

Small-scale or local recycled water systems are increasingly being installed in urban centers in Australia, and throughout the world. These (often private) systems are in building basements, parks, on industrial sites and within small communities that are already serviced by existing public centralized water and wastewater networks. A consistent and fair assessment of the value of such local recycling systems, particularly in relation to centralized extension, augmentation and replacement, has proved to be problematic. This paper reveals why. It suggests that the traditional characterization of impacts into social, environmental, economic and at times technical groupings misses a key aspect in understanding the relative costs, benefits and risks of these systems: their distribution across the wide range of stakeholder groups. This paper proposes that accounting for the distribution of impacts is critical for assessments that include options of different scales and different levels of responsibility as there is a significant difference in the impact distribution between conventional urban water services and small-scale, local recycled water systems. This will help practitioners better understand the consequences of varying the impact distribution, particularly when moving from substantially public responsibility and ownership of assets to a mix of public and private responsibility and ownership.

A variety of microbially mediated metabolic pathways impact biogeochemical cycling in terrestrial subsurface environments. However, the role that viruses have in influencing microbial mortality and microbial community structure is poorly understood. Here we investigated the production of viruses and change in microbial community structure within shallow alluvial aquifer sediment slurries amended with (13)C-labeled acetate and nitrate. Biostimulation resulted in production of viruses concurrent with acetate oxidation, (13)CO2 production and nitrate reduction. Interestingly, change in viral abundance was positively correlated to acetate consumption (r(2)=0.6252, P<0.05) and (13)CO2 production (r(2)=0.6572, P<0.05); whereas change in cell abundance was not correlated to acetate consumption or (13)CO2 production. Viral-mediated cell lysis has implications for microbial community structure. Betaproteobacteria predominated microbial community composition (62% of paired-end reads) upon inoculation but decreased in relative abundance and was negatively correlated to changes in viral abundance (r(2)=0.5036, P<0.05). As members of the Betaproteobacteria decreased, Gammaproteobacteria, specifically Pseudomonas spp., increased in relative abundance (82% of paired-end reads) and was positively correlated with the change in viral abundance (r(2)=0.5368, P<0.05). A nitrate-reducing bacterium, Pseudomonas sp. strain Alda10, was isolated from these sediments and produced viral-like particles with a filamentous morphology that did not result in cell lysis. Together, these results indicate that viruses are linked to carbon biogeochemistry and community structure in terrestrial subsurface sediments. The subsequent cell lysis has the potential to alter available carbon pools in subsurface environments, additionally controlling microbial community structure from the bottom-up.