Ribosomes isolated from N. gonorrhoeae and N. meningitidis were used as skin test antigens in guinea-pigs which had been previously sensitized with killed Neisseria cells in incomplete Freund adjuvant. Intradermal injection of ribosomes from skin test dose N. gonorrhoeae into the skin of animals sensitized to the homologous organism was characterized by a specificity based upon ribosomal skin test dose 50's (STD50) sensitivity which was at least five-fold greater than that of the animals sensitized to the heterologous organism. The sensitized guinea-pigs had lost their skin-test reactivity within 3 months of the cessation of immunization but regained it immediately on reimmunization. The results of this model system indicate that gonococcal ribosomes may be used as antigens in skin testing or other testing modes in gonorrhoea.

Ribosomal subunits from Mycobacterium smegmatis were analyzed by using sedimentation velocity, sedimentation equilibrium, and acrylamide gel electrophoresis experiments. These s 0.w values for the subunits are 48.7 S and 28.1 S . The molecular weight of the 49 S subunit is about 1.65 × 10 6 , and that of the 28 S subunit is 7.8 × 10 5 . Both subunits contain about 37% protein and 63% ribonucleic acid. A protein-deficient particle having an s 0.w value of 15.7 S contains about 11% protein and 89% ribonucleic acid. Skin tests showed all subunits and proteins to be active as agents in provoking delayed hypersensitivity, but the 16 S protein-deficient particle, as well as the proteins derived from it, was more specific than the subunits themselves.

Ribosomal fractions obtained from Mycobacterium bovis (BCG) and M. smegmatis (strain butyricum ) were studied to determine their antigenicity, their ability to stimulate the production of soluble mediators of delayed hypersensitivity (in vitro correlates) by sensitized peritoneal exudate cells, and the antigenic relations of ribosomal antigens of BCG to BCG protoplasm and H37Rv culture filtrates. The crude ribosomes and the 50-30S ribosomal subunit pool obtained from each of the organisms induced both delayed and immediate hypersensitivity when injected in incomplete Freund adjuvant into rabbits, and skin reactions could be elicited in sensitized rabbits with those antigens. The crude ribosomes and 50-30S ribosomal subunit pool of M. smegmatis stimulated lymphocytes of guinea pigs sensitized with viable organisms to produce macrophage migration inhibition factor. Comparable ribosomal fractions from BCG bacilli caused lymphocytes of guinea pigs sensitized with viable M. bovis (BCG) to produce skin reactive factor. Immunoelectrophoretic studies showed that H37Rv culture filtrate, protoplasm, crude ribosomes, and 50-30S ribosomal subunits of BCG contain multiple precipitinogens and that many of these were shared between the different antigen systems. Comparative electrophoresis revealed that BCG protoplasm and H37Rv culture filtrate shared a major portion of their components with each other and relatively few with ribosomal systems. The ribosomal systems shared the major portion of their components with each other and relatively few with the other antigen systems.

Swiss mice injected subcutaneously with suspensions of spleen cells or an extract of spleens from mice infected with Friend virus develop resistance to subsequent intravenous inoculation of Friend virus. A single injection of either Friend virus or Friend cells induces resistance. Immunized mice display resistance when challenged 6 months after immunization and survive for at least 20 weeks after infection. Neutralization tests indicate that serum, but not lymphoid cells of resistant animals, can neutralize Friend virus. In vitro neutralization tests indicate that residence of virus within the peritoneal cavity of immune mice for 1 h sharply reduces the infective titer of the virus.

Angora, New Zealand, and Dutch-belted rabbits were tested for their susceptibility to graded doses of Herpesvirus hominis type 2 administered by vaginal instillation, intracorneal injection, and scarification of the cornea. Central nervous system involvement and death occurred after infection by the various routes employed. Prior immunization of Dutch-belted and New Zealand rabbits with viable Mycobacterium bovis (BCG) cells injected intravenously provided protection against subsequent infections with type 2 virus.

Journal Article Effect of Normal Serum and Antithymocyte Serum on Friend Disease in Mice Get access C. L. Larson, C. L. Larson Stella Duncan Memorial Institute, University of Montana, Missoula, Montana 59801 Search for other works by this author on: Oxford Academic PubMed Google Scholar R. N. Ushijima, R. N. Ushijima Stella Duncan Memorial Institute, University of Montana, Missoula, Montana 59801 Search for other works by this author on: Oxford Academic PubMed Google Scholar R. E. Baker, R. E. Baker Stella Duncan Memorial Institute, University of Montana, Missoula, Montana 59801 Search for other works by this author on: Oxford Academic PubMed Google Scholar M. B. Baker, M. B. Baker Stella Duncan Memorial Institute, University of Montana, Missoula, Montana 59801 Search for other works by this author on: Oxford Academic PubMed Google Scholar C. A. Gillespie C. A. Gillespie Stella Duncan Memorial Institute, University of Montana, Missoula, Montana 59801 Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 48, Issue 5, May 1972, Pages 1403–1407, https://doi.org/10.1093/jnci/48.5.1403 Published: 01 May 1972 Article history Received: 02 November 1971 Accepted: 19 January 1972 Published: 01 May 1972

Ribosomes and ribosomal subunits of Mycobacterium bovis (strain BCG) and M. smegmatis have been isolated and employed as skin test antigens in guinea pigs sensitized with homologous or heterologous organisms. Ribosomes and ribosomal subunits were found to be potent antigens for skin test purposes, and the 30S subunits were found to be more specific and active than the 50S subunits.

Mice immunized with Mycobacterium bovis (BCG) vaccine (1) develop resistance to infection with Friend's disease virus (FDV). Sjögren and Ankerst (2), and Lemond and Clode (3, 4) immunized mice with BCG during the latent period of adenovirus infection and noted the sparing effect of immunization upon development of tumors. Mathé et al. (5, 6), Amiel (7), and Reif and Kim (8) found that BCG was effective in raising resistance to leukemia L1210 in mice. Schwartz et al. (9) and Zbar et al. (10, 11) studied the relationship of BCG vaccination to the occurrence of sarcomas due to the murine sarcoma virus of Moloney and to tumors in guinea pigs injected with ascites cells obtained from a line of chemically induced tumors. Maté et al. (12, 13) have employed BCG immunotherapy in the treatment of human leukemia and Morton et al. (14, 15) have used this form of therapy to treat malignant melanoma in man. On the basis of the above evidence, immunotherapy was initiated in two groups of mice infected with FDV either 1 or 3 weeks prior to BCG immunization. In the first experiment, 79 mice were injected intravenously (iv) with 0.2 ml of a 10-2.3 dilution of FDV and 79 animals were retained as controls. Three weeks later, 39 of the infected mice and 40 of the normal mice were immunized by iv injection of BCG (4 × 106 vu). At intervals of 3, 4, and 5 weeks after immunization mice from each of the above groups were tested for delayed hypersensitivity by injection of 5.0 μg of PPD into the footpad of the right hind leg. Twenty-four hours later the reactions were recorded, the animals were killed, and the spleen and body weight determined.

Research Articles| July 21 2009 Delayed-Type Skin Reactions in Guinea Pigs Sensitized with Various Acid-Fast Bacilli and Tested with Protoplasms from Homologous and Heterologous Organisms Subject Area: Immunology and Allergy C.L. Larson; C.L. Larson Stella Duncan Memorial Institute, University of Montana, Missoula, Mont. Search for other works by this author on: This Site PubMed Google Scholar R.E. Baker; R.E. Baker Stella Duncan Memorial Institute, University of Montana, Missoula, Mont. Search for other works by this author on: This Site PubMed Google Scholar M.B. Baker M.B. Baker Stella Duncan Memorial Institute, University of Montana, Missoula, Mont. Search for other works by this author on: This Site PubMed Google Scholar International Archives of Allergy and Applied Immunology (1968) 34 (3): 283–292. https://doi.org/10.1159/000230119 Article history Published Online: July 21 2009 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation C.L. Larson, R.E. Baker, M.B. Baker; Delayed-Type Skin Reactions in Guinea Pigs Sensitized with Various Acid-Fast Bacilli and Tested with Protoplasms from Homologous and Heterologous Organisms. International Archives of Allergy and Applied Immunology 1 March 1968; 34 (3): 283–292. https://doi.org/10.1159/000230119 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsInternational Archives of Allergy and Applied Immunology Search Advanced Search Article PDF first page preview Close Modal This content is only available via PDF. 1968Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. You do not currently have access to this content.