Cell cultures of normal adult mouse tissue origin, propagated in vitro for brief periods (34–85 days), exhibited “spontaneous” malignant transformation, as indicated by their ability to produce malignant metastasizing and transplantable tumors following injection into isogeneic and allogeneic normal adult hosts. This malignant transformation is associated with the appearance of a tumor-specific antigen. The demonstration of antigenicity and malignancy of cultured cells depended on the site of implantation in the host. Primary intraperitoneal cell grafts usually resulted in death of the hosts, indicating no effective immune response had occurred. Primary subcutaneous cell grafts produced tumors which frequently regressed and immunized the host against a subsequent intraperitoneal graft. Therefore, malignancy of the cells is not only a property of the cell, but is also a function of cell graft-host interaction. A mechanism by which the site of cell implantation may affect the graft-host interaction is discussed.

Annals of the New York Academy of SciencesVolume 76, Issue 3 p. 413-441 A COMPARATIVE STUDY OF THE USE OF MICROORGANISMS IN THE SCREENING OF POTENTIAL ANTITUMOR AGENTS* G. E. Foley, G. E. Foley Laboratories of Microbiology, The Children's Cancer Research Foundation, and the Department of Pathology, Harvard Medical School, at The Children's Medical Center, Boston, Mass. Member, Subcommittee on Microbiology, Screening Panel, Cancer Chemotherapy National Service Center, National Cancer Institute, Public Health Service, Bethesda, Md.Search for more papers by this authorR. E. McCarthy, R. E. McCarthy Laboratories of Microbiology, The Children's Cancer Research Foundation, and the Department of Pathology, Harvard Medical School, at The Children's Medical Center, Boston, Mass.Search for more papers by this authorV. M. Binns, V. M. Binns Laboratories of Microbiology, The Children's Cancer Research Foundation, and the Department of Pathology, Harvard Medical School, at The Children's Medical Center, Boston, Mass.Search for more papers by this authorE. E. Snell, E. E. Snell Department of Biochemistry, University of California, Berkeley, Calif. Member, Subcommittee on Microbiology, Screening Panel, Cancer Chemotherapy National Service Center, National Cancer Institute, Public Health Service, Bethesda, Md.Search for more papers by this authorB. M. Guirard, B. M. Guirard Department of Biochemistry, University of California, Berkeley, Calif.Search for more papers by this authorG. W. Kidder, G. W. Kidder Biological Laboratory, Amherst College, Amherst, Mass. Member, Subcommittee on Microbiology, Screening Panel, Cancer Chemotherapy National Service Center, National Cancer Institute, Public Health Service, Bethesda, Md.Search for more papers by this authorV. C. Dewey, V. C. Dewey Biological Laboratory, Amherst College, Amherst, Mass.Search for more papers by this authorP. S. Thayer, P. S. Thayer Biological Laboratory, Arthur D. Little, Inc., Cambridge, Mass.Search for more papers by this author G. E. Foley, G. E. Foley Laboratories of Microbiology, The Children's Cancer Research Foundation, and the Department of Pathology, Harvard Medical School, at The Children's Medical Center, Boston, Mass. Member, Subcommittee on Microbiology, Screening Panel, Cancer Chemotherapy National Service Center, National Cancer Institute, Public Health Service, Bethesda, Md.Search for more papers by this authorR. E. McCarthy, R. E. McCarthy Laboratories of Microbiology, The Children's Cancer Research Foundation, and the Department of Pathology, Harvard Medical School, at The Children's Medical Center, Boston, Mass.Search for more papers by this authorV. M. Binns, V. M. Binns Laboratories of Microbiology, The Children's Cancer Research Foundation, and the Department of Pathology, Harvard Medical School, at The Children's Medical Center, Boston, Mass.Search for more papers by this authorE. E. Snell, E. E. Snell Department of Biochemistry, University of California, Berkeley, Calif. Member, Subcommittee on Microbiology, Screening Panel, Cancer Chemotherapy National Service Center, National Cancer Institute, Public Health Service, Bethesda, Md.Search for more papers by this authorB. M. Guirard, B. M. Guirard Department of Biochemistry, University of California, Berkeley, Calif.Search for more papers by this authorG. W. Kidder, G. W. Kidder Biological Laboratory, Amherst College, Amherst, Mass. Member, Subcommittee on Microbiology, Screening Panel, Cancer Chemotherapy National Service Center, National Cancer Institute, Public Health Service, Bethesda, Md.Search for more papers by this authorV. C. Dewey, V. C. Dewey Biological Laboratory, Amherst College, Amherst, Mass.Search for more papers by this authorP. S. Thayer, P. S. Thayer Biological Laboratory, Arthur D. Little, Inc., Cambridge, Mass.Search for more papers by this author First published: December 1958 https://doi.org/10.1111/j.1749-6632.1958.tb54862.xCitations: 54 † The work reported in this paper was supported in part by Research Grant CY-333S and by Contract SA-43-ph-10S3 between The Children's Cancer Research Foundation, Boston, Mass., and the National Cancer Institute, Public Health Service, Bethesda, Md.; by Contract SA-43-ph-1504 between the Regents of the University of California, Berkeley, Calif., and the National Cancer Institute; by Contract SA-43-ph-1042 between the Board of Trustees of Amherst College, Amherst, Mass., and the National Cancer Institute; and by Contract SA-43-ph-1087 between Arthur D. Little, Inc., Cambridge, Mass., and the National Cancer Institute. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Reference 1 Investigation of Diverse Systems for Cancer Chemotherapy Screening . 1955 . A. Gellhorn & E. Hirschberg , Eds . Cancer Research. Suppl. No. 3 : 1 – 112 . 2 Foley , G. E. . 1956 . Current Research in Cancer Chemotherapy. 6 : 3 – 22 . 3 Kidder , G. W. , V. C. Dewey , R. E. Parks , Jr. & G. L. Woodside . 1949 . Science. 109 : 511 – 514 . 4 Eagle , H. & G. E. Foley . 1958 . Cancer Research . In press . 5 Felton , E. A. & C. F. Niven , Jr. . 1953 . J. 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Summary report on Contract SA-43-ph-1053. Cancer Chemotherapy Natl . Service Center. Public Health Service . Bethesda , Md . 28 Snell , E. E. & B. M. Guirard . 1957 . Report on the screening of 200 compounds for inhibitory activity against microorganisms . Summary report on Contract SA-43-ph-1504. Cancer Chemotherapy Natl. Service Center. Public Health Service. Bethesda, Md . 29 Kidder , G. W. & V. C. Dewey . 1957 . Summary of ciliate screening . Summary report on Contract SA-43-ph-1042. Cancer Chemotherapy Natl . Service Center. Public Health Service . Bethesda , Md . 30 Thayer , P. S. . 1957 . Inhibition of the growth of Neurospora by selected chemical compounds . Summary report on Contract SA-43-ph-1087. Cancer Chemotherapy Natl . Service Center. Public Health Service . Bethesda , Md . 31 Hutchings , B. L. , J. H. Mowat , J. J. Oleson , E. L. R. Stokstad , J. H. Boothe , C.W. Waller , R. B. Angier , J. Semb & Y. Subbarow . 1947 . J. Biol. Chem. 170 : 323 – 328 . 32 Survey by Staff of Cancer Chemotherapy National Service Center (Public Health Service) . 1958 . I. Compilation of anti-tumor data from the literature. II. Results of antitumor screening tests under the auspices of the Center . (Compare with appendix to this monograph.) . 33 Foley , G. E. , H. Eagle , E. E. Snell , G. W. Kidder & P. S. THAYER . 1958 . Ann. N. Y. Acad. Sci. 76 ( 3 ): 952 – 960 . 34 Skipper , H. E. . 1953 . Cancer Research. 13 : 545 – 551 . Citing Literature Volume76, Issue3Screening Procedures for Experimental Cancer ChemotherapyDecember 1958Pages 413-441 ReferencesRelatedInformation