Pine tree substrate (PTS), for container plant production, is a relatively new alternative to the commonly used pine bark and peat substrates. Fertility management requires knowledge of nitrogen transformations in this new substrate. The objective of this study was to document the occurrence of nitrification in PTS and to determine if nitrification and density of nitrifying microorganisms are affected by substrate storage time and lime and peat amendments. Pine tree substrate was manufactured by hammermilling chips of ≈15-year-old loblolly pine trees ( Pinus taeda L.) through two screen sizes, 4.76 mm (PTS) and 15.9 mm amended with peat (3PTS:1 peat, v:v, PTSP). Pine tree substrate and PTSP were amended with lime at five rates and a peat–perlite mix (4 peat:1 perlite, v:v, PL) served as a control treatment for a total of 11 treatments. Substrates were prepared, placed in plastic storage bags, and stored on shelves in an open shed in Blacksburg, VA. Subsamples were taken at 1, 42, 84, 168, 270, and 365 days after storage. At each subsampling day, each substrate was placed into 12 1-L containers. Six of the 12 were left fallow and six were planted with 14-day-old marigold ( Tagetes erecta L. ‘Inca Gold’) seedlings; all containers were placed on a greenhouse bench. Substrates were also collected for most probable number (MPN) assays for nitrifying microorganism quantification. Substrate solution pH, electrical conductivity (EC), ammonium-N (NH 4 -N), and nitrate-N (NO 3 -N) were measured on fallow treatments. Marigold substrate solution pH, EC, NH 4 -N, and NO 3 -N were measured after 3 weeks of marigold growth. Nitrate-N was detected in fallow containers at low concentrations (0.4 to 5.4 mg·L −1 ) in PTS in all limed treatments at all subsampling days, but in the non-limed treatment, only at Days 270 and 365. Nitrate-N was detected in the fallow containers at low concentrations (0.7 to 13.7 mg·L −1 ) in PTSP in the 4- and 6-kg·m −3 lime rates at all subsampling days. Nitrite-oxidizing microorganisms were present in PTS at all subsampling days with the highest numbers measured at Day 1. Ammonium-to-nitrate ratios for the marigold substrate solution extracts for both PTS and PTSP decreased as pH increased. This study shows that nitrifying microorganisms are present and nitrification occurs in PTS and PTSP and is positively correlated to substrate pH.

Pine tree substrate (PTS) is a relatively new alternative to the commonly used pine bark and peat-based substrates for container crop production. Physical and chemical properties of freshly manufactured PTS have been studied; however, this new substrate will sometimes be manufactured and stored for later use by growers. The objective of this research was to determine how chemical and physical properties of PTS were affected by storage duration with or without amendments of limestone or peatmoss. We also studied how the growth of marigold was influenced by PTS storage time and by lime and peat amendments. Substrate properties studied were pH, cation exchange capacity (CEC), electrical conductivity (EC), carbon-to-nitrogen ratio (C:N), bulk density (BD), and particle size distribution. Pine tree substrate was manufactured by hammermilling chips of ≈15-year-old loblolly pine trees ( Pinus taeda L.) through two screen sizes, 4.76 mm (PTS) and 15.9 mm [amended with peat (PTSP)]. Pine tree substrate and PTSP were amended with lime at five rates and a peat–perlite mix (PL) served as a control treatment. Substrates were prepared, placed in plastic storage bags, and stored on shelves in an open shed in Blacksburg, VA. Substrates were subsampled at 1, 42, 84, 168, 270, and 365 days after storage. At each subsampling day, twelve 1-L containers were filled with a subsample of each treatment. Six of the 12 were left fallow and six were planted with 14-day-old marigold ( Tagetes erecta L. ‘Inca Gold’) seedlings. Substrate was also collected for analysis of CEC, C:N, BD, and particle size distribution. The pH of non-limed PTS decreased during storage, and at least 1 kg·m −3 lime was needed to maintain PTS pH 5.4 or greater over the 365-day storage period (Day 1 pH = 5.8) and 2 to 4 kg·m −3 was needed to maintain PTSP pH 5.4 or greater for 365 days (Day 1 pH = 5.2). EC measurements were highest at Day 1 (1.02 to 1.21 dS·m −1 ) in all treatments and decreased by Day 42. Cation exchange capacity decreased over time in non-limed PTS and PTSP. Carbon-to-nitrogen ratio and BD remained the same over time for all treatments. There were minor changes in particle size distribution for limed PTS. Marigold growth in all limed PTS and PTSP treatments was equal to or greater than in PL, except at Day 1; the lower growth in PTS and PTSP at Day 1 compared with PL suggests that freshly manufactured PTS may contain a phytotoxic substance that was not present in PTS by Day 42. Pine tree substrate and PTSP are relatively stable when stored as described previously, except for a pH decrease that can be prevented with additions of lime before storage.

The use of freshly harvested and processed pine trees as a container substrate for greenhouse and nursery crop production is a relatively new concept, and fundamental knowledge of the construction of a pine tree substrate (PTS) for optimal physical properties is insufficient. Therefore, this research was conducted to determine the influence of mixing PTSs produced with different wood particle sizes and adding other amendments to PTS on substrate physical properties and plant growth compared with traditional substrates. Coarse pine wood chips produced from 15-year-old loblolly pine trees ( Pinus taeda L.) were ground in a hammermill fitted with either a 4.76-mm screen or with no screen (PTS-NS) allowing a fine and a coarse particle PTS to be produced. Increasing proportions of the finer (4.76-mm) PTS to the coarser PTS (PTS-NS) resulted in increased container capacity (CC) and shoot growth of ‘Inca Gold’ marigold ( Tagetes erecta L.). In another study, PTSs were manufactured in a hammermill fitted with different screen sizes: 4.76, 6.35, 9.54, or 15.8 mm as well as PTS-NS. After being hammermilled, each of the five PTSs was then amended (by mixing) with 10% sand (PTS-S), 25% peatmoss (PTS-PM), or left unamended. Pine tree substrates were also produced by adding 25% aged pine bark (PB) to pine wood chips before being ground in a hammermill with each of the five screen sizes mentioned (PTS-HPB). These five substrates were used unamended as well as amended with 10% sand after grinding (PTS-HPBS). Control treatments included peat-lite (PL) and 100% aged PB for a total of 27 substrates evaluated in this study. Container capacity and marigold growth increased as screen size decreased and with the additions of peatmoss (PTS-PM) or hammering with PB (PTS-HPB) to PTS. Container capacity for all substrates amended with peatmoss or PB was within the recommended range of 45% to 65% for container substrates, but only with the more finely ground PTS-4.76-mm resulted in marigold growth comparable to PL and PB. However, when the PTS-NS was amended by mixing in 25% peat or hammering with 25% PB, growth of marigold was equal to plants grown in PL or PB. In a third study, hammering PTS-NS with 25% PB followed by the addition of 10% sand increased dry weight of both azalea ( Rhododendron × hybrida ‘Girard Pleasant White’) and spirea ( Spiraea nipponica Maxim. ‘Snowmound’) resulting in growth equal to plants grown in 100% PB. This work shows that amending coarsely ground PTS with finer particle PTS or with other materials (peatmoss, aged PB, or sand) can result in a substrate with comparable physical properties such as CC and plant growth compared with 100% PL or PB.

The objective of this study was to evaluate the landscape performance of annual bedding plants grown in a ground pine tree substrate (PTS) produced from loblolly pine trees ( Pinus taeda ) or in ground pine bark (PB) when transplanted into the landscape and grown at three different fertilizer rates. Begonia ( Begonia × semperflorens-cultorum ) ‘Cocktail Vodka’, coleus ( Solenostemen scutellarioides ) ‘Kingswood Torch’, impatiens ( Impatiens walleriana ) ‘Dazzler White’, marigold ( Tagetes erecta ) ‘Bonanza Yellow’, petunia ( Petunia × hybrid ) ‘Wave Purple’, salvia ( Salvia splendens ) ‘Red Hot Sally’, and vinca ( Catharanthus roseus ) ‘Cooler Pink’ were evaluated in 2005, and begonia ‘Cocktail Whiskey’, marigold ‘Inca Gold’, salvia ‘Red Hot Sally’, and vinca ‘Cooler Pink’ were evaluated in 2006 and 2007. Landscape fertilizer rates were 1 lb/1000 ft 2 nitrogen (N) in 2005 and 0, 1, and 2 lb/1000 ft 2 N in 2006 and 2007. Visual observations throughout each year indicated that all species, whether grown in PTS or PB, had comparable foliage quality in the landscape trial beds during the growing period. With few exceptions, dry weight and plant size for all species increased with increasing fertilizer additions, regardless of the substrate in which the plants were grown. For the unfertilized treatment, when comparing plant dry weight between PB and PTS for each species and for each year (eight comparisons), PTS-grown plant dry weight was less than PB-grown plants in three out of the eight comparisons. However, there were fewer differences in plant dry weight between PTS- and PB-grown plants when fertilizer was applied (PTS-grown plants were smaller than PB-grown plants in only 2 of the 16 comparisons: four species, two fertilizer rates, and 2 years), indicating that N immobilization may be somewhat of an issue, but not to the extent expected. Therefore, the utilization of PTS as a substrate for the production of landscape annuals may be acceptable in the context of landscape performance.

The objective of this study was to evaluate a pine tree substrate (PTS) for decomposition, changes in physical and chemical properties, and substrate carbon dioxide (CO 2 ) efflux (microbial activity) during a long-term production cycle under outdoor nursery conditions. Substrates used in this study were PTS constructed using a 4.76-mm hammer mill screen and aged pine bark (PB). Plastic nursery containers were filled with each substrate and amended with either 4.2 or 8.4 kg·m −3 Osmocote Plus fertilizer and planted with Cotoneaster horizontalis or left fallow. Substrate solution chemical properties and nutrient concentrations were determined each month during the summers of 2006 and 2007 in addition to measuring substrate CO 2 efflux (μmol CO 2 /m −2 ·s −1 ) as an assessment of microbial activity. Substrate breakdown (decomposition) was determined with particle size analysis and physical property determination on substrates at the conclusion of the study (70 weeks). Substrate solution pH was higher in PTS than in PB at both fertilizer rates in 2006, but pH levels decreased over time and were lower in PTS at both fertilizer rates in 2007. Substrate solution electrical conductivity levels, nitrate, phosphorus, and potassium concentrations were all generally higher in PB than in PTS at both fertilizer rates through both years. Pine tree substrate decomposition was higher when plants were present in the containers [evident by an increase in fine substrate particles (less than 0.5 mm) after 70 weeks], but breakdown was equal at both fertilizer rates. Shrinkage of PTS in the presence of plants was equal to the shrinkage observed in PB with plants, but shrinkage was higher in fallow PTS containers than PTS with plants. Substrate air apace (AS) was highest in PTS and container capacity (CC) was equal in PB and PTS at potting. Substrate AS decreased and CC increased in both substrates after 70 weeks but remained in acceptable ranges for container substrates. Substrate CO 2 efflux rates were higher in PTS compared with PB at both fertilizer rates indicating higher microbial activity, thereby increasing the potential for nutrient immobilization and substrate breakdown. This work provides evidence that PTS decomposition is unaffected by fertilizer rate and that substrate shrinkage in containers with plants is similar to PB after two growing seasons (70 weeks), which addresses two major concerns about the use and performance of PTS for long-term nursery crop production. This work also shows that the higher microbial activity in PTS increases the potential of microbial nutrient immobilization, which is likely the reason for the lower substrate nutrient levels reported for PTS compared with PB over 70 weeks.

The objective of this work was to determine and compare the changes in physical properties and substrate shrinkage of a pine tree substrate (PTS) to a traditional peat substrate in fallow containers over time under fertigated greenhouse conditions. Pine tree substrates were produced from loblolly pine trees (Pinus taeda L.) that were chipped, and hammer milled with different screen sizes. Substrates used in this study included peat-lite (PL), PTS produced with a 2.38-mm (3/32-inch) screen (PTS1), and a PTS produced with a 4.76-mm (3/16-inch) screen (PTS2). Containers were filled with the individual substrates, fertilized weekly with 300 ppm N, and maintained under greenhouse conditions for 14 weeks. Initial and final substrate physical properties and substrate shrinkage were determined to evaluate and compare changes in the substrates that occur over time. Initial and final air space (AS) was higher in both PTSs compared to PL and container capacity (CC) of PTS1 was equal to PL initially and at the end of the experiment. The initial and final CC of PTS2 was lower than PL. Substrate shrinkage was not different between PL and PTS1, but greater than shrinkage with the coarser PTS2.

Bioassays with tomato and lettuce seeds and three plant experiments with marigold (Tagetes erecta L.) 'Inca Gold' were conducted in the laboratory and greenhouse, respectively, in order to investigate possibilities of determining and mitigating the effects of phytotoxins in pine tree substrate (PTS). PTS is produced by grinding loblolly pine tree (Pinus taeda L.) logs, and using as horticultural growing substrate. PTS extracts obtained with cold and hot water reduced the germination rate and radicle growth of tomato and lettuce. After the washing, an improvement was monitored for radicle length of both species. The germination index supported the data of radicle length showing a better performance after the substrate washing. Radicle growth of tomato in extracts from PTS used in a plant growth experiment was improved after leaching PTS six times.The performance of marigold plants was similar to bioassays with tomatoes and lettuce. Marigold growth was improved by pretreatments of PTS. Some of these pretreatments could be recommended for use in the manufacturing process for PTS or from growers before planting. However, the best results were shown in a commercial peat-lite substrate.

This work was conducted to evaluate the effect of limestone additions to pine tree substrate (PTS) and PTS amended with peatmoss on pH and plant growth. ‘Inca Gold’ marigold ( Tagetes erecta L.) and ‘Rocky Mountain White’ geranium ( Pelargonium × hortorum L.H. Bailey) were grown in three PTSs—100% PTS, PTS plus 25% peatmoss (v/v), and PTS plus 50% peatmoss (v/v)—made from freshly harvested loblolly pine trees ( Pinus taeda L.) chipped and hammermilled through a 4.76-mm screen and a peatmoss/perlite (4:1 v/v; PL) control. Each substrate was amended with various rates of dolomitic limestone and used to grow marigolds in 10-cm square (l-L) plastic containers and geraniums in round 15-cm (1.25-L) plastic containers in a glasshouse. Regardless of limestone rate, pH was highest in 100% PTS and decreased with peat additions with PL having the lowest pH. As percent peat increased from 25% to 50%, more limestone was required to adjust pH to a particular level showing that PTS is more weakly buffered against pH change than peatmoss. Adding limestone did not increase the growth of marigold in 100% PTS, but additions of limestone did increase growth of marigold when grown in PTS containing peatmoss or in PL. Geranium growth was higher in PTS containing peatmoss (25% or 50%) and PL than in 100% PTS at all limestone rates. This research demonstrates that PTS produced from freshly harvested pine trees has an inherently higher pH than PL, and the additions of peatmoss to PTS require pH adjustment of the substrate for optimal plant growth.

This Journal of Environmental Horticulture article is reproduced with the consent of the Horticultural Research Institute (HRI – www.hriresearch.org), which was established in 1962 as the research and development affiliate of the American Nursery & Landscape Association (ANLA – http://www.anla.org). HRI’s Mission: To direct, fund, promote and communicate horticultural research, which increases the quality and value of ornamental plants, improves the productivity and profitability of the nursery and landscape industry, and protects and enhances the environment.

The objective of this study was to compare substrate solution nitrogen (N) availability, N immobilization, and nutrient leaching in a pine tree substrate (PTS), peat-lite (PL), and aged pine bark (PB) over time under greenhouse conditions. Pine tree substrate was produced from loblolly pine logs ( Pinus taeda L.) that were chipped and hammer-milled to a desired particle size. Substrates used in this study were PTS ground through a 2.38-mm hammer mill screen, PL, and aged PB. A short-term (28-d) N immobilization study was conducted on substrates fertilized with 150 or 300 mg·L −1 NO 3 -N. Substrates were incubated for 4 days after fertilizing and NO 3 -N levels were determined initially and at the end of the incubation. A second medium-term study (10-week) was also conducted to evaluate the amount of N immobilized in each substrate when fertilized with 100, 200, 300, or 400 mg·L −1 N. In addition to determining the amounts of N immobilized, substrate carbon dioxide (CO 2 ) efflux (μmol CO 2 /m −2 ·s −1 ) was also measured as an assessment of microbial activity, which can be an indication of N immobilization. A leaching study on all three substrates was also conducted to determine the amount of nitrate nitrogen (NO 3 -N), phosphorus, and potassium leached over 14 weeks under greenhouse conditions. Nitrogen immobilization was highest in PTS followed by PB and PL in both the short- and medium-term studies. Nitrogen immobilization increased as fertilizer rate increased from 100 mg·L −1 N to 200 mg·L −1 N in PL and from 100 mg·L −1 N to 300 mg·L −1 N for PB and PTS followed by a reduction or no further increase in immobilization when fertilizer rates increased beyond these levels. Nitrogen immobilization was generally highest in all substrates 2 weeks after potting, after which immobilization tended to decrease over the course of several weeks with less of a decrease for PTS compared with PL and PB. Substrate CO 2 efflux levels were highest in PTS followed by PB and PL at each measurement in both the short- and medium-term studies. Patterns of substrate CO 2 efflux levels (estimate of microbial populations/activity) at both fertilizer rates and over time were positively correlated to N immobilization occurrence during the studies. Nitrate leaching over 14 weeks was lower in PTS than in PB or PL through 14 weeks. This work provides evidence of increased microbial activity and N immobilization in PTS compared with PB and PL. Increased N immobilization in PTS explains the lower nutrient (primarily N) levels observed in PTS during crop production and justifies the additional fertilizer required for comparable plant growth to PL and PB. This work also provides evidence of less NO 3 -N leaching in PTS compared with PL or PB during greenhouse crop production despite the higher fertilizer rates required for optimal plant growth in PTS.

‘Prestige’ poinsettias ( Euphorbia pulcherrima Willd. Ex Klotzsch) were grown at different fertilizer rates in three pine tree substrates (PTS) made from loblolly pine trees ( Pinus taeda L.) and a peat-based control. Pine tree substrates were produced from pine trees that were chipped and hammer-milled to a desired particle size. Substrates used in this study included peat-lite (PL), PTS produced with a 2.38-mm screen (PTS1), PTS produced with a 4.76-mm screen (PTS2), and PTS produced with a 4.76-mm screen and amended with 25% peatmoss (v/v) (PTS3). Initial and final substrate physical properties and substrate shrinkage were determined to evaluate changes over the production period. Poinsettias were grown in 1.7-L containers in the fall of 2007 and fertilized at each irrigation with 100, 200, 300, or 400 mg·L −1 nitrogen (N). Shoot dry weight and growth index were higher in PL at 100 mg·L −1 N but similar for all substrates at 300 mg·L −1 N. Bract length was generally the same or longer in all PTS-grown plants compared with plants grown in PL at each fertilizer rate. Postproduction time to wilting was the same for poinsettias grown in PL, PTS1, and PTS3. Initial and final air space was higher in all PTSs compared with PL and container capacity (CC) of PTS1 was equal to PL initially and at the end of the experiment. The initial and final CC of PTS2 was lower than PL. The incorporation of 25% peat (PTS3) increased shoot dry weight and bract length at lower fertilizer rates compared with 4.76 mm PTS alone (PTS2). Substrate shrinkage was not different between PL and PTS1 but greater than shrinkage with the coarser PTS2. This study demonstrates that poinsettia can be successfully grown in a PTS with small particles (2.38-mm screen) or a PTS with large particles (4.76-mm screen) when amended with 25% peatmoss, which results in physical properties (CC and air space) similar to those of PL.

A pine tree substrate (PTS), produced by grinding loblolly pine trees ( Pinus taeda ), offers potential as a viable container substrate for greenhouse crops, but a better understanding of the fertilizer requirements for plant growth in PTS is needed. The purpose of this research was to determine the comparative fertilizer requirements for chrysanthemum ( Chrysanthemum × grandiflora ‘Baton Rouge’) grown in PTS or a commercial peat-lite (PL) substrate. The PTS was prepared by grinding coarse (1-inch × 1-inch × 0.5-inch) pine chips from debarked loblolly pine logs in a hammer mill fitted with 3/16-inch screen. The PL substrate composed of 45% peat, 15% perlite, 15% vermiculite, and 25% bark was used for comparative purposes. Rooted chrysanthemum cuttings were potted in each of the substrates on 15 Oct. 2005 and 12 Apr. 2006 and were glasshouse grown. Plants were fertilized with varying rates of a 20N–4.4P–16.6K-soluble fertilizer ranging from 50 to 400 mg·L −1 nitrogen (N) with each irrigation. Plant dry weights and extractable substrate nutrient levels were determined. In 2005 and 2006, it required about 100 mg·L −1 N more fertilizer for PTS compared to PL to obtain comparable growth. At any particular fertilizer level, substrate electrical conductivity and nutrient levels were higher for PL compared to PTS accounting for the higher fertilizer requirements for PTS. Possible reasons for the lower substrate nutrients levels with PTS are increased nutrient leaching in PTS due to PTS being more porous and having a lower cation exchange capacity than PL, and increased microbial immobilization of N in PTS compared to PL. This research demonstrates that PTS can be used to grow a traditional greenhouse crop if attention is given to fertilizer requirements.