Eph receptor tyrosine kinases are largely known for their involvement in brain development but, as some of these receptor tyrosine kinases are also expressed in adults, their possible role in the mature nervous system has begun to be explored. Evidence for the involvement of Eph receptors in synaptic plasticity, learning and memory is only emerging and needs corroboration. However, it is likely that the actions of Eph kinases in the adult brain will attract significant attention and become a fertile research area, as occurred in the case of the neurotrophins.

Publisher Summary This chapter discusses the application of real-time RT-PCR for quantification of gene expression. The use of real-time RT-PCR methodology permits rapid assay development from validation of RNA sample preparation methods, to validation of the invariance of housekeeping genes. Real-time PCR also provides a quantitative method for moderate to high throughput analysis of gene expression. The ABI instrument permits the analysis of 96 tubes simultaneously, allowing for multiple samples and genes to study in single PCR experiment. The ABI instrument permits the analysis of 96 tubes simultaneously, allowing for multiple samples and genes to be studied in single PCR experiment. This system can produce precise and reliable data which correlates with traditional methods. In addition, the chapter illustrates that real-time RT-PCR technology plays a major role in the future of quantitative gene expression analysis.