Site fidelity is the tendency for animals to repeatedly return to the same locations, either within or between years. Site fidelity enables animals to utilize knowledge of previously visited locations, including assessments of seasonal variations in health and mortality risks (e.g., predation), resource availability, and social benefits such as pairing with previous mates. However, rigidly enacted site fidelity may come with fitness costs in times of rapid habitat change. One group believed to exhibit site fidelity is migratory shorebirds (families Charadriidae and Scolopacidae). Shorebirds that migrate annually via the East Asian-Australasian Flyway have experienced rapid population declines due to habitat loss in Asia. Although high site fidelity may influence these declines under a global change scenario, only limited, species-specific analyses of site fidelity have been previously conducted. Our study used an extensive dataset of 636,167 records of over 84,000 banded individual shorebirds from 1976 to 2025 to present an overview of site fidelity for 12 migratory shorebird species during their nonbreeding season in Australia. We found overall site fidelity of >95% across 10 out of 12 species, with the only exceptions being red knot (Calidris canutus) and sanderling (Calidris alba), with movements often limited to sections of coastline less than 20 km long. Juvenile and immature birds were usually less site faithful than adults. The high site fidelities of most species suggest that local site knowledge is of considerable importance and that individuals forced to relocate to alternative sites will likely incur a cost. Our findings also imply that habitat loss may have immediate population-level consequences through reduced fitness of displaced birds.

Morphological changes concurrent with climate change are increasingly identified in birds, often through decreasing body size and increasing appendage size. Such changes could have thermoregulatory implications, through the improved surface area to body ratio they provide. Due to the role of bird wings in thermoregulation, wing length relative to body mass may be changing as another form of shape-shifting, where increased relative wing length may facilitate increased heat loss as climates warm. We investigated changes in relative wing length on a dataset of nearly 20 000 juvenile shorebirds from 11 species over the past four decades, to determine changes in morphology and whether these are linked to developmental temperatures. Overall, across species, we found that relative wing length increased across the 43-year study period in populations migrating to tropical northern Australia but not in those migrating to temperate southern Australia. Furthermore, we found that changes in relative wing length were not driven by immediate responses to high temperature at the breeding ground during juvenile growth. These results may suggest that relative wing length increases occur in shorebirds occupying already warm climates, where they might potentially be more thermally challenged under further warming, but that such changes are not occurring through plastic mechanisms during development.

Per- and poly-fluoroalkyl substances (PFAS) may threaten wildlife due to their high environmental persistence, toxicity potential and potential to bioaccumulate. Bioaccumulation may be particularly profound in long-lived animals inhabiting higher trophic niches. To date, there is a paucity of data on PFAS bioaccumulation potential in individual wild birds over their lifetime. In this study, we analysed within-individual PFAS contamination in a declining long-distance migratory shorebird, the ruddy turnstone (Arenaria interpres), and the variation in PFAS contamination with age by repeatedly sampling 19 individuals throughout their lives between 2007 and 2022. We found blood-sampled turnstones on their non-breeding grounds in King Island, Tasmania, exhibited no variation of PFAS contamination with age, with low overall circulating PFAS concentrations (<0.015-25 ng/g, median: 0.78 ng/g). Moreover, irrespective of the increased PFAS usage along the East Asian Australasian Flyway over the past two decades, ruddy turnstone survival remained consistent throughout the 15-year sampling period, with no temporal trend in percentage of juveniles in the population. From a conservation perspective, low concentrations of PFAS found in this study are good news as they suggest PFAS alone do not seem to threaten turnstone survival. However, the unknown effects of exposure to mixtures of pollutants may yet threaten turnstones.

There is an ongoing and profound burden of lineage 2.3.4.4b high pathogenicity avian influenza (HPAI) H5N1 on wildlife and poultry, globally. Herein we report the continued absence of HPAI and antibodies against lineage 2.3.4.4b HPAI from October – December 2023, in migratory birds shortly after their arrival in Australia. Given the ever-changing phenotype of this virus, worldwide studies on the occurrence, or here absence of the virus, are of critical importance to understand the virus’ dispersal and incursion risk and development of response strategies. ### Competing Interest Statement The authors have declared no competing interest.

Exposure to pollutants is a potentially crucial but overlooked driver of population declines in shorebirds along the East Asian-Australasian Flyway. We combined knowledge of moult strategy and life history with a standardised sampling protocol to assess mercury (Hg) contamination in 984 individuals across 33 migratory shorebird species on an intercontinental scale. Over one-third of the samples exceeded toxicity benchmarks. Feather Hg was best explained by moulting region, while habitat preference (coastal obligate vs. non-coastal obligate), the proportion of invertebrates in the diet and foraging stratum (foraging mostly on the surface vs. at depth) also contributed, but were less pronounced. Feather Hg was substantially higher in South China (Mai Po and Leizhou), Australia and the Yellow Sea than in temperate and Arctic breeding ranges. Non-coastal obligate species (Tringa genus) frequently encountered in freshwater habitats were at the highest risk. It is important to continue and expand biomonitoring research to assess how other pollutants might impact shorebirds. Over one-third of the sampled shorebirds along the East Asian-Australasian Flyway are facing Hg risk. Tringa genus in South China was at the highest risk. Feather Hg was best explained by feathers' moulting region, while habitat preference, diet, and foraging stratum were less important.

AbstractMuch of the annual cycle of threatened migratory shorebirds is spent in non‐breeding areas, but there have been few studies on how that habitat is used at fine‐scale. Tracking data from 13 endangered far eastern curlews at three Australian non‐breeding locations revealed that home range size and maximum daily displacement varied substantially among study areas and between years. Home range overlap also varied with tidal cycle, generally being lower at low than high tide, though there was considerable variability across time and between sites. Variation in site fidelity may indicate behavioral flexibility but may also suggest that resources critical to survival vary between years. If the latter is the case, protection of what appears to be critical habitat in 1 year may not be adequate in subsequent years, necessitating a precautionary approach to the conservation of habitat extent over the long term. For species like the far eastern curlew, which are exceptionally hard to capture and highly threatened, tracking data should be analyzed from as many perspectives as possible to justify the cost to both researchers and birds. In this study, use of tracking data previously analyzed to reveal migration routes has yielded important insights into their ecology and optimal management.

Animals are predicted to shrink and shape-shift as the climate warms; declining in size, while their appendages lengthen. Determining which types of species are undergoing these morphological changes, and why, is critical to understanding species responses to global change, including potential adaptation to climate warming. We examine body size and bill length changes in 25 shorebird species using extensive field data (>200,000 observations) collected over 46 years (1975-2021) by community scientists. We show widespread body size declines over time, and after short-term exposure to warmer summers. Meanwhile, shorebird bills are lengthening over time but shorten after hot summers. Shrinking and shape-shifting patterns are consistent across ecologically diverse shorebirds from tropical and temperate Australia, are more pronounced in smaller species, and vary according to migration behaviour. These widespread morphological changes could be explained by multiple drivers, including adaptive and maladaptive responses to nutritional stress, or by thermal adaptation to climate warming.

The rapid destruction of natural wetland habitats over past decades has been partially offset by an increase in artificial wetlands. However, these also include wastewater treatment plants, which may pose a pollution risk to the wildlife using them. We studied two long-distance Arctic-breeding migratory shorebird species, curlew sandpiper (Calidris ferruginea, n = 69) and red-necked stint (Calidris ruficollis, n = 103), while on their Australian non-breeding grounds using an artificial wetland at a wastewater treatment plant (WTP) and a natural coastal wetland. We compared pollutant exposure (elements and per- and poly-fluoroalkyl substances/PFASs), disease (avian influenza), physiological status (oxidative stress) of the birds at the two locations from 2011 to 2020, and population survival from 1978 to 2019. Our results indicated no significant differences in blood pellet pollutant concentrations between the habitats except mercury (WTP median: 224 ng/g, range: 19-873 ng/g; natural wetland: 160 ng/g, 22-998 ng/g) and PFASs (total PFASs WTP median: 85.1 ng/g, range: <0.01-836 ng/g; natural wetland: 8.02 ng/g, <0.01-85.3 ng/g) which were higher at the WTP, and selenium which was lower at the WTP (WTP median: 5000 ng/g, range: 1950-34,400 ng/g; natural wetland: 19,200 ng/g, 4130-65,200 ng/g). We also measured higher blood o,o'-dityrosine (an indicator of protein damage) at the WTP. No significant differences were found for adult survival, but survival of immature birds at the WTP appeared to be lower which could be due to higher dispersal to other wetlands. Interestingly, we found active avian influenza infections were higher in the natural habitat, while seropositivity was higher in the WTP, seemingly not directly related to pollutant exposure. Overall, we found limited differences in pollutant exposure, health and survival of the shorebirds in the two habitats. Our findings suggest that appropriately managed wastewater treatment wetlands could provide a suitable alternative habitat to these migratory species, which may aid in curbing the decline of shorebird populations from widespread habitat loss.

Bergmann’s and Allen’s rules state that endotherms should be larger and have shorter appendages in cooler climates. However, the drivers of these rules are not clear. Both rules could be explained by adaptation for improved thermoregulation, including plastic responses to temperature in early life. Non-thermal explanations are also plausible as climate impacts other factors that influence size and shape, including starvation risk, predation risk, and foraging ecology. We assess the potential drivers of Bergmann’s and Allen’s rules in 30 shorebird species using extensive field data (>200,000 observations). We show birds in hot, tropical northern Australia have longer bills and smaller bodies than conspecifics in temperate, southern Australia, conforming with both ecogeographical rules. This pattern is consistent across ecologically diverse species, including migratory birds that spend early life in the Arctic. Our findings best support the hypothesis that thermoregulatory adaptation to warm climates drives latitudinal patterns in shorebird size and shape.

Migratory connectivity describes the extent to which migratory species' populations are connected throughout the annual cycle. While recognized as critical for understanding the population dynamics of migratory species and conserving them, empirical evidence of links between migratory connectivity and population dynamics are uncommon. We analyzed associations between spatiotemporal connectivity and differential population trends in a declining and endangered migratory shorebird, the far eastern curlew (Numenius madagascariensis), with multiyear tracking data from across the Australian nonbreeding grounds. We found evidence of temporal and spatial segregation during migration and breeding: curlew from southeast Australia initiated northward migration earlier, arrived at breeding sites earlier, and bred at lower latitudes than curlew from northwest Australia. Analysis of land modification intensity revealed that populations from southeast Australia face greater human impacts compared to those from northwest Australia at both the breeding and nonbreeding grounds, a pattern that aligns with steeper population declines in southeast Australia. This alignment between migratory connectivity, human impacts, and differential population change highlights the importance of a full annual cycle approach to conservation that includes mitigating threats on the breeding grounds and better protecting nonbreeding habitats in Australia where far eastern curlew spend over half of each year.

Light pollution is increasing around the world and altering natural nightscapes with potential ecological and evolutionary consequences. A severe ecological perturbation caused by artificial lights is mass mortalities of organisms, including seabird fledglings that are attracted to lights at night on their first flights to the sea. Here, we report on the number of fledging short-tailed shearwaters Ardenna tenuirostris found grounded in evening and morning rescue patrols conducted at Phillip Island, Australia, during a 15-year period (1999-2013). We assessed factors affecting numbers of grounded birds and mortality including date, moon phase, wind direction and speed, number of visitors and holiday periods. We also tested experimentally if birds were attracted to lights by turning the lights off on a section of the road. Of 8871 fledglings found, 39% were dead or dying. This mortality rate was 4-8 times higher than reported elsewhere for other shearwater species, probably because searching for fledglings was part of our systematic rescue effort rather than the opportunistic rescue used elsewhere. Thus, it suggests that light-induced mortality of seabirds is usually underestimated. We rescued more birds (dead and alive) in peak fledging, moonless and windy nights. Mortality increased through the fledging period, in the mornings and with increased traffic on holiday periods. Turning the road lights off decreased the number of grounded birds (dead and alive). While moon, wind and time are uncontrolled natural constraints, we demonstrated that reduction of light pollution and better traffic management can mitigate artificial light-induced mortality.

Tagging is essential for many types of ecological and behavioural studies, and it is generally assumed that it does not affect the fitness of the individuals being examined. However, the tagging of birds has been shown to have negative effects on some aspects of their lives. Here we investigate the influence of tagging on apparent survival. We examined the effects of flipper bands and injected transponders on the apparent survival of adult Little Penguins by comparing the survival probabilities of 2483 Little Penguins marked at Phillip Island, Australia, between 1995 and 2001 in one of three ways: with bands, with transponders or with both. The design of the study and our method of analysis allowed us to estimate tag loss and ensured that tag loss did not bias the survival estimates. Birds marked with flipper bands had lower survival probabilities than those marked with transponders (with apparent survival probabilities in the first year after tagging of 75% for banded birds and 80% for birds fitted with transponders, and in subsequent years of 87% for banded birds and 91% for birds fitted with transponders). We estimated both band and transponder loss probabilities for the first time, and found that transponder loss probabilities were substantially higher than band loss probabilities, particularly in the first year after marking when the tag loss probability was 5% for transponders and 0.7% for bands. Survival probabilities were lower in the first year after marking than in subsequent years for all birds. Studies of penguins that have used flipper bands to identify individuals may have underestimated annual adult survival probabilities, as banded penguins were likely to have lower than average survival probabilities than those of unbanded birds. The higher annual survival probabilities of individuals marked with transponders indicate that this should be the preferred marking technique for Little Penguins. However, future studies will, like ours, need to consider the higher rates of transponder loss when estimating survival, possibly by double-tagging some birds.