Event Abstract Back to Event Differential effects of the secretome of mesenchymal progenitors from the umbilical cor on the survival and viability of hippocampal, cerebellar and cortical primary cultures of neurons. J. S. Fraga1*, N. A. Silva1, N. M. Neves1, R. L. Reis1, Nuno Sousa1 and A. J. Salgado1 1 University of Minho, Life and Health Sciences Institute (ICVS), School of Health Sciences, Portugal Recent studies have shown that mesenchymal like adult stem cells isolated from umbilical cord’s Wharton Jelly might be used as possible therapeutical agents to treat disorders associated to the central nervous system (CNS). It has been hypothesized that their secretome may play a role on these effects. We have previously shown that these cells produce neuroregulatory factors that modulate the survival of hippocampal neurons. However it is expected that different populations of neurons react differently to the same secretome. Therefore the objective of the present work was to understand the effects of the secretome of a population of mesenchymal progenitor from the umbilical cord’s Wharton Jelly, known as Human Umbilical Cord Perivascular Cells (HUCPVCs), on post-natal neurons isolated from different areas in the brain, namely hippocampus, cerebellum and cortex.HUCPVCs were isolated from the primitive connective tissue present in umbilical cord and further cultured for 3 days at 4000cells/cm2. On day 3 the medium was replaced and 24h, 48h, 72h and 96h thereafter HUCPVCs conditioned media (CM) was collected. The diverse neuronal populations (cortical, cerebellar and hippocampal) were isolated from P4 newborn wistar rats. Upon isolation they were exposed to HUCPVCs CM for 7 days. Control cultures were incubated with standard Neurobasal A medium. Cell viability was assayed by the MTS test while cell proliferation was assessed by a 24h BrdU incubation from days 6 to 7. Cell counts were performed on immunostained cultures for MAP-2 (neurons) and GFAP (astrocytes).For hippocampal neurons CM 96h lead to an increase of the metabolic viability and cell proliferation. Similar effects were also found for cortical neurons. In this particular case CM 96h incubation lead to a six folds increase when compared to controls. Important to mention is that in control cultures no cortical neurons could be observed, while with CM 24h to 72h incubated cultures the total percentage was around 40% of cortical neurons. Concerning cerebellar cultures the highest value of metabolic viability and MAP-2 positive cells was observed with CM 24h whereas CM 48h induced higher levels of cell proliferation. As a conclusion it can be stated that there is a clear evidence that the secretome of HUCPVCs CM leads to different reactions on post- natal neurons from different sources. Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Abstracts Citation: Fraga JS, Silva NA, Neves NM, Reis RL, Sousa N and Salgado AJ (2009). Differential effects of the secretome of mesenchymal progenitors from the umbilical cor on the survival and viability of hippocampal, cerebellar and cortical primary cultures of neurons.. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.086 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Aug 2009; Published Online: 10 Aug 2009. * Correspondence: J. S Fraga, University of Minho, Life and Health Sciences Institute (ICVS), School of Health Sciences, Minho, Portugal, joanafraga@ecsaude.uminho.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado Google J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado Google Scholar J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado PubMed J. S Fraga N. A Silva N. M Neves R. L Reis Nuno Sousa A. J Salgado Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event In vitro and in vivo evaluation on the effects of fitc-labeled cmc/pamam dendrimer nanoparticles on central nervous system derived cells V. H. Pereira1*, S. R. Cerqueira1, J. S. Fraga1, J. F. Mano1, R. L. Reis1, J. M. Oliveira1, Nuno Sousa1 and A. J. Salgado1 1 School of Health Sciences, University of Minho, Life and Health Sciences Research Institute, Portugal Pharmacologic approaches to Central Nervous System (CNS) diseases are a major challenge to neurosciences research. The use of potential new drugs is frequently limited by its difficulty to cross the blood brain barrier, their lack of specificity and the undesirable side-effects associated to it. Carboxymethylchitosan/poly(amidoamine) (CMC/PAMAM) dendrimer nanoparticles are dendrimers-based drug delivery systems with an increased capability to transport drugs coupled with a decreased toxicity. In order to determine the potential applications of these systems to CNS diseases, recent studies characterized the interactions between CMC/PAMAM nanoparticles and in vitro cultures of neurons, astrocytes, microglial cells and oligodendrocytes. The latter revealed good internalization rate and no decrease in cell viability was observed after culturing with CMC/PAMAM nanoparticles at a concentration of 200 Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Abstracts Citation: Pereira VH, Cerqueira SR, Fraga JS, Mano JF, Reis RL, Oliveira JM, Sousa N and Salgado AJ (2009). In vitro and in vivo evaluation on the effects of fitc-labeled cmc/pamam dendrimer nanoparticles on central nervous system derived cells. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.067 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 07 Aug 2009; Published Online: 07 Aug 2009. * Correspondence: V. H Pereira, School of Health Sciences, University of Minho, Life and Health Sciences Research Institute, Minho, Portugal, pereira.vitorhugo@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers V. H Pereira S. R Cerqueira J. S Fraga J. F Mano R. L Reis J. M Oliveira Nuno Sousa A. J Salgado Google V. H Pereira S. R Cerqueira J. S Fraga J. F Mano R. L Reis J. M Oliveira Nuno Sousa A. J Salgado Google Scholar V. H Pereira S. R Cerqueira J. S Fraga J. F Mano R. L Reis J. M Oliveira Nuno Sousa A. J Salgado PubMed V. H Pereira S. R Cerqueira J. S Fraga J. F Mano R. L Reis J. M Oliveira Nuno Sousa A. J Salgado Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

There are no predictive factors for peritoneal dialysis-associated peritonitis; however, its resolution correlates with a cell-mediated Th1 immune response. We tested the hypothesis that induction of receptor-interacting protein 2 (RIP2), an assumed kinase linked with Th1 responses, is a useful marker in this clinical setting. Basal RIP2 expression was measured in human immune cells and during dialysis-associated peritonitis. RIP2 increased with bacterial toxin cell activation and the temporal profile for this differed depending on immune cell involvement in the innate or adaptive phases of the response. Importantly, RIP2 expression increased in peritoneal immune cells during dialysis-associated peritonitis and this upregulation correlated with clinical outcome. An early induction in peritoneal CD14(+) cells correlated with rapid resolution, whereas minimal induction correlated with protracted infection and with catheter loss in 36% of patients. These latter patients had higher levels of MCP-1 consistent with a delayed transition from innate to adaptive immunity. Our study shows that upregulation of RIP2 is a useful marker to monitor dialysis-associated peritonitis and in predicting the clinical outcome of these infections.

Background: Angiocentric cutaneous T-cell lymphomas of childhood (ACTCLC) are an unusual type of T-cell lymphomas that present with a vesiculopapular eruption mimicking hydroa vacciniforme. Most patients have been children from Asia and Latin America.Objective: The purpose of this study was to describe four cases of ACTCLC; to discuss its clinical, histopathologic, and immunohistochemical features; to consider its possible relationship to the Epstein-Barr virus (EBV); and to clarify its classification within the spectrum of angiocentric lymphomas.Methods: The clinical, histopathologic, and immunohistochemical features of four cases of ACTCLC were identified and analyzed. In addition in situ hybridization for EBV was performed in all cases.Results: The clinical features were similar to previous cases reported under different names, such as hydroa-like lymphomas, edematous, scarring vasculitic panniculitis. Histologically, all showed angiocentric infiltrates composed mainly of T cells. In all cases there were variable numbers of CD30(+) cells. The EBV was present in three of the cases.Conclusion: ACTCLC is a distinct type of T-cell lymphoma. It affects mainly children, and the EBV appears to play a role in the pathogenesis of this disease.

Event Abstract Back to Event In Vitro Effects of Human Umbilical Cord Perivascular Cells Conditioned Media on Populations of Dopaminergic Neurons G. Baltazar1, F. Campos1, C. P. Fonseca1, A. C. Cristovão1, J. S. Fraga2*, N. M. Neves3, 4, R. L. Reis3, 4, N. Sousa2 and A. J. Salgado2 1 University of Beira Interior, Research Center in Health Sciences- CICS, Portugal 2 University of Minho, Life and Health Sciences Research Institute (ICVS), School of Health Sciences, Portugal 3 Department of Polymer Engineering, University o Minho, 3B’s Research Group – Biomaterials, Biodegrables and Biomimetics, Portugal 4 IBB- Institute for Biotechnology and Bioengineering, PT Government Associated Laboratory, Portugal Parkinson’s disease (PD) is a neurodegenerative condition in which the populations of dopaminergic neurons are highly affected, leading to consequences that have a major impact on the patients’ life-quality. It has been recently shown that mesenchymal progenitors isolated from the Wharton jelly of the umbilical cord ameliorate the condition of parkisonian rats upon transplantation. It has been hypothesized that these actions are mainly mediated through neurotrophic factors secreted by the cord transplanted cells. However, to our knowledge, this fact is yet to be demonstrated. Therefore, the objective of the present work was the determine the effect of media conditioned obtained from a population of mesenchymal progenitors known as Human Umbilical Cord Perivascular cells (HUCPVCs) on mesencephalic neurons and in particular in the dopaminergic population.HUCPVCs were isolated from the primitive connective tissue present in umbilical cord and further cultured for 3 days at 4000cells/cm2. On day 3 the medium was replaced and 24h, 48h, 72h and 96h thereafter HUCPVCs conditioned media (CM) was collected. This CM was applied onto primary ventral mesencephalic cultures prepared from E15-E16 Wistar rat embryos. Neuronal cells were kept in culture for five days in neurobasal medium supplemented with B27 before being briefly washed and further incubated for 72h with the CM or with medium not subjected to the conditioning process (Control). After this incubation period cells were processed for MAP2 immunocytochemistry and for analysis of tyrosine hydroxylase (TH) expression by Western blot. The effect of the CM on the overall neuronal viability was also examined through the MTT test.Results revealed that CM increased the metabolic viability of the cultures when compared to controls, with a 34% increase in MTT+ reduction for cells treated with 48h CM. Moreover, TH expression was also increased by 45, 55 and 51% in cultures exposed to media conditioned for 24, 48 and 96h, respectively. The protective effect of the CM does not seem to be specific to the dopaminergic neurons since an overall increase on both the number of MAP-2+ cells and TH+ neurons was observed for 24h and 72h CM. Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Abstracts Citation: Baltazar G, Campos F, Fonseca CP, Cristovão AC, Fraga JS, Neves NM, Reis RL, Sousa N and Salgado AJ (1900). In Vitro Effects of Human Umbilical Cord Perivascular Cells Conditioned Media on Populations of Dopaminergic Neurons. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.091 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 Jan 1900; Published Online: 01 Jan 1900. * Correspondence: J. S Fraga, University of Minho, Life and Health Sciences Research Institute (ICVS), School of Health Sciences, Braga, Portugal, joanafraga@ecsaude.uminho.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado Google G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado Google Scholar G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado PubMed G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.