Using improved selection techniques, we isolated four monoclonal antibodies with high affinity for human thyrotropin (Ka = 1.6 X 10(8) to 2.6 X 10(10) L/mol). We used two of these in an immunoradiometric assay (IRMA) that also incorporates a novel phase-separation technology (Sucrosep TSH IRMA, Boots-Celltech). This assay's very low detection limit for TSH (0.03-0.08 milli-int. unit/L) and wide working range (0-250 milli-int. unit/L) allow the differential diagnosis of hypothyroid, euthyroid, and hyperthyroid patients. We compare the utility of this IRMA with that of a RIA for patients with various thyroid disorders. As determined by IRMA, a normal concentration of TSH in serum excludes hyperthyroidism or hypothyroidism, whereas an undetectable serum TSH concentration (less than 0.08 milli-int. unit/L) accurately predicts an abnormality in thyroid gland function.

Conference Abstract| February 01 1983 Effects of Hyper- and Hypothyroidism on Hypothalamic Peptide Concentrations S.G. Daniel; S.G. Daniel 1Department of Medicine, University of Birmingham, Queen Elizaeth Hospital, Birmingham B15 2TH Search for other works by this author on: This Site PubMed Google Scholar K.I.J. Shennan; K.I.J. Shennan 2Department of Medicine, University of Birmingham, Queen Elizaeth Hospital, Birmingham B15 2TH Search for other works by this author on: This Site PubMed Google Scholar M.C. Sheppard; M.C. Sheppard 3Department of Medicine, University of Birmingham, Queen Elizaeth Hospital, Birmingham B15 2TH Search for other works by this author on: This Site PubMed Google Scholar D.B. Ramsden D.B. Ramsden 4Department of Medicine, University of Birmingham, Queen Elizaeth Hospital, Birmingham B15 2TH Search for other works by this author on: This Site PubMed Google Scholar Clin Sci (Lond) (1983) 64 (2): 60P. https://doi.org/10.1042/cs064060Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation S.G. Daniel, K.I.J. Shennan, M.C. Sheppard, D.B. Ramsden; Effects of Hyper- and Hypothyroidism on Hypothalamic Peptide Concentrations. Clin Sci (Lond) 1 February 1983; 64 (2): 60P. doi: https://doi.org/10.1042/cs064060Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsClinical Science Search Advanced Search This content is only available as a PDF. © 1983 The Biochemical Society and the Medical Research Society1983 Article PDF first page preview Close Modal You do not currently have access to this content.

The release of immunoreactive beta-endorphin from rat anterior pituitary cells in culture was studied by radioimmunoassay and gel-filtration chromatography. Two forms of immunoreactivity were detected corresponding to beta-lipotrophin (beta-LPH) and beta-endorphin. Cells were found to release beta-endorphin-like immunoreactivity (beta-endorphin LIR) into the culture medium for up to 10 days with a trough in release occurring between days 2-4. The ratio of beta-LPH to beta-endorphin released remained constant during the course of culture. After 4 days in culture beta-endorphin LIR release was constant and responsive to modulation. Incubation with lysine-vasopressin (0.1 units/ml) produced a two- to threefold increase in release and dexamethasone (10 (-6) mol/l) suppressed release to 33% of controls. Neither stimulation nor suppression resulted in a change in the ratio of beta-LPH to beta-endorphin released. Dexamethasone suppression was not overcome by removal of dexamethasone or addition of vasopressin.

The process of mating of piston rings and cylinder bores in newly assembled Diesel and petrol engines is described, and it is shown that an optimum surface finish exists which leads to rapid running-in—or break-in as it is sometimes termed—when either straight mineral or additive type engine oils are used. In particular it is shown that the running-in of engines fitted with either cast-iron or chromium-plated piston rings may be of short duration when additive type engine lubricants are used in conjunction with a suitable bore finish. Rig and engine tests are described which demonstrate the influence of surface finish on both the wear and the consumption of oil which occur during the running-in process. It is suggested that there are two components of surface finish which affect the running-in process. One of these components, the surface undulations of short wavelength (surface roughness) influences the rate of wear during break-in, whilst the surface undulations of longer wavelength (surface waviness) determine the amount of wear which must occur before the running-in process is complete. Whatever surface finish is chosen for the cylinder bore, to achieve a break-in which is as rapid as possible it is desirable to eliminate those errors in the machining process which give rise to waviness.

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