Significant genetic association exists between rheumatoid arthritis (RA) and cardiovascular disease. The associated mechanisms include common inflammatory mediators, changes in lipoprotein composition and function, immune responses, etc. However, the causality of RA and vascular/heart problems remains unknown. Herein, we performed Mendelian randomization (MR) analysis using a large-scale RA genome-wide association study (GWAS) dataset (462,933 cases and 457,732 controls) and six cardio-cerebrovascular disease GWAS datasets, including age angina (461,880 cases and 447,052 controls), hypertension (461,880 cases and 337,653 controls), age heart attack (10,693 cases and 451,187 controls), abnormalities of heartbeat (461,880 cases and 361,194 controls), stroke (7,055 cases and 454,825 controls), and coronary heart disease (361,194 cases and 351,037 controls) from United Kingdom biobank. We further carried out heterogeneity and sensitivity analyses. We confirmed the causality of RA with age angina (OR = 1.17, 95% CI: 1.04–1.33, p = 1.07E−02), hypertension (OR = 1.45, 95% CI: 1.20–1.75, p = 9.64E−05), age heart attack (OR = 1.15, 95% CI: 1.05–1.26, p = 3.56E−03), abnormalities of heartbeat (OR = 1.07, 95% CI: 1.01–1.12, p = 1.49E−02), stroke (OR = 1.06, 95% CI: 1.01–1.12, p = 2.79E−02), and coronary heart disease (OR = 1.19, 95% CI: 1.01–1.39, p = 3.33E−02), contributing to the understanding of the overlapping genetic mechanisms and therapeutic approaches between RA and cardiovascular disease.

With the continuous development of artificial intelligence (AI) technology, big data-supported AI technology with considerable computer and learning capacity has been applied in diagnosing different types of diseases. This study reviews the application of expert systems, neural networks, and deep learning used by AI technology in disease diagnosis. This paper also gives a glimpse of the intelligent diagnosis and treatment of digestive system diseases, respiratory system diseases, and osteoporosis by AI technology.

Protein methylation is an important and reversible post-translational modification that regulates many biological processes in cells. It occurs mainly on lysine and arginine residues and involves many important biological processes, including transcriptional activity, signal transduction, and the regulation of gene expression. Protein methylation and its regulatory enzymes are related to a variety of human diseases, so improved identification of methylation sites is useful for designing drugs for a variety of related diseases. In this review, we systematically summarize and analyze the tools used for the prediction of protein methylation sites on arginine and lysine residues over the last decade.

Antioxidant proteins play important roles in countering oxidative damage in organisms. Because it is time-consuming and has a high cost, the accurate identification of antioxidant proteins using biological experiments is a challenging task. For these reasons, we proposed a model using machine-learning algorithms that we named AOPs-SVM, which was developed based on sequence features and a support vector machine. Using a testing dataset, we conducted a jackknife cross-validation test with the proposed AOPs-SVM classifier and obtained 0.68 in sensitivity, 0.985 in specificity, 0.942 in average accuracy, 0.741 in MCC, and 0.832 in AUC. This outperformed existing classifiers. The experiment results demonstrate that the AOPs-SVM is an effective classifier and contributes to the research related to antioxidant proteins. A web server was built at http://server.malab.cn/AOPs-SVM/index.jsp to provide open access.

To gain insight into the malfunction of the Golgi apparatus and its relationship to various genetic and neurodegenerative diseases, the identification of sub-Golgi proteins, both cis-Golgi and trans-Golgi proteins, is of great significance. In this study, a state-of-art random forests sub-Golgi protein classifier, rfGPT, was developed. The rfGPT used 2-gap dipeptide and split amino acid composition for the feature vectors and was combined with the synthetic minority over-sampling technique (SMOTE) and an analysis of variance (ANOVA) feature selection method. The rfGPT was trained on a sub-Golgi protein sequence data set (137 sequences), with sequence identity less than 25%. For the optimal rfGPT classifier with 93 features, the accuracy (ACC) was 90.5%; the Matthews correlation coefficient (MCC) was 0.811; the sensitivity (Sn) was 92.6%; and the specificity (Sp) was 88.4%. The independent testing scores for the rfGPT were ACC = 90.6%; MCC = 0.696; Sn = 96.1%; and Sp = 69.2%. Although the independent testing accuracy was 4.4% lower than that for the best reported sub-Golgi classifier trained on a data set with 40% sequence identity (304 sequences), the rfGPT is currently the top sub-Golgi protein predictor utilizing feature vectors without any position-specific scoring matrix and its derivative features. Therefore, the rfGPT is a more practical tool, because no sequence alignment is required with tens of millions of protein sequences. To date, the rfGPT is the Golgi classifier with the best independent testing scores, optimized by training on smaller benchmark data sets. Feature importance analysis proves that the non-polar and aliphatic residues composition, the (aromatic residues) + (non-polar, aliphatic residues) dipeptide and aromatic residues composition between NH2-termial and COOH-terminal of protein sequences are the three top biological features for distinguishing the sub-Golgi proteins.