According to WADA guidelines, the presence of Higenamine (HG) in urine should not be ≥10 ng/mL. HG is widely found in materials used in Chinese herbal medicines as well as food and additives. This paper is the first method wherein a rat model has been used to evaluate the pharmacokinetics of orally administered HG by LC-MS/MS and would be helpful in doping control analysis. The method was found to be linear over a concentration range of 0.5(lower limit of quantification, LLOQ)-500 ng/mL for plasma and 0.5(LLOQ)-1000 ng/mL for urine. The values for intra- and inter-day accuracy and precision did not deviate by >12.25% for HG in plasma and 5.87% in urine. Extraction recoveries of HG were 70.30-86.71% from plasma and 74.93%-79.29% from urine. HG was stable in plasma and urine after the extraction process and when exposed to different storage conditions. The findings of this study could provide some reference value for the assessment of HG misuse and for the control of intake and external application of HG-related materials (foods and medicinal herbs). Our key findings are that high levels of external application or oral administration of HG-rich materials may lead to a positive urine test for HG in athletes.

体育课程是学校基础公共课程之一,但大多数学校会忽略体育课程的重要性,缺乏对体育教学的重视.本文主要对艺术院校体育教学现状进行分析,探索适合艺术院校的体育教学模式.

The determination of higenamine(HG)in Chinese medicinal materials was established by LC-MS/MS in this paper. The separation was performed on Hypersil Gold C18 column(100 mm× 2.1 mm,3 μm)by gradient elution with mobile phase A which was 0.1% formic acid and mobile phase B which was methanol. The flow rate was 0.3 mL/min. Selective reaction monitor(SRM) with the positive ion detection mode was applied to detect the HG by electrospray ionization (ESI)interface. The results were shown that the linear was good in the ranges of 3.05-122 ng/mL for HG (y=1 530.85x-153.164, r=0.999 3). Limit of detection(LOD)and limit of quantification (LOQ)were 0.6 ng/mL and 3 ng/mL respectively. The precision of the within day and between day were 4.08% and 4.73%. The average recovery was 86.98%. HG is found in only 4 of 22 kinds of commonly used Chinese medicinal materials, with content respectively being asarum (8.66 μg/g)>cork(8.216 μg/g)>pepper(5.010 μg/g)>kochia(4.66 μg/g). Finally, the established method of LC-MS/MS is applicable to test HG in Chinese medicinal materials rapidly, sensitively and accurately. It is also suggested that the higenamine is existed in some of Chinese medicinal materials and food materials. Athletes should be caution with those materials.

对于网球运动来说,网球运动员本身不仅要具备较强的技战术能力、力量以及有氧代谢能力,同时也应该具备较强的核心力量水平,这样才能对网球运动中的各类技术要求进行更好地满足.本文主要对核心力量与网球移动步伐的相关问题进行分析.