Bitter compounds in plants, originally evolved as chemical defenses, are retained within the dry matrices of spices and culinary herbs, where they constitute a chemically diverse pool of potential ligands for bitter taste receptors (TAS2Rs) expressed along the gastrointestinal tract. Beyond their role in oral taste perception, TAS2Rs are increasingly recognized as extraoral chemosensory receptors capable of modulating intestinal physiology. Evidence suggests that activation of specific TAS2Rs by bitter phytochemicals can influence glucose transporter activity and stimulate the release of enteroendocrine hormones, including glucagon-like peptide-1 and cholecystokinin, thereby engaging established pathways involved in appetite regulation and glucose homeostasis. These shared downstream effects may explain why structurally diverse bitter phytochemicals often produce convergent metabolic outcomes. Framing bitter-receptor signaling within known enteroendocrine mechanisms highlights an underexplored opportunity to leverage herbs and spices as dietary modulators of metabolic function. Incorporating naturally bitter plant foods into dietary patterns may offer a simple, sustainable, and culturally adaptable approach to support metabolic health.

While a balanced diet can fulfill most nutritional needs, optimizing the composition of specific foods like broccoli can amplify their health benefits. Background/Objectives: Broccoli (Brassica oleracea L. Italica group) is a widely consumed cruciferous vegetable valued for its gastrointestinal and immune health benefits. However, the individual contributions and interactions of broccoli glucosinolates, as they hydrolyze into bioactive isothiocyanates, remain poorly understood. Methods: This study investigated mixtures of four major aliphatic glucosinolates—glucoraphanin, gluconapin, progoitrin, and sinigrin—in individual and combinational models to assess their effects on human colorectal cell proliferation. Results: Combination index analysis revealed moderate to strong antagonistic interactions among these glucosinolates, with the most significant antagonism observed during enzymatic hydrolysis by myrosinase. Mixture analysis identified an optimal glucosinolate ratio including glucoraphanin (81–84%), gluconapin (9–19%), and others (0–7%) to maximize their antiproliferative effects (adjusted R2 > 0.80). This optimal profile was achievable within the target broccoli mapping population. Testing the near-optimal VB067 isogenic broccoli line showed a 44% increase in antiproliferative activity compared to the initial breeding parent or an average sister line. Conclusions: This study highlights the potential of leveraging nutrient–nutrient interactions to guide molecular breeding and produce functional varieties of cruciferous vegetables with optimized health benefits.

The Capsicum genus, native to the Americas and cultivated worldwide for culinary and medicinal purposes, includes five domesticated species with diverse fruit characteristics, pungency, and phytochemical profiles. However, the influence of casual urban backyard growing conditions on these traits remains unknown. In this study, we first assessed morphological production traits of 11 popular pepper cultivars over two growing seasons to establish a consistent baseline for cultivar performance. Next, we evaluated capsaicinoid and phenolic profiles of 47 pepper cultivars, which contribute to their pungency and antioxidant properties. Capsaicinoid profiles revealed species-specific ratios of capsaicin, dihydrocapsaicin, and nordihydrocapsaicin, with C. annuum and C. baccatum displaying an average 64:30:6 profile, C. chinense and C. frutescens showing a capsaicin-dominant 73:25:2 profile, and C. pubescens expressing a distinct dihydrocapsaicin-dominant 34:60:6 profile. Antioxidant activity positively correlated with capsaicinoid content (ABTS: R2 = 0.8264, p < 0.0001; FRAP: R2 = 0.8117, p < 0.0001), with C. chinense (Carolina Reaper) exhibiting the highest activity (FRAP = 111.8 µM TE/g). In LPS-activated macrophages, all cultivars suppressed nitric oxide production both at the enzymatic (66–89%, p < 0.001) and gene expression levels (4.2 to 5.3-fold reduction, p < 0.05). Interleukin IL-1β expression was upregulated (3.8 to 12.9-fold, p < 0.001), while no significant effects were noted on Cox-2, IL-6, and MCP-1 mRNA levels. These results provide novel insights into the molecular and biochemical adaptations of peppers grown in urban environments and underscore the importance of optimizing cultivation conditions to maximize their bioactive potential and health benefits.

Over time, human metabolism evolved to accommodate the challenges and benefits of plant foods that contain high amounts of carbohydrates and polyphenols. The latter are typically metabolized into small phenolic metabolites, including phenolic acids and their endogenous and microbial derivatives, that influence interconnected metabolic pathways involved in nutrient transport, energy metabolism, and neurotransmitter balance. Unlike other natural products, their biological effects arise from weak interactions with multiple molecular pathways rather than a single high-affinity receptor, making them versatile regulators of metabolic health. These compounds also modulate glucose transporters and carbohydrate metabolism, playing a crucial role in postprandial glucose and insulin responses. This review addresses the critical role of phenolic metabolites in metabolic health, with a focus on glucose homeostasis, insulin sensitivity, and carbohydrate metabolism. Incorporating polyphenols and phenolic acids into dietary strategies offers significant potential for improving insulin sensitivity, reducing metabolic disorder risks, and promoting whole-body glucose homeostasis. Furthermore, understanding how phenolic metabolites interact with metabolic pathways is essential for developing future effective nutritional strategies to support metabolic health.

The historical use of oils for beauty and hygiene dates back to ancient civilizations. While mineral oil and its derivatives dominated the personal care industry in the 20th century due to chemical stability and low cost, the environmental impact and sustainability concerns have driven a resurgence in the use of vegetable oils. Cottonseed oil derived from Gossypium hirsutum L. (Malvaceae) has been often overlooked in favor of other plant oils, likely due to cotton’s primary use as a fiber crop. Yet cottonseed oil stands out in cosmetics for its beneficial linoleic to oleic acid ratio, which supports skin barrier function, and its rich profile of phytosterols and tocopherols that provide higher oxidative stability and extended shelf life. Cottonseed oil is also adaptable for use in a variety of formulations, offering a lightweight, non-greasy emollient base with potential applications in skin care, hair, and cleansing products. This review highlights cottonseed oil as a potentially underutilized ingredient in the personal care sector and emphasizes the need for further research and development to fully exploit its properties.

A healthy diet helps transition individuals towards numerous long-term metabolic benefits but is challenging to maintain. Supplementation with additional whole food-based concentrates and herbs may support this shift by enhancing metabolic resilience. This randomized, controlled, crossover study evaluated a 21-day purification program emphasizing plant foods and whole food supplementation on metabolic detoxification, oxidative stress, and gut microbiota composition. Participants followed a plant-based diet for 7 days, with lean proteins introduced from days 8-21. Whole food supplements rich in polyphenols, glucosinolates, and prebiotic fibers were included to support metabolic and gastrointestinal function. The intervention group exhibited 9% (p < 0.0001) reduction in ROS-associated oxidative stress compared to diet alone, suggesting enhanced redox balance. Additionally, the intervention group exhibited higher urinary creatinine levels, indicating enhanced kidney filtration efficiency (p = 0.03). Self-reported stress levels decreased by 36% (p = 0.049) in the intervention group, with a notable reduction in anxiety related to future uncertainty, demonstrating the psychological benefits of dietary supplementation. Gut microbiome analysis revealed a reduction in Proteobacteria, a phylum associated with chronic metabolic and inflammatory diseases, and beneficial increases in Ruminococcus gnavus, Lactobacillus zeae, and Roseburia faecis, which contribute to gut barrier integrity and butyrate production. These findings highlight the potential of whole food-based supplementation to enhance dietary transitions by reducing oxidative stress, supporting kidney function, alleviating psychological stress, and modulating gut microbiota composition. Clinical trial registration:NCT05877365.

Individuals who are overweight or obese are at increased risk of developing prediabetes and type 2 diabetes, yet the direct molecular mechanisms that connect diabetes to obesity are not clear. Chronic, sustained inflammation is considered a strong risk factor in these interactions, directed in part by the short-lived gene expression programs encoding for cytokines and pro-inflammatory mediators. In this study, we show that triptolide administration in the C57BL/6 diet-induced obese mice at up to 10 μg/kg/day for 10 weeks attenuated the development of insulin resistance and diabetes, but not obesity, in these animals. Significant reductions in adipose tissue inflammation and improved insulin sensitivity were observed in the absence of changes in food intake, body weight, body composition, or energy expenditure. Analysis of the core cluster of biomarkers that drives pro-inflammatory responses in the metabolic tissues suggested TNF-α as a critical point that affected the co-development of inflammation and insulin resistance, but also pointed to the putatively protective roles of increased COX-2 and IL-17A signaling in the mediation of these pathophysiological states. Our results show that reduction of diet-induced inflammation confers partial protection against insulin resistance, but not obesity, and suggest the possibility of achieving overweight phenotypes that are accompanied by minimal insulin resistance if inflammation is controlled.

For early hominids, frequent encounters with plant foods necessitated the ability to discern bitter poisons and adjust the activity of the gastrointestinal system in anticipation of carbohydrate-rich meals. Plants bitters were also used historically to manage a variety of metabolic and digestive disorders despite an immense structural diversity of bitter phytochemicals without a common molecular target. Our study confirms these observations in a standardized C57BL/6J prediabetic mouse model using 24 model compounds by demonstrating acute lower peak blood glucose values and improved glucose tolerance following intragastric, but not intraperitoneal, treatment. The administration of the synthetic bitter compound denatonium benzoate yielded similar results that were attenuated by co-application of the allosteric inhibitor of the bitter TAS2R receptors. We also show that these effects occur dose-dependently; associate with reduced glucose uptake, increased intracellular [Ca2+] fluxes, and enhanced GLP-1 expression; and are attenuated by the TAS2R inhibitor in the neuroendocrine STC-1 intestinal cells. These findings support the view that inhibition of glucose transport from the intestinal lumen to the blood by TAS2R bitter receptor signaling in the gut may represent a common mechanism in the acute response to oral ingestion of bitter phytochemicals.

Individually, metabolic variations can significantly influence predisposition to obesity in the form of the obesity-prone (super-responders) and obesity-resistant (non-responders) phenotypes in response to modern calorie-dense diets. In this study, C57BL/6J mice (n = 76) were randomly assigned to either a low-fat diet (LFD) or a high-fat diet (HFD) for 6 weeks, followed by selection of the normally obese (HFD), non-responders (NR), super-responders (SR), or super-responders switched back to the low-fat diet (SR-LFD) for an additional 8 weeks. SR mice showed the highest gains in body weight, lean and fat body mass, and total and free water, in part due to increased feed efficiency, despite having a respiratory exchange ratio (RER) similar to that of NR mice. A switch to the LFD was sufficient to revert most of the observed physiological changes in the SR-LFD mice; however, voluntary physical activity and exercise capacity did not return to the basal level. NR mice showed the highest food intake, lowest feed efficiency, increased oxygen consumption during the light (rest) cycle, increased physical activity during the dark (active) cycle, and increased heat production during both cycles. These variations were observed in the absence of changes in food intake and fecal parameters; however, NR fecal lipid content was lower, and the NR fecal microbiome profile was characterized by reduced abundance of Actinobacteria. Taken together, our findings suggest that NR mice showed an increased ability to metabolize excessive dietary fats in skeletal muscle at the expense of reduced exercise capacity that persisted for the duration of the study. These findings underscore the need for further comprehensive investigations into the mechanisms of obesity resistance, as they hold potential implications for weight-loss strategies in human subjects.

Lameness and restricted mobility are a significant concern in companion animals experiencing chronic pain, inflammation, or age-related pathologies. The growing awareness of health risks and side effects associated with the long-term use of prescription analgesics requires different management strategies to address these issues. In this study, we conducted a crossover evaluation of the effect of full spectrum hemp oil dosed orally at 2 mg/kg BID phytocannabinoids for 8 weeks in dogs (n = 37) living with pain in common household settings. Owner-reported canine pain, home activity, accelerometer-based activity, walkway-based gait, and tolerability were assessed at each phase of the study. Secondary endpoints included changes in blood biochemistry, liver enzymes, inflammatory biomarkers, and plasma metabolites. The intervention was positively associated with a decrease in pain scores (−46.2%, p = 0.0016), increased ability to walk up and down the stairs (10.6–14.7%, p < 0.05), and improved daily activity (25.9%, p = 0.0038). Decreases in plasma levels of proinflammatory cytokines TNF-α, IL-6, and IL-8 were also observed. Taken together, these findings suggest that the benefits of nutritional supplementation with hemp oil could include control of pain, greater mobility, and an overall improvement in the animal wellbeing.

The comfort food (CF) concept emerged during the latter half of the twentieth century. Although not well defined, CF can be described as familiar foods that elicit feelings of well-being and play a role in social interactions and psychological health. These foods are often calorically dense and nutrient-poor, and overconsumption of some CF may contribute to negative metabolic health outcomes. This is particularly relevant when considering the global increase in obesity, leading to the development of therapeutics for improved weight control and metabolic health. In this review, we aim to (a) provide a historical perspective of the CF concept, (b) detail some genetic, developmental, and cultural factors that determine food preference, (c) discuss the influence of diet on the gut-brain connection, hormones, nutrient absorption, and microbiome diversity, and (d) provide a perspective detailing possible future directions in which food technology may enable a new generation of CF with enhanced palatability and nutrient profiles while contributing to well-being and environmental sustainability.

Obesity-resistant (non-responder, NR) phenotypes that exhibit reduced susceptibility to developing obesity despite being exposed to high dietary fat are crucial in exploring the metabolic responses that protect against obesity. Although several efforts have been made to study them in mice and humans, the individual protective mechanisms are poorly understood. In this exploratory study, we used a polygenic C57BL/6J mouse model of diet-induced obesity to show that NR mice developed healthier fat/lean body mass ratios (0.43 ± 0.05) versus the obesity-prone (super-responder, SR) phenotypes (0.69 ± 0.07, p < 0.0001) by upregulating gene expression networks that promote the accumulation of type 2a, fast-twitch, oxidative muscle tissues. This was achieved in part by a metabolic adaptation in the form of blood glucose sparing, thus aggravating glucose tolerance. Resistance to obesity in NR mice was associated with 4.9-fold upregulated mitoferrin 1 (Slc25a37), an essential mitochondrial iron importer. SR mice also showed fecal volatile metabolite signatures of enhanced short-chain fatty acid metabolism, including increases in detrimental methyl formate and ethyl propionate, and these effects were reversed in NR mice. Continued research into obesity-resistant phenotypes can offer valuable insights into the underlying mechanisms of obesity and metabolic health, potentially leading to more personalized and effective approaches for managing weight and related health issues.

Traumatic brain injury (TBI) is one of the leading causes of death worldwide. There are currently no effective treatments for TBI, and trauma survivors suffer from a variety of long-lasting health consequences. With nutritional support recently emerging as a vital step in improving TBI patients' outcomes, we sought to evaluate the potential therapeutic benefits of nutritional supplements derived from bovine thymus gland, which can deliver a variety of nutrients and bioactive molecules. In a rat model of controlled cortical impact (CCI), we determined that animals supplemented with a nuclear fraction of bovine thymus (TNF) display greatly improved performance on beam balance and spatial memory tests following CCI. Using RNA-Seq, we identified an array of signaling pathways that are modulated by TNF supplementation in rat hippocampus, including those involved in the process of autophagy. We further show that bovine thymus-derived extracts contain antigens found in neural tissues and that supplementation of rats with thymus extracts induces production of serum IgG antibodies against neuronal and glial antigens, which may explain the enhanced animal recovery following CCI through possible oral tolerance mechanism. Collectively, our data demonstrate, for the first time, the potency of a nutritional supplement containing nuclear fraction of bovine thymus in enhancing the functional recovery from TBI.

The discovery, progress, and marketing of nutraceuticals and related products are rapidly expanding segments of the food industry which are overtaking the conventional processed food market. The present study investigates the antioxidant, anti-inflammatory, and cellular viability assessment of fennel essential oil. The selection of the extraction process and geographical distribution of fennel play a key role in determining the final composition and hence the corresponding activity of the bio-actives. During the current project, fennel from two origins (Egypt and Pakistan) was procured and subjected to extraction by two methods, i.e., conventional Soxhlet extraction and supercritical fluid extraction, by changing solvent, time, and pressure correspondingly. The resultant extracts were subjected to various analyses, including yield, determination of total phenolic content, and various antioxidant assays. The highest total phenolic content (TPC) was obtained with supercritical fluid extract (206 bar) for Egyptian (180.65 ± 1.22 mg GAE/g) and Pakistani fennel (372.66 ± 0.15 mg GAE/g). The highest values for antioxidant assays were obtained for fennel of Pakistani origin prepared with supercritical fluid extract at 206 bar such as 32.65 ± 0.1 µmole Trolox equivalent/g for FRAP, 74.5

Objectives: In contrast to fast acting simple carbohydrates which cause a quick spike in blood glucose, complex carbohydrates positively influence glycemic response. Research shows combining complex plant-based carbohydrates with proteins further slows digestion, thereby minimizing glucose excursions. As low glycemic index (GI) foods help stabilize postprandial glycemic response, we investigated the impact of Glucose AssistTM, a uniquely designed, whole- food based multicomponent nutritional blend, on post-meal blood glucose response in healthy individuals. Methods: This double blind, crossover randomized controlled trial involved non-diabetic healthy adults (n=10), aged 18-65 years, with BMI ranging from 18.5 to 35 kg/m2. Participants were randomly assigned to consume either rehydratable Glucose Assist or a high glycemic glucose control (TrutolTM) for 2 weeks. Oral glucose tolerance tests (OGTT) were conducted at four visits using continuous glucose monitors (Freestyle Libre Pro), and glycemic index (GI) calculations were used for group comparison. Results: Compared to control, Glucose Assist TM resulted in a lower glycemic response during OGTT without blood glucose peaks/valleys (p=0.003). The novel formulation exhibited a lower GI of 27.4 versus control (GI = 100) as calculated from the Incremental Area Under the Curve (iAUC). Conclusions: Glucose Assist TM is a unique shake which contains ingredients such as pea and pumpkin seed proteins, oat and buckwheat flours, fiber, green banana flour and organic quinoa sprouts. This shake is designed to minimize blood glucose fluctuations, making it a suitable blend for healthy individuals who are seeking low GI options for managing blood glucose levels. Funding Sources: Research sponsored by Standard Process Inc.

Adequate antioxidant supply is essential for maintaining metabolic homeostasis and reducing oxidative stress during detoxification. The emerging evidence suggests that certain classes of phytonutrients can help support the detoxification process by stimulating the liver to produce detoxification enzymes or acting as antioxidants that neutralize the harmful effects of free radicals. This study was designed to examine the effects of a guided 28-day metabolic detoxification program in healthy adults. The participants were randomly assigned to consume a whole food, multi-ingredient supplement (n = 14, education and intervention) or control (n = 18, education and healthy meal) daily for the duration of the trial. The whole food supplement contained 37 g/serving of a proprietary, multicomponent nutritional blend in the form of a rehydratable shake. Program readiness was ensured at baseline using a validated self-perceived wellness score and a blood metabolic panel, indicating stable emotional and physical well-being in both groups. No significant changes or adverse effects were found on physical or emotional health, cellular glutathione (GSH) and the GSH:GSSG ratio, porphyrin, and hepatic detoxification biomarkers in urine. The intervention was positively associated with a 23% increase in superoxide dismutase (p = 0.06) and a 13% increase in glutathione S-transferase (p = 0.003) activities in the blood. This resulted in a 40% increase in the total cellular antioxidant capacity (p = 0.001) and a 13% decrease in reactive oxygen species (p = 0.002) in isolated PBMCs from participants in the detoxification group. Our findings indicate that consuming a whole food nutritional intervention as a part of the guided detoxification program supported phase II detoxification, in part, by promoting enhanced free radical scavenging and maintaining redox homeostasis under the body's natural glutathione recycling capacity.

Obesity is a multifactorial disorder that is remarkably heterogeneous. It presents itself in a variety of phenotypes that can be metabolically unhealthy or healthy, associate with no or multiple metabolic risk factors, gain extreme body weight (super-responders), as well as resist obesity despite the obesogenic environment (non-responders). Progression to obesity is ultimately linked to the overall net energy balance and activity of different metabolic fluxes. This is particularly evident from variations in fatty acids oxidation, metabolic fluxes through the pyruvate-phosphoenolpyruvate-oxaloacetate node, and extracellular accumulation of Krebs cycle metabolites, such as citrate. Patterns of fat accumulation with a focus on visceral and ectopic adipose tissue, microbiome composition, and the immune status of the gastrointestinal tract have emerged as the most promising targets that allow personalization of obesity and warrant further investigations into the critical issue of a wider and long-term weight control. Advances in understanding the biochemistry mechanisms underlying the heterogenous obesity phenotypes are critical to the development of targeted strategies to maintain healthy weight.

Current research has shown that berry-derived polymeric substrates that resist human digestion (dietary fibers and polyphenols) are extensively metabolized in the gastrointestinal tract dominated by microbiota. This review assesses current epidemiological, experimental, and clinical evidence of how berry (strawberry, blueberry, raspberry, blackberry, cranberry, black currant, and grapes) phytochemicals interact with the microbiome and shape health or metabolic risk factor outcomes. There is a growing evidence that the compositional differences among complex carbohydrate fractions and classes of polyphenols define reversible shifts in microbial populations and human metabolome to promote gastrointestinal health. Interventions to prevent gastrointestinal inflammation and improve metabolic outcomes may be achieved with selection of berries that provide distinct polysaccharide substrates for selective multiplication of beneficial microbiota or oligomeric decoys for binding and elimination of the pathogens, as well as phenolic substrates that hold potential to modulate gastrointestinal mucins, reduce luminal oxygen, and release small phenolic metabolites signatures capable of ameliorating inflammatory and metabolic perturbations. These mechanisms may explain many of the differences in microbiota and host gastrointestinal responses associated with increased consumption of berries, and highlight potential opportunities to intentionally shift gut microbiome profiles or to modulate risk factors associated with better nutrition and health outcomes.

Chronic pain is a critical health issue in the US that is routinely managed pharmacologically with diminishing results. The widespread misuse and abuse of prescription opioid pain medications have caused both healthcare providers and patients to seek alternative therapeutic options. Several dietary ingredients have been traditionally used for pain relief and are known to have potential analgesic properties. This double-blind, placebo-controlled randomized clinical trial aimed to test whether a novel combination of full spectrum hemp oil (phytocannabinoids), calamari oil (omega-3 fatty acids), and broccoli (glucosinolates) could reduce chronic pain and attenuate damage from oxidative stress in adults seeking chiropractic care. Participants (average age = 54.8 ± 13.6 years old) were randomly assigned to consume a whole-food, multi-ingredient supplement (n = 12, intervention and standard chiropractic care) or placebo (n = 13, mineral oil and standard chiropractic care) daily for 12 weeks. The subjects' self-reported perceived pain, pain interference, and reactive oxygen species (ROS) status in the peripheral blood mononuclear cells (PBMC) were quantified at baseline, mid-checkpoint, and postintervention. The intervention was positively associated with a 52% decrease in pain intensity and several parameters of pain interference, including quality of sleep. Decreases in the markers of oxidative stress were also observed in the participants from the intervention group (29.4% decrease in PMBC ROS). Our findings indicated that supplementation with a novel combination of hemp oil, calamari oil, and broccoli has the potential to manage chronic pain when combined with standard chiropractic care, as suggested by its effects on pain intensity and oxidative stress.

Mushroom by-products are economical and eco-friendly raw materials with bioactive and functional characteristics that allow for potential uses as food ingredients. However, mushroom upcycling has yet to be fully exploited, despite the many opportunities that mushrooms may offer. The mushroom protein by-product (MPBP) resulting from mushroom protein production was characterized (chemical composition, physicochemical attributes, and functional properties) and incorporated into plant-based batter formulations to prepare four experimental groups with different ratios (w/w, %) of wheat flour (W) to MPBP (100 W, 75 W/25 MPBP, 25 W/75 MPBP, and 100 MPBP). Subsequently, the batter was used for frying batter-coated shrimp, which was evaluated for cooking loss, coating pick-up, oil absorption, and color parameters (L*, a*, and b*). MPBP showed high content of dietary fiber, mainly insoluble fiber (49%), and it is potentially suited for the formulation of high-fiber food products. The MPBP physicochemical attributes pH (11.69), water activity (0.34), L* (58.56), a* (5.61), b* (18.03), and particle size distribution (250–500 µm (22.12%), 125–250 µm (41.18%), 63–125 µm (37.53%), and < 63 µm (0.82%) were noted. Concerning the MPBP functional characteristics, solubility (12.7%), emulsifying activity index (7.6 m2/gr), emulsion stability index (52.4 min), water holding capacity (4.9%), and oil holding capacity (4.8%) were reported. Adding MPBP into batter formulations for batter-coated shrimp resulted in higher values of cooking loss, oil absorption, coating pick-up, and a* color, while lowering L* and b* values. The best experimental results were reported for group 75 W/25 MPBP, which indicates that MPBP can potentially be accepted as a novel batter ingredient for partial substitution of wheat flour.