The endrohel metallofullerene, Gd@C-82, Gd@C-80, Gd-2@C-80 were synthesized by using K-H method. The technique of two-step of high pressure and high temperature extraction with 1, 3, 5-trimethylbenzene (first step) and pyridine(second step) has been successfully utilized to extract metallofullerene of Gd@C-82 The gas-phase negative ions of fullerenes C-n(n= 60, 70, 78, 82, 84...) and metallofullerene (Gd@C-82) have been studied by the ESI-MS, REC-MS and MALDI-TOF-MS. The differences among the mass spectra of ESI-MS, REC-MS and MALDI-TOF-MS have been observed and explained. In contrast to the empty fullerene C-82 , the metallofullerene Gd@C-82 should have a larger HOMO-LUMO gap. Experimental results suggest that Gd@C-82 is polarized and Gd3+ located in the off-center position of C-82 cage after Gd3+ is trapped into C-82 cage.

The gas-phase ion–molecule reactions of C60 with the plasma generated from methyl acrylate under self-chemical ionization conditions were studied by use of a triple-quadrupole mass spectrometer. The adduct cation [C60C3H3O]+ and protonated molecular ion [C60H]+ were observed as the major product ions. The former adduct ion is formed by electrophilic reaction of C60 with the ion [CH2=CHCO]+, a main fragment ion resulting from the methyl acrylate molecular ion [CH2=CHCOOCH3]+ through alpha cleavage. The latter ion is generated by proton transfer from protonated methyl acrylate to C60. Semi-empirical quantum chemical calculations have been performed for the eight possible isomers of [C60C3H3O]+ at the Hartree–Fock level by use of the AM1 method. The results show three types of cycloadducts as the most stable structures among the possible isomers.

The venom and two purified venom enzymes of Agkistrodon blomhoffii Ussuresis have been studied by MALDI - TOF - MS. The relative molecular masses of venom enzymes were determined and their purity tested. Effects of the haunt of snakeS, venom protein concentrations and substrate concen- trations were studied. The results indicate that MALSI - TOF - MS is one of the most effective tools for the assessment of protein purification processes and for the determination of protein molecular masses.