Archaeological, osteological and genetic evidence suggests that Neanderthals lived in small groups1,2; however, less is known about whether these groups were part of isolated communities or belonged to larger, well-connected populations3. The dense concentration of broadly contemporaneous Neanderthal sites in the Meuse Basin, Belgium4, provides a rare opportunity to study regional populations at high resolution. Here we generated genetic data from 27 Neanderthals who lived less than approximately 52,500 years ago from ten archaeological sites in Belgium and France, including a high-coverage genome from a 45,000-year-old individual from Goyet, Belgium. We show that most of these individuals are more closely related to one another than to other contemporaneous late Neanderthals in Europe. Further, some of these individuals carry DNA from a Neanderthal lineage predating the split of late Neanderthals. Although these Neanderthals overlapped temporally with early modern humans in northwestern Europe from around 47,000 years ago, we find no evidence of recent gene flow from modern humans. They also do not show the genetic signatures of mating among close relatives found in Altai Neanderthals, suggesting that they lived in larger or better-connected groups. Moreover, genetic load did not accumulate over time, arguing against progressive genetic deterioration as a driver of Neanderthal extinction.

The human ability to create manufactured substances is not a modern innovation. It began in the Middle Palaeolithic with the production of birch tar through dry distillation, a process of heating bark under low-oxygen conditions. Over time, this technological knowledge extended to other materials and purposes. Among these, cedar products became especially significant, valued in medicine, technology, cosmetics, and mummification. Yet, distinguishing cedar tar from other cedar products, such as resin or essential oil, has long challenged archaeological science due to their closely related chemical composition. Here we used a GC-MS-based metabolomics approach to identify chemical markers of cedar tar samples. Using modern Cedrus atlantica samples, we defined a set of diagnostic compounds (i.e. dihydro-ar-turmerone, cuparene, dihydrocurcumene, ar-himachalene and 1-methyl-3,5-diisopropylbenzene) that reveal both the cedar origin and the transformative process of dry distillation. As a proof of concept, these chemical markers were searched for and successfully detected in ancient Egyptian mummification balms (664-332 BCE), providing direct evidence for cedar tar use in embalming mixtures and new insights into ancient technologies and cultural practices.

ABSTRACT Collagen is a vital archaeological material, preserving biochemical signatures that provide insights into past environments, diets, and human–animal interactions. However, diagenetic processes can lead to rapid and often inconspicuous degradation of collagen. Given the variability in collagen preservation and its significance for analyses such as radiocarbon dating, stable isotope analysis, and ZooMS, researchers have developed various prescreening techniques to assess collagen preservation before destructive sampling. In this study, we applied near‐infrared (NIR) spectroscopy to rapidly and nondestructively prescreen collagen preservation using a previously validated partial least squares regression model. To assess its effectiveness, we analyzed 830 bones from the Cooper's Ferry/Nipéhe site, a dodo and Rodrigues solitaire collection, and a collection of ancient to modern bison. NIR spectroscopy demonstrated high effectiveness in identifying samples suitable for radiocarbon dating, with success rates of 80% (4/5) for Cooper's Ferry/Nipéhe and 91% (10/11) for the dodo and Rodrigues solitaire specimens. Additionally, NIR spectroscopy effectively selected bison samples for ZooMS at a lower collagen yield threshold, with a success rate of 92% (34/37). The results demonstrate that NIR spectroscopy is a viable, nondestructive alternative for collagen prescreening in archaeological bone before destructive analyses, minimizing unnecessary destructive sampling of irreplaceable archaeological materials.

Collagen is a vital archaeological material, preserving biochemical signatures that provide insights into past environments, diets, and human-animal interactions. However, diagenesis can lead to rapid and inconspicuous collagen degradation. Given the variability in collagen preservation and its significance for analyses such as radiocarbon dating, stable isotope analysis, and ZooMS, researchers have developed prescreening techniques to assess collagen preservation before destructive sampling. Current prescreening approaches, including %N and C:N ratios, typically require sample destruction and access to equipped laboratories. Spectroscopic techniques such as Raman spectroscopy and Fourier Transform Infrared spectroscopy have been explored as alternatives, but they are limited in penetration depth, generalizability (at present at least), and are often still destructive, if minimally. Here, we further develop single-point near-infrared (NIR) spectroscopy as a fully non-destructive, rapid, and field-portable method for prescreening bone for collagen preservation. Unlike FTIR and Raman spectroscopic techniques, NIR light penetrates below the surface of bone, enabling assessment of internal collagen preservation without destructive sample preparation. Using Partial Least Squares Regression (PLSR) and Random Forest (RF) modeling, we trained predictive models on whole bones with known collagen yields and validated the models on an independent archaeological collection. Both PLSR and RF models, when restricted to the 2030-2060 nm range, demonstrate strong and comparable performance while avoiding wavelengths associated with consolidants in our reference library. The models outperform traditional % N-based methods in identifying suitable samples for radiocarbon dating. These models enable the high-throughput screening of large collections of bone, improving sample selection and minimizing unnecessary destructive analysis.

The Troisième caverne of Goyet has yielded the largest assemblage of Neandertal remains in Northern Europe with clear evidence of anthropogenic modifications. However, its skeletal fragmentation has long limited detailed morphological and behavioural study on the assemblage. In this study, we integrate palaeogenetic, isotopic, morphometric, and structural analyses of the long bones to assess the biological profiles of the Neandertals from Goyet and explore whether they present particularities that could shed light on the formation of this unique cannibalised assemblage. We identify a minimum of six individuals, including four adult or adolescent females. Compared to Homo sapiens and Neandertals—including regional specimens—the females from Goyet display short statures and reduced diaphyseal robusticity of their long bones. They lack skeletal markers associated with high mobility despite isotopic evidence for non-local origins. The overrepresentation of short, morphologically gracile, non-local females, alongside two immature individuals, suggests a strong selection bias in the individuals present at the site. Dated between 41,000 and 45,000 years ago, a period marked by Neandertal cultural diversity, biological decline and the arrival of Homo sapiens in Northern Europe, the cannibalised female and juvenile Neandertals from Goyet indicate exocannibalism, possibly linked to inter-group conflict, territoriality, and/or specific treatment of outsiders.

The 1998 discovery of a nearly intact Gravettian human burial in the Lapedo Valley (Leiria, Portugal) propelled the Lagar Velho rockshelter to worldwide fame. The ochre-stained skeleton of the Lapedo child, a juvenile aged around four or five, exhibited a mosaic of Neanderthal and anatomically modern human features argued to reflect admixture between the two human populations. Here, we present direct compound-specific radiocarbon dates for the child's skeleton [27,780 to 28,550 calibrated years before present (cal B.P.)] and five associated bones from the burial and underlying contexts. We reassess the chronology and archaeological interpretation of the burial in light of these new dates and demonstrate the suitability of hydroxyproline radiocarbon dating for poorly preserved Paleolithic samples that otherwise fail routine radiocarbon pretreatment methods.

The timing and mechanisms underlying the gradual replacement of Neanderthal populations by Homo sapiens groups have elicited heated debate for decades. The Châtelperronian of France and northern Spain has figured prominently in these discussions. While the Neanderthal authorship of this techno-complex is far less certain than previously thought, the Châtelperronian is now generally accepted as being among the first genuine Upper Paleolithic industries in Eurasia rather than a "transitional" stone tool technology. Here, we report the first association of shell beads with Châtelperronian artifacts from new excavations at the site of La-Roche-à-Pierrot, Saint-Césaire (France), supported by a multiproxy spatial analysis that incorporates site formation processes. Similar types of personal ornaments are unknown from Middle Paleolithic contexts. Comparable examples have, however, been recovered from contexts penecontemporaneous with the Châtelperronian in south-eastern Europe and around the Mediterranean. This hitherto undocumented combination of an early Upper Paleolithic industry and shell beads provides insights into cultural variability in western Europe and raises the question as to whether the makers of the Châtelperronian were influenced by or formed part of the earliest dispersals of H. sapiens into the region.

Neanderthal genomes have been recovered from sites across Eurasia, painting an increasingly complex picture of their populations' structure that mostly indicates that late European Neanderthals belonged to a single metapopulation with no significant evidence of population structure. Here, we report the discovery of a late Neanderthal individual, nicknamed "Thorin," from Grotte Mandrin in Mediterranean France, and his genome. These dentognathic fossils, including a rare example of distomolars, are associated with a rich archeological record of Neanderthal final technological traditions in this region ∼50-42 thousand years ago. Thorin's genome reveals a relatively early divergence of ∼105 ka with other late Neanderthals. Thorin belonged to a population with a small group size that showed no genetic introgression with other known late European Neanderthals, revealing some 50 ka of genetic isolation of his lineage despite them living in neighboring regions. These results have important implications for resolving competing hypotheses about causes of the disappearance of the Neanderthals.

The process by which Palaeolithic Europe was transformed from a Neanderthal-dominated region to one occupied exclusively by Homo sapiens has proven challenging to diagnose. A blurred chronology has made it difficult to determine when Neanderthals disappeared and whether modern humans overlapped with them. Italy is a crucial region because here we can identify not only Late Mousterian industries, assumed to be associated with Neanderthals, but also early Upper Palaeolithic industries linked with the appearance of early H. sapiens, such as the Uluzzian and the Aurignacian. Here, we present a chronometric dataset of 105 new determinations (74 radiocarbon and 31 luminescence ages) from four key southern Italian sites: Cavallo, Castelcivita, Cala, and Oscurusciuto. We built Bayesian-based chronometric models incorporating these results alongside the relative stratigraphic sequences at each site. The results suggest; 1) that the disappearance of Neanderthals probably pre-dated the appearance of early modern humans in the region and; 2) that there was a partial overlap in the chronology of the Uluzzian and Protoaurignacian, suggesting that these industries may have been produced by different human groups in Europe.

•This study presents compound specific dates on bone implements.•Results show a hiatus between the end of the Mousterian and the early Aurignacian.•These are in opposition to admixture patterns observed in Eastern regions.•Population dynamics requires a regional approach based on robust chronometric data.

Summary Neanderthal genomes have been recovered from sites across Eurasia, painting an increasingly complex picture of their populations’ structure, mostly indicating that late European Neanderthals belonged to a single metapopulation with no significant evidence of deep population structure. Here we report the discovery of a late Neanderthal individual, nicknamed “Thorin”, from Grotte Mandrin in Mediterranean France, and his genome. These dentognathic fossils, including a rare example of distomolars, are associated with a rich archeological record of their final technological traditions in this region ∼50-42 thousand years ago. Thorin’s genome reveals a deep divergence with other late Neanderthals. Thorin belonged to a population with small group size that showed no genetic introgression with other known late European Neanderthals, revealing genetic isolation of his lineage despite them living in neighboring regions. These results have important implications for resolving competing hypotheses about causes of the Neanderthals’ disappearance. One Sentence Summary A new French Neanderthal fossil and its genome reveal complex population dynamics during the past 100,000 years.

Ancient Egyptian mummification was practiced for nearly 4000 years as a key feature of some of the most complex mortuary practices documented in the archaeological record. Embalming, the preservation of the body and organs of the deceased for the afterlife, was a central component of the Egyptian mummification process. Here, we combine GC–MS, HT-GC–MS, and LC–MS/MS analyses to examine mummification balms excavated more than a century ago by Howard Carter from Tomb KV42 in the Valley of the Kings. Balm residues were scraped from now empty canopic jars that once contained the mummified organs of the noble lady Senetnay, dating to the 18th dynasty, ca. 1450 BCE. Our analysis revealed balms consisting of beeswax, plant oil, fats, bitumen, Pinaceae resins, a balsamic substance, and dammar or Pistacia tree resin. These are the richest, most complex balms yet identified for this early time period and they shed light on balm ingredients for which there is limited information in Egyptian textual sources. They highlight both the exceptional status of Senetnay and the myriad trade connections of the Egyptians in the 2nd millennium BCE. They further illustrate the excellent preservation possible even for organic remains long removed from their original archaeological context.

ABSTRACT Human remains from the (late) Middle Paleolithic remain rare. Improving our understanding of their spatio-temporal distribution is essential for obtaining insights into human evolution and the dynamics between Neanderthals and early Anatomically Modern Humans (AMHs). We present the single-amino-acid radiocarbon dating and ancient DNA results from the only Neanderthal skeletal remains known in Slovakia (Šal’a I and Šal’a II). As they were found without archaeological context and in secondary deposition, recontextualization is important. By employing the hydroxyproline radiocarbon dating method, we were able to successfully counteract contamination issues and circumvent problems caused by highly degraded collagen. By contrast, DNA analysis did not detect any endogenous DNA at the limits of our resolution. We conclude that the radiocarbon ages of >44,800 BP (OxA-X-2731-16) and >45,100 (OxA-X-2731-15) firmly place the two individuals in the Middle Paleolithic, and before the arrival of AMHs to the region. Furthermore, indirect evidence based on morphology and possibly related faunal remains suggest ages younger than 100 ka. This time frame coincides with a period in which Neanderthal populations were highly dispersed in Europe, yet in decline.

Genomic analyses of Neanderthals have previously provided insights into their population history and relationship to modern humans 1 – 8 , but the social organization of Neanderthal communities remains poorly understood. Here we present genetic data for 13 Neanderthals from two Middle Palaeolithic sites in the Altai Mountains of southern Siberia: 11 from Chagyrskaya Cave 9 , 10 and 2 from Okladnikov Cave 11 —making this one of the largest genetic studies of a Neanderthal population to date. We used hybridization capture to obtain genome-wide nuclear data, as well as mitochondrial and Y-chromosome sequences. Some Chagyrskaya individuals were closely related, including a father–daughter pair and a pair of second-degree relatives, indicating that at least some of the individuals lived at the same time. Up to one-third of these individuals’ genomes had long segments of homozygosity, suggesting that the Chagyrskaya Neanderthals were part of a small community. In addition, the Y-chromosome diversity is an order of magnitude lower than the mitochondrial diversity, a pattern that we found is best explained by female migration between communities. Thus, the genetic data presented here provide a detailed documentation of the social organization of an isolated Neanderthal community at the easternmost extent of their known range.

Kohl, a dark eye cosmetic, is a well-known part of Ancient Egyptian culture. Modern chemical analyses of kohls have largely found lead-based inorganic constituents, whereas earlier studies argued for a much broader range of constituents. Furthermore, organic materials in kohls remain severely understudied. This raises questions regarding the true diversity of materials and recipes used to produce kohls. We analysed the contents of 11 kohl containers from the Petrie Museum collection in London. The objects selected cover a broad range of times and locations in Egypt. Our multi-analytical approach allowed us to characterise both inorganic and organic components. Our data show that inorganic ingredients in kohl recipes are not only lead-based but also manganese- and silicon-based. Our analyses also revealed that organic ingredients derived from both plant and animal sources were commonly used in kohl recipes and sometimes even represent the main constituent. All these findings point towards more varied recipes than initially thought and significantly shift our understanding of Ancient Egyptian kohls.