Background: Mucor infection cannot be ignored in patients with pulmonary shadowing with cavitation. This pa-per reports a case of mucormycosis during the COVID-19 pandemic in Hubei Province, China.Methods: An anesthesiology doctor was initially diagnosed as COVID-19 due to changes in lung imaging. After an-ti-infective, anti-viral, and symptomatic supportive treatment, some of symptoms were relieved. But some symp-toms-'chest pain and discomfort', accompanied by chest sulking and short breath after activities, did not ease. At last, Lichtheimia ramose was detected later by metagenomic next generation sequencing (mNGS) in the bronchoalveolar lavage fluid (BALF).Results: After adjusting amphotericin B for anti-infective treatment, the patient's infection lesions were shrunk and the symptoms were significantly relieved.Conclusions: The diagnosis of invasive fungal infections is very difficult, and mNGS can make an accurate pathogenic diagnosis of invasive fungal diseases for the clinic and provide a basis for clinical treatment.

Objective:To explore the clinical application value of metagenomics next-generation sequencing (mNGS) in assisting the diagnosis of invasive fungal diseases, and to provide pathogenic evidence for the clinical diagnosis and treatment of mucormycosis.Methods:The alveolar lavage fluid of a patient with unexplained lung infection was detected by mNGS to determine the pathogen, and the treatment plan was adjusted according to the mNGS test report.Results:The patient had intermittent chest pain and cough for longer than 5 months. The chest CT showed multiple shadows and cavities in both lungs. There was no abnormality in routine laboratory and pathogenic examinations, and no significant improvement was found after various anti-infection treatments. The mNGS of alveolar lavage fluid submitted for examination revealed that the pathogen was Lichtheimia ramose. After switching to amphotericin B for anti-infection treatment, the patient’s infected lesions shrank and the symptoms relieved significantly.Conclusions:mNGS is an important auxiliary diagnostic method to identify rare pathogens, which is of great significance to assist the diagnosis of lung invasive mycosis.

BackgroundMucor infection cannot be ignored in patients with pulmonary shadowing with cavitation .This paper reports a case of mucormycosis during the COVID-19 pandemic in Hubei Province, China. Case PresentationA anesthesiology doctor was initially diagnosed as COVID-19 due to changes in lung imaging. Later Lichtheimia ramose was found by Metagenomic next generation sequencing (mNGS) in the Bronchoalveolar lavage fluid (BALF).After adjusting amphotericin B for anti-infective treatment, the patient's infection lesions were shranked and the symptoms were significantly relieved. ConclusionThe diagnosis of invasive fungal infections is very difficult, mNGS can make an accurate pathogenic diagnosis of invasive fungal diseases for the clinic and provide a basis for clinical treatment.

Background Recently, COVID-19 is still pandemic in worldwide. India has reported multiple cases of COVID-19 combined with mucormycosis(MM)(1). This paper reports a case during the COVID-19 pandemic in Hubei Province, China. Case Presentation A anesthesiology doctor was initially diagnosed as COVID-19 due to changes in lung imaging. Later Lichtheimia ramose was found by Metagenomic next generation sequencing (mNGS) in the Bronchoalveolar lavage fluid (BALF).After adjusting amphotericin B for anti-infective treatment, the patient's infection lesions were shranked and the symptoms were significantly relieved. Conclusion The diagnosis of invasive fungal infections is very difficult, mNGS can make an accurate pathogenic diagnosis of invasive fungal diseases for the clinic and provide a basis for clinical treatment.