Leymus chinensis, Stipa grandis, Artemisia frigida, and Cleistogenes squarrosa are the dominant plant species in typical temperate grasslands in Xilingol. Intensive studies related to overgrazing, which resulted in a dominant plant shift, have been carried out in recent years, but the ways in which these four species respond to different grazing intensities remain elusive. In this study, the contents of primary metabolites, secondary metabolites, and phytohormones in the leaves of these species under five grazing intensities were assayed and compared. The results showed that A. frigida contained higher amounts of lignin, while C. squarrosa contained higher amounts of total flavonoids than the other species. Leymus chinensis showed a different accumulation of cellulose and tannin in response to grazing, compared with the other three species. Stipa grandis and A. frigida increased in soluble protein contents in response to different grazing treatments. In particular, the contents of phytohormones, such as abscisic acid, salicylic acid, and gibberellins, were markedly changed under grazing. Leymus chinensis exhibited different abscisic acid and gibberellins accumulation patterns compared with the other species, under the different grazing intensities. Patterns of salicylic acid accumulation were similar (except under light and moderate grazing intensities in A. frigida) among the four species. The results indicated that the four species differed in adaptive strategies to cope with the different grazing intensities, and phytohormones played important roles in coordinating the regulation of their growth and grazing tolerance. This study provides a foundation for elucidating the mechanisms of overgrazing-induced degradation of the Xilingol grassland.

Sphingolipids and their intermediates play multiple roles in biological processes. The sphingoid long-chain base component of sphingolipids has emerged as a participant in the regulation of plant biotic and abiotic stress responses. The phytohormone abscisic acid (ABA) regulates many stress responses in plants for environmental adaptation. However, the relationship between the sphingoid bases and ABA is undetermined. In this study, mhp1-1 (the yeast Mpo1 homolog in plants) was isolated through a sodium chloride (NaCl)-sensitivity screen of Arabidopsis transfer DNA (T-DNA) insertion mutants. mhp1-1 was hypersensitivity to salt/osmotic stress and ABA. MHP1 encodes a protein with a domain of unknown function 962 (DUF962). Endoplasmic reticulum-localized MHP1 was found to interact with ABI1. MHP1, a homolog of yeast dioxygenase Mpo1, rescued the growth arrest of mpo1Δ cells caused by ER stress, suggesting functional homology of MHP1 to Mpo1. Overall, MHP1 plays important roles in response to ABA.

Plants are exposed to various environmental cues that lead to reactive oxygen species (ROS) accumulation. ROS production and detoxification are tightly regulated to maintain balance. Although studies of glucose (Glc) are always accompanied by ROS in animals, the role of Glc in respect of ROS in plants is unclear. We isolated gsm2 (Glc-hypersensitive mutant 2), a mutant with a notably chlorotic-cotyledon phenotype. The chloroplast-localized GSM2 was characterized as a transaldolase in the pentose phosphate pathway. With 3% Glc treatment, fewer or no thylakoids were observed in gsm2 cotyledon chloroplasts than in wild-type cotyledon chloroplasts, suggesting that GSM2 is required for chloroplast protection under stress. gsm2 also showed evaluated accumulation of ROS with 3% Glc treatment and was more sensitive to exogenous H2O2 than the wild type. Gene expression analysis of the antioxidant enzymes in gsm2 revealed that chloroplast damage to gsm2 cotyledons results from the accumulation of excessive ROS in response to Glc. Moreover, the addition of diphenyleneiodonium chloride or phenylalanine can rescue Glc-induced chlorosis in gsm2 cotyledons. This work suggests that GSM2 functions to maintain ROS balance in response to Glc during early seedling growth and sheds light on the relationship between Glc, the pentose phosphate pathway and ROS. The chloroplast-localized GSM2 is a transaldolase in the pentose phosphate pathway, which functions in the regulation of glucose-induced ROS to protect chloroplasts from oxidative damage during Arabidopsis early seedling growth.

In plants, sugar acts as an essential signaling molecule that modulates various aspects of metabolism, growth and development, which are also controlled by phytohormones. However, the molecular mechanism of cross-talk between sugar and phytohormones still remains to be elucidated. We have identified gsm1 (glucose-hypersensitive mutant 1) as a mutant with impaired cotyledon development that shows sensitivity to exogenous abscisic acid (ABA). The addition of fluridone can reverse the glucose (Glc) inhibitory effect in gsm1, implying that endogenous ABA is involved in the Glc response of gsm1. In 4.5% Glc, the expression of Glc-induced ABA-responsive genes in gsm1-1 was nearly two times higher than that in the wild type. Compared to gsm1-1, the gsm1-1 abi4-1 double mutant exhibited reduced sensitivity to Glc and ABA, which was similar to the Glc and ABA insensitive phenotype of abi4-1, suggesting that ABI4 is epistatic to GSM1. In the treatment with 4.5% Glc, the GSM1 transcript level was greatly increased in abi4-1 by almost 4-fold of that in the wild type. These data suggest that GSM1 plays an important role in the ABI4-regulated Glc-ABA signaling cascade during Arabidopsis early seedling growth.