Abstract Bletilla striata is highly regarded in traditional Chinese medicine and has also gained widespread attention as a valuable medicinal plant resource. However, the molecular composition and bioactive potential of its polysaccharides present a significant knowledge gap. Herein, a specific glucomannan fraction, designated as BSP60, was purified from Bletilla striata tubers and subsequently subjected to structural, rheological, and biological evaluations. Comprehensive characterization via methylation-GC–MS, monosaccharide composition analysis, and 1D and 2D NMR techniques demonstrated that BSP60 possesses a β-(1 → 4)-linked backbone featuring alternating mannosyl and glucosyl units, with the mannose moieties exhibiting partial O-acetylation at their C-2 or C-3 positions (with an acetylation rate of 23.54%). Rheological studies showed that BSP60 aqueous solutions exhibit shear-thinning behavior and form an elastic-dominated viscoelastic network, with higher viscosity and storage modulus than dextran of comparable molecular weight, indicating enhanced intermolecular interactions. Biological assays revealed that the BSP60 effectively decreased the level of NO, IL-6, and TNF-α in LPS-triggered macrophages (RAW264.7). Furthermore, the scavenge ability of BSP60 to superoxide anion (O2 •−) and ABTS⁺• radicals are significantly higher than that of hyaluronic acid. This study systematically characterizes the structural features of glucomannan derived from Bletilla striata, thereby establishing a robust scientific basis for its expanded utilization in the development of biomedical materials and functional cosmetic ingredients.
Current clinical therapeutic protocols for diabetic foot ulcers (DFUs) remain inadequate due to their low response to therapeutic drugs and high recurrence rates. The normal healing process of diabetic wounds is frequently disrupted by factors such as microbial infections and elevated reactive oxygen species (ROS) levels. In this study, we developed a gel patch that can accelerate wound re epithelialization and scavenge ROS and antibacterial. To provide a dependable biological framework for wound tissue regeneration, this patch incorporates two components analogous to the extracellular matrix: snail glycosaminoglycan and gelatin. The multifunctional patch exhibited potent antibacterial activity, eliminating over 99.9 % of Staphylococcus aureus and Escherichia coli, and reduced reactive oxygen species (ROS) levels in oxidative stress-induced cells by 80 %. In a diabetic wound infection model, the patch inhibited bacterial colonization, accelerated re-epithelialization by two-fold, and lowered inflammatory markers, highlighting its dual antimicrobial and pro-healing effects. The patch demonstrated a precisely synchronized gradual degradation and controlled drug release profile, which aligned with the spatiotemporal dynamics of wound healing progression. In summary, this innovative approach presented a facile, safe, and highly efficient therapeutic strategy for the management of DFUs.
Total synthesis of (±)-applanatumol B has been achieved in in 8 steps from known compound 8 in 5.0% overall yield. The key step features an acid induced cyclization to construct 5/5/6 tricyclic skeleton. Incorporation of dimethyl malonate into the pentane ring simplifies the synthesis by reducing stereocenters to two.
Total synthesis of (& PLUSMN;)-applanatumol B has been achieved in in 8 steps from known compound 8 in 5.0% overall yield. The key step features an acid induced cyclization to construct 5/5/6 tricyclic skeleton. Incorporation of dimethyl malonate into the pentane ring simplifies the synthesis by reducing stereocen-ters to two.& COPY; 2023 Elsevier Ltd. All rights reserved.
Total synthesis of (±)-applanatumol B has been achieved in in 8 steps from known compound 8 in 5.0% overall yield. The key step features an acid induced cyclization to construct 5/5/6 tricyclic skeleton. Incorporation of dimethyl malonate into the pentane ring simplifies the synthesis by reducing stereocenters to two.
