Background and Aims: Identification of noninvasive biomarkers to detect patients with dysmetabolism at risk of nonalcoholic fatty liver disease (NAFLD), severe fibrosis and hepatocellular carcinoma (HCC) is a priority. Progression of NAFLD is genetically conditioned. We previously developed a polygenic risk score (GRS) based on common variants that are involved in inherited predisposition to fat accumulation, namely PNPLA3, TM6SF2, MBOAT7 and GCKR. The aim of this cross-sectional multicenter study was to evaluate the accuracy of GRS to stratify the risk of NAFLD, advanced fibrosis and HCC.

Introduction: Dietary macro-nutrient composition is associated with NAFLD, an inverse correlation between Mediterranean diet and cardiovascular events was reported. Aim to evaluate the role of dietary components on clinical presentation of NAFLD.

Chronic alcohol abuse is a leading cause of cirrhosis, but is also associated with increased cardiovascular mortality. However, scant information is available on the determinants of atherosclerotic and cardiac disease in individuals with alcohol abuse.

Introduction and aims: The natural history of non-alcoholic fatty liver disease (NAFLD) is still not completely defined. We evaluated fibrosis progression rate and possible modulating factors in a cohort of Italian patients with serial biopsy data.

s of the A.I.S.F. Monothematic Conference 2011 / Digestive and Liver Disease 43S (2011), S453–S456 S455

Recently, genome-wide association studies in patients affected by HCV infection have identified a strong association between sustained virological response to peg-interferon/ribavirin and spontaneous viral clearance and common single nucleotide polymorphisms (SNPs) near the IL28B gene, encoding for interferon-lambda-3. Thus, it is anticipated that IL28B genotype determination will be integrated in clinical practice to guide treatment decisions. Here, we describe a simple tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR) for the evaluation of the rs12979860 C>T IL28B SNP, for which strong evidence of association with clinical outcomes has been collected in subjects of European descent. Valid genotypic data were obtained for over 99% of subjects analysed, and T-ARMS-PCR procedures were validated by the analysis of DNA samples of 164 patients with chronic HCV infection. In conclusion, this method allows rapid, reproducible, inexpensive and accurate detection of rs12979860 polymorphism without need of any special equipment and is also suitable for evaluation of a low number of samples on a routine basis.

At 7 weeks, gut microbiota in MCD diet group showed prevalence of Gram+ bacteria with 85% and 15% for Gram-.At 12 weeks this group showed the same prevalence with 99.52% of Gram+ and 0.58% of Gram-.On the opposite, CDHF fed diet showed a prevalence of Gram-at 7 weeks, with 54% and 16.46% of Gram+, while at 12 weeks the group had 80.62% of Gram+ and 19.38% of Gram-.Conclusions: These results showed that CDHF diet induces an increase in Gram-negative bacteria while the MCD diet has the opposite effect in gut microbiota.As it has been shown in humans that an increase in LPS Gram-negative bacteria is probably important in NASH pathogenesis this further reinforces the fact that MCD model in not a good diet model for human NASH.