Abstract Background Transcatheter aortic valve implantation (TAVI) is an effective therapeutic option for severe symptomatic aortic stenosis (AS) with intermediate/high surgical risk. Aim of this study was to examine the acute effect of TAVI in terms of pressure unloading, on left ventricular (LV) mechanics using multilayer global longitudinal strain (GLS) by 2D speckle-tracking echocardiography (ST-E). Methods A total of 44 patients (mean age 81.8 ± 2, 34% male) with severe symptomatic AS and preserved LV ejection fraction (LVEF) underwent 2D echocardiography at baseline and 5 ± 2 days after TAVI. GLS was measured from the endocardial layer (Endo-LS), epicardial layer (Epi-LS) and full thickness of myocardium before and after the procedure. Analysis included other parameters such as age, sex, LV volumes and ejection fraction (LVEF), type of prosthesis implanted, right ventricular (RV) dimension and function. Results By dividing patients in two groups accordingly with LV geometry assessed with regional wall thickness measurement (concentric vs eccentric hypertrophy), better values of Endo-LS were recorded at baseline, in patients with concentric hypertrophy (-12.9 ± 2 vs -11 ± 3, p = 0.048). After TAVI, a significant improvement in Endo-LS was observed, but only in patients with concentric hypertrophy (-12.9 ± 2 vs -14.2 ± 2, p = 0.003). Conclusion The improvement in LS was more prominent in the endocardium, which was evident even immediately after TAVI only in patients with concentric hypertrophy. Evaluation of multilayer strain may provide new insights into the positive effects of unloading in patients with AS and may be potentially useful to predict patients with better outcome after TAVI. Parameter RWT > 0.42 31 pz (70%) RWT ≤ 0.42 13 pz (30%) p Male sex (n, %) 8 (25%) 7 (53%) NS Age (y.o) 81 ± 6 83 ± 7 NS CAD (n, %) 3 (9%) 8 (61%) NS LVEDV (ml) 97 ± 29 134 ± 14 0.002 LVESV (ml) 43 ± 15 72 ± 38 0.001 LVEF(%) 56.2 ± 6 50 ± 12 NS AVA (cm2) 0.8 ± 0.2 0.8 ± 0.3 NS GLS (%) -11.4 ± 3 -10.5 ± 3 NS Endo-LS (%) -12.9 ± 2 -11 ± 3 0.048 Epi-LS (%) -10.8 ± 4 -9.9 ± 3 NS Abstract P1365 Figure.

Abstract Background Cardiovascular disease is a well-recognized cause of increased late morbidity and mortality among survivors of childhood cancer treated with anthracyclines. A decrease in left ventricular (LV) ejection fraction (LVEF) and fractional shortening may be observed during follow-up. Previous studies reported non-negligible prevalence of subclinical systolic dysfunction assessed with deformation imaging at short-, mid- and long-term follow up. Co-administration of Dexrazoxane has been shown to significantly reduce short-term and mid-term cardiotoxicity. The usefulness of dexrazoxane in preventing late (>10 years) anthracycline cardiotoxicity remains under discussion. Purpose Aim of this study was to assess cardiac function in long-term (>10 years) survivors of childhood tumors treated with dexrazoxane/anthracycline association. Methods Twenty cancer survivors previously treated with co-administration of anthracyclines and dexrazoxane for childhood renal tumors or sarcoma and a control group of 20 healthy non-athletic subjects matched for age, sex and body surface area were enrolled in the study. Echocardiographic measurements included 3D LVEF and LV and right ventricular (RV) global longitudinal strain (GLS). Cancer survivors were evaluated at median follow-up time of 21.5 years (range 10-26). Results No evidence of cardiac toxicity, as defined by current guidelines, was reported in all survivors. None of survivors presented LVEF < 50% or abnormal longitudinal strain, defined as a value >2 SDs below the mean using sex-specific and age-specific strain values. No significant differences in standard and deformation imaging parameters were observed between survivors and controls (3D LVEF 58 ± 3 % vs 60 ± 5 % p = NS; LV GLS -21 ± 1 % vs - 21 ± 2 % p= NS; RV GLS - 23 ± 2 % vs - 23 ± 5 % p= NS). Moreover, considering subjects who received a cumulative dose of anthracyclines above the median (doxorubicin-equivalent dose ≥208 mg/m2) no significant differences were found as compared to the group receiving a lower dose. Conclusions No evidence of cardiac toxicity was detected in all survivors. Our findings support the cardio-protective role of dexrazoxane in children undergoing anthracycline-based treatment. Parameters Cancer survivors (n= 20) Controls (n= 20) P 3D LVEF (%) 58 ± 3 60 ± 5 NS LV GLS (%) -21 ± 1 -21 ± 2 NS RV GLS (%) -23 ± 2 -23 ± 5 NS 3 D LVEF: Three-Dimensional Left Ventricular Ejection Fraction, LV GLS: Left Ventricular Global Longitudinal Strain; LV; RV GLS: Right Ventricular Global Longitudinal Strain. Abstract P1781 Figure. LV strain analysis in a survivor

Abstract Background Dobutamine stress echo (DSE) is a useful tool for the evaluation of patients with suspected stable coronary artery disease (CAD). There has been no detailed investigation about the effects of exercise or pharmacological stress on intraventricular fluid dynamics. The possible association between significant CAD and abnormal fluid patterns has not been studied yet. Purpose Aim of the study was to evaluate the intraventricular vortices during dobutamine stress and to find fluid-dynamic patterns associated with the presence of significant CAD. Methods 36 patients scheduled for coronary angiography (CA) and with clinical indication for DSE for suspected CAD were enrolled. Each patient underwent 2D, 3D and contrast echocardiography for Echo-PIV analysis and vortex quantification, both at rest and at peak stress. Vortex geometric and energetic parameters were evaluated using a post- processional software. Intraventricular pressure gradients were evaluated as well. Positive CA for significant CAD was defined as the presence of at least one epicardial coronary stenosis with ≥70% luminal narrowing. Result CA was positive in 58% of patients while DSE in 33%. In the whole population, at peak stress a reduction in vortex area (from 0.36 ± 0.01 to 0.21 ± 0.02; p= 0.001) and in absolute value of vortex intensity (from 0.36 ± 0.1 to 0.26 ± 0.12; p= 0.001) were detected. Vorticity fluctuation and kinetic energy (KE) fluctuation showed a significant increment at peak stress (respectively 0.84 ± 0.17 to 0.93 ± 0.07; p= 0.005; from 1.76 ± 0.37 to 2.47 ± 0.82; p= 0.001), as well as a deviation of flow force momentum angle (φ: from 36 ± 8 to 44 ±9; p= 0.001). Patients with positive CA showed during DSE an higher decrease of the absolute value of vortex intensity (Δ% |vortex intensity| -1.7 ± 0.39 vs -1.3 ± 0.56 vs; p= 0.021), and higher increase of flow force angle (Δ% φ 0.48 ± 0.6 vs 0.1 ± 0.27; p= 0.042). A reversal in the main direction of the vortical flow occurred in 9 patients (25%) at peak stress and 7 of them (64%) were found to have significant right coronary stenosis. This unexpected change in the vortical flow and the presence of right coronary artery stenosis were found significantly associated (X2 p= 0,02). Moreover, patients with circumflex artery stenosis were less likely to have a decrease of vortex length at peak stress (Δ% Vortex length - 0,06 ± 0, 36 vs - 0,34 ± 0,28, p= 0,034). Conclusion Significant changes in intraventricular vortices occur during DSE. The presence of significant CAD evaluated with CA was associated with different behavior of fluid dynamics during DSE. Further studies are needed to assess normal and pathological intraventricular flow patterns evaluated during DSE. Abstract P795 Figure. Vortex reversal at peak stress

Aims: Cardiovascular disease is a well-recognized cause of increased late morbidity and mortality among survivors of childhood cancer treated with anthracyclines. Co-administration of Dexrazoxane has been shown to significantly reduce short-term and mid-term cardiotoxicity. Aim of this study was to assess cardiac function in long-term (>10 years) survivors of childhood tumors treated with dexrazoxane/anthracycline association. Methods and results: Twenty cancer survivors previously treated with co-administration of anthracyclines-dexrazoxane for childhood renal tumors or sarcoma and a control group of 20 healthy subjects were enrolled in the study. Echocardiographic measurements included 3D left ventricular (LV) ejection fraction (LVEF) and LV and right ventricular (RV) global longitudinal strain (GLS). Among cancer survivors group the median age at diagnosis was 5 years (1-17) and they were evaluated at median follow-up time of 21.5 years (10-26). No evidence of cardiac toxicity, as defined by current guidelines, was reported in all survivors. No significant differences in standard and deformation imaging parameters were observed between survivors and controls (3D LVEF 58 +/- 3% vs 60 +/- 5% p = NS; LV GLS -21 +/- 1% vs -21 +/- 2% p = NS; RV GLS -23 +/- 2% vs -23 +/- 5% p = NS). No second tumor was registered in dexrazoxane-treated survivors. Conclusions: Our findings may support the role of dexrazoxane as a useful strategy for cardio-protection in children undergoing anthracycline based treatment. However, large randomized trials are needed to confirm the cardio-protective role of dexrazoxane in pediatric setting at long-term follow-up. (C) 2019 Elsevier B.V. All rights reserved.

AIMS:Left ventricular (LV) diastolic filling is characterized by the formation of a vortex that supports an efficient transit into systolic ejection. Aim of this study was to assess the intraventricular (IV) blood flow dynamics among patients with ST elevated myocardial infarction (STEMI) at different degrees of LV dysfunction, in the attempt to find novel indicators of LV pump efficiency.METHODS AND RESULTS:Sixty-four subjects, 34 consecutive STEMI patients and 30 healthy controls, underwent before hospital discharge 2D speckle tracking echocardiography to assess global longitudinal strain (GLS), and echo-particle image velocimetry analysis to assess flow energetic parameters. Left ventricular volumes ejection fraction (LVEF) and global wall motion score index (GWMSI) were evaluated by 3D echocardiography. ST elevated myocardial infarction patients were subdivided into three groups according to LVEF. Energy dissipation, vorticity fluctuation, and kinetic energy fluctuation indexes, which characterize the degree of disturbance in the flow, exhibit a biphasic behaviour in STEMI patients when compared with controls, with the highest values in patients with still preserved LV function and progressive lower values with LV function worsening. Significant linear correlations were found between energy dissipation index and both LVEF and GLS (r = 0.57, P < 0.001 and r = -0.61, P = 0.001, respectively). Kinetic energy fluctuation index significantly correlates with both LVEF (r = 0.75, P < 0.001) and GLS (-0.58, P = 0.002). Finally, a significant correlation was observed between GWMSI and energy dissipation index (-0.56, P = 0.008).CONCLUSIONS:The present study describes, for the first time, the progression of IV flow energetic properties in patients with acute myocardial infarction at different stages of LV dysfunction when compared with healthy controls. Further data are needed to assess the role of these parameters in the development and maintenance of LV dysfunction.

Global and regional longitudinal strain (GLSRLS) assessed by two-dimensional speckle tracking echocardiography (2D-STE) are considered reliable indexes of left-ventricular (LV) function and myocardial viability in chronic ischaemic patients when compared with delayed-enhanced cardiac magnetic resonance (DE-CMR). In the present study, we tested whether GLS and RLS could also identify early myocardial dysfunction and transmural extent of myocardial scar in patients with acute ST elevation myocardial infarction (STEMI) and relatively preserved LV function. Twenty STEMI patients with LVEF 40, treated with PPCI within 6 h from symptoms onset, underwent DE-CMR and 2D-echocardiography for 2D-STE analysis 6 2 days after STEMI. Wall motion score index (WMSI) and LV ejection fraction (LVEF) were calculated by both methods. Infarct size and transmural extent of necrosis were assessed by CMR. GLS and RLS were obtained by 2D-STE. Mean GLS of the study population was 14 3.3, showing a significant correlation with both LVEF and WMSI, by CMR (r 0.86, P 0.001, and r 0.80, P 0.001, respectively) and time-to-PCI (r 0.66, P 0.038). A weaker correlation was found between GLS and LVEF and WMSI assessed by 2D-echo (r 0.65, P 0.001, and r 0.53, P 0.013, respectively). RLS was significantly lower in DE-segments when compared with normal myocardium (P 0.0001). A cut-off value of RLS of 12.3 by receiver-operating characteristic (ROC) curves identified DE-segments (sensitivity 82, specificity 78), whereas a cut-off value of 11.5 identified transmural extent of DE (sensitivity 75, specificity 78). Our findings indicate that RLS and GLS evaluation provides an accurate assessment of global myocardial function and of the presence of segments with transmural extent of necrosis, with several potential clinical implications.

The intraventricular fluid dynamics is considered a potential novel indicator of cardiac health. This study present an in vivo assessment of the normal left ventricular flow in order to create a reference ground for the assessment of changes in presence of pathology. The systematic analysis is performed by Echographic PIV technique.Normal patients presented small differences in their overall flow features and parameters. The intraventricular vortex flow features a vortex region extending over most of the ventricular length and smoothly accompanies the flow from the inlet toward the outflow tract. The normal intraventricular pressure gradient features a base-to-apex alignment that properly matches with the left ventricular geometry that is adapt to properly sustain forces directed along its axis.The spatial analysis of cardiac flow provides novel information that integrate tissue deformation analysis and improve the understanding of the mechanisms involved in reducing the efficiency of LV pump. (c) 2012 Elsevier Masson SAS. All rights reserved.