ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation of mononitrated polycyclic aromatic hydrocarbons in particulate matter by liquid chromatography and determination by gas chromatography with the thermal energy analyzerBruce A. Tomkins, Roswitha S. Brazell, Mary E. Roth, and Vanessa H. OstrumCite this: Anal. Chem. 1984, 56, 4, 781–786Publication Date (Print):April 1, 1984Publication History Published online1 May 2002Published inissue 1 April 1984https://pubs.acs.org/doi/10.1021/ac00268a044https://doi.org/10.1021/ac00268a044research-articleACS PublicationsRequest reuse permissionsArticle Views74Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

The mutagen, 2-aminonaphthalene, which is the dominant aromatic amine species in several fossil fuels, may be isolated readily by using a sequential high-performance liquid chromatographic procedure. An initial isolation is achieved on a semi-preparative scale aminosilane column and the final separation is completed with a nonpolar stationary phase column on an analytical scale with a pH-adjusted solvent. The use of fluorescence detection permits discrimination (at least 100-fold) against the nonmutagenic isomer, 1-aminonaphthalene. The overall recovery of 2-aminonaphthalene, as determined by scintillation counting of a radioactive 1-aminonaphthalene tracer, typically exceeds 50% and can reach 92% depending on both the sample matrix and the solubility of the isolate in acetonitrile. The minimum detectable quantity is 0.06 μg g-1 of crude sample.

Fluorescamine forms stable, fluorescent derivatives with sterically-unhindered primary aromatic amines in both aqueous (borate buffer, pH 9/acetonitrile) and nonaqueous (pyridine/-acetonitrile) media. Aromatic amines with a substituent such as a methyl group or an aromatic ring ortho to the amine functionality either derivatized poorly or not at all because of steric hindrance. Key Words: FluorescaminePrimary Aromatic AminesFluorescence AnalysisNonaqueous and Aqueous Systems