Floodplains contribute significantly to terrestrial ecosystem service provision but are also among the most vulnerable and degraded ecosystems worldwide. Heterogeneity in floodplain properties arises from variations in river-specific flood regimes, watershed characteristics, and valley morphology, influencing seasonally flooded forests' taxonomic, functional, and phylogenetic diversity. This study addresses persisting knowledge gaps in floodplain ecology, focusing on the seasonally dry tropics. We explore the relationships between flood regime, environmental conditions, vegetation composition, functional and phylogenetic diversity, and the impact of environmental variables on above-ground biomass (AGB) and ecological strategies. The study spans six rivers in southeastern Brazil's main river basins: Rio Grande and São Francisco. We identified five eco-units in each floodplain based on flooding regimes and surveyed six plots per eco-unit. We measured trees with DBH > 5 cm and collected functional traits, along with detailed soil, climate, and water level data. We calculated plot-level floristic composition, taxonomic, functional, and phylogenetic diversity, wood density, and AGB. Functional and phylogenetic dissimilarity were analyzed, and the effects of climate, soil, and hydrological variables were quantified using generalized linear mixed models. We show how flood frequency and duration affect floristic composition across the floodplains. Taxonomic and phylogenetic diversity responded to climate, soil, and hydrological variables, while functional diversity responded primarily to hydrological variables, emphasizing the role of environmental filtering. Hydrological seasonality, soil fertility, and flood regime emerged as key factors shaping community structure and ecological strategies in the studied seasonally flooded tropical forests. Plot-level AGB responded to phosphorus but not to climate or hydrological variables. The study also highlights functional and phylogenetic dissimilarities among eco-units and basins, indicating potential climate change impacts.
Abstract Seasonally Dry Tropical Forests experience pronounced precipitation seasonality, intense solar radiation, and high temperatures, which often translate into high levels of deciduousness during the dry season. In these environments, deciduous species coexist with some evergreen species that are able to maintain their canopy leaves throughout the dry season. To understand the strategies behind this behavior, we analyzed leaf anatomical traits of 13 individuals of Sarcomphalus joazeiro during both the wet and dry seasons in a seasonal deciduous forest. We hypothesized that wet-season leaves would differ anatomically and functionally from dry-season leaves. Specifically, we expected wet-season leaves to show a more acquisitive resource-use strategy compared to dry-season leaves, which we expected to be more conservative. We assessed the effects of season and climatic variables on 25 leaf anatomical traits using generalized linear mixed models (LMM). Leaf traits varied between the wet and dry seasons and interacted with climatic variables, which together suggest that S. joazeiro produces season-specific leaf cohorts. This adaptation allows the species to adjust to the contrasting conditions of light intensity, temperature, and evaporative demand in each season.
Resprouting is a plant persistence strategy in response to disturbance or stressful environmental conditions. Resprouters can dominate in stressful environments such as tropical dry forests (TDFs), but our knowledge of resprouting in TDFs is limited. Here, using a dataset of forest inventories in 16 TDF fragments (covering 15,642 trees and 321 species), we investigated patterns of resprouting in ecosystems subject to substantial seasonal water stress. We focused on two resprouting metrics: the proportion of trees that are multistemmed (resprouting frequency) and the number of stems per tree. In addition, we investigated the relative importance of environmental factors, taxonomic identity, and evolutionary history in resprouting response. Taxa with low to medium resprouting frequencies (17.19%–40.2%) are the most prevalent in TDF, compared to non‐resprouters and high‐frequency resprouters. Overall, resprouting ability appears to be an intrinsic trait that varies in response to environmental conditions but only within a range constrained by taxonomic identity. However, we found no phylogenetic signal above the genus level for any resprouting variables. Thus, the variation in resprouting across TDF lineages likely has been shaped by divergence between closely related taxa and convergence between distantly related ones, reflecting the specific environmental and disturbance factors to which they have been subjected.
Across tropical and subtropical forests and savannas, variation in temperature, precipitation, and edaphic preferences is related to functional characteristics turnover across space. Here we use a unique dataset of 133 woody community sites (127 ha sample and 1351 species included) covering six forest and savanna vegetation types, to reexamine structural and functional patterns and their environmental drivers. Furthermore, we evaluated whether vegetation types (identified by floristic and environmental characteristics) consistently exhibited structural and functional distinctions. We also assessed the importance of vegetation type identity by quantifying the additional contribution of vegetation type in explaining vegetation patterns when compared to environmental models that include temperature, climate, soil and fire. Our variables included fundamental structural characteristics such as number of trees, basal area and average diameter, alongside functional attributes such as aboveground carbon stock, wood density and representativity of multi -stemmed trees. We also include the innovative approach to representativeness of species with compound leaves, which is a proxy for ecological patterns. We found that vegetation types have consistent differentiation for most structural and functional variables, so that vegetation types exist as distinct units beyond floristic differentiation. In addition, environmental variables play an important role in vegetation patterns, but associated with differences between vegetation types for most variables. Overall, vegetation types attributes serve as important drivers of most vegetation variables, contributing up to 30% to the explanation of variable patterns. Our work is the first to explore structural and functional variations among Brazilian tropical and subtropical vegetation types in a broad scale, reviewing ecological patterns previously defined by specific work or carried out using other approaches. Furthermore, our work contributing to the ecological patterns of Brazilian vegetation, in addition to producing reference values that can be used in conservation projects.
Tree growth and longevity trade-offs fundamentally shape the terrestrial carbon balance. Yet, we lack a unified understanding of how such trade-offs vary across the world’s forests. By mapping life history traits for a wide range of species across the Americas, we reveal considerable variation in life expectancies from 10 centimeters in diameter (ranging from 1.3 to 3195 years) and show that the pace of life for trees can be accurately classified into four demographic functional types. We found emergent patterns in the strength of trade-offs between growth and longevity across a temperature gradient. Furthermore, we show that the diversity of life history traits varies predictably across forest biomes, giving rise to a positive relationship between trait diversity and productivity. Our pan-latitudinal assessment provides new insights into the demographic mechanisms that govern the carbon turnover rate across forest biomes.
The tropical forest carbon sink is known to be drought sensitive, but it is unclear which forests are the most vulnerable to extreme events. Forests with hotter and drier baseline conditions may be protected by prior adaptation, or more vulnerable because they operate closer to physiological limits. Here we report that forests in drier South American climates experienced the greatest impacts of the 2015–2016 El Niño, indicating greater vulnerability to extreme temperatures and drought. The long-term, ground-measured tree-by-tree responses of 123 forest plots across tropical South America show that the biomass carbon sink ceased during the event with carbon balance becoming indistinguishable from zero (−0.02 ± 0.37 Mg C ha −1 per year). However, intact tropical South American forests overall were no more sensitive to the extreme 2015–2016 El Niño than to previous less intense events, remaining a key defence against climate change as long as they are protected.
Background: Brazil Nuts (BN) tree is a species of high importance in Amazon region. Their continuous use by traditional communities is often related to disturbances that lead to larger degraded areas where this species is commonly found ("BN groves"). Here we aimed to explore the ecological patterns of BN groves vegetation and its relationship with BN trees and evaluate their potential as a source of carbon credits. We sampled 15 circular plots, with Brazilian Nut trees as the center (focal trees) and collected morphometric data from the focal trees. Additionally, we evaluated fruit production for a period of 5 years to obtain annual measurements, which were used as a proxy of the anthropic impact associated with the collection process. Through analysis of the data, we: i) examined the effects of BN trees on the adjacent vegetation; ii) quantified the potential amount of carbon credits in the adjacent vegetation and in the focal trees by converting carbon stock to equivalent CO2.Results: The adjacent vegetation structure was influenced by the size of BN trees (focal trees). No important effects of BN trees on the adjacent vegetation floristic composition and functional attributes were found. Additionally, we found that Brazilian Nut groves possess a significant potential for carbon credits that could be leveraged in the future carbon credit market,Conclusion: The study highlights the potential for carbon credit generation in Brazil nut groves in the Southeast Amazon as a means of supporting conservation and restoration efforts in these environments.
Tropical montane forests (TMF) of the threatened Atlantic Forest hotspot play an important role in providing essential ecosystem services associated with hydrological regime and biodiversity conservation. However, important ecological patterns such as those related to the woody carbon biogeochemical cycle are not yet known for these forests, especially those located at high elevations (> 1500 m. a.s.l.). Herein, we used a dataset of 60 plots (2.4 ha) of old-growth TMF sampled along a high-elevation gradient (1500-2100 m a.s.l.) and monitored in two inventories (2011 and 2016) to better understand the patterns of carbon stock and uptake of these high-elevation forests and the related environmen-tal (soil) and elevation controls. We found differences in the carbon stock along different elevation levels (120.36-170.4C.ton.ha-1) and a carbon accumulation trend over the period along the entire gradient. Thus, forest car-bon gain (3.82-5.14 ton.ha.year-1) was greater than the carbon loss (2.1-3.4 ton.ha.year-1) and resulted in a positive productivity net. In other words, the TMF acted as a carbon sink, removing carbon from the atmosphere and storing it in woody tissues. Soil variables also exert significant influences on carbon stock and uptake (significative effects of phosphorus on carbon stock and of cation exchange capacity on carbon loss), driving such patterns in isolation or in interaction with elevation. Considering the high conservation degree of the TMF monitored, our results may be indic-ative of a similar trend in other similar forests, but which have gone through disturbances in the more recent past. These TMF fragments have a wide occurrence in the Atlantic Forest hotspot and may also be acting or will soon act as carbon sinks in improved conservation scenarios. Thus, these forests can play an essential role in conserving ecosys-tem services in the region and in mitigating climate changes.
Tropical forests are extremely important for biodiversity maintenance and ecosystem services' provisions. However, such services have been largely threatened by anthropogenic pressures, with a major role from forest fires, particularly in transitional regions that encompass savanna ecosystems. To improve our understanding on the impacts of an anthropogenic fire on the carbon stock and uptake, we used a dataset with 38 tropical forest plots located in a forest-savanna transition region, to test the hypothesis that fire decreases forest's carbon stocks and ceases its ability to sink carbon, becoming a source of carbon for the atmosphere. Tree communities (diameter at the breast height & GE; 5 cm) were monitored for 10 years in the absence of fire (2001 to January 2011), until September 2011, when 24 plots burned (fire from adjacent farms). The two groups of plots (unburned and fire-affected in 2011) were remeasured in 2016, encompassing a 15-year monitoring period over 4 inventories (2001, 2006, 2011 and 2016). We specifically investigated the temporal trends of each group of plots in relation to its carbon stock and uptake and partitioned the contribution of the different component processes (gain, loss, mortality, recruitment, increment and decrement). We found that the trend of increasing forest carbon stock observed in the period before the fire (2001-2011) was abruptly interrupted by fires, decreasing carbon stocks due to negative productivity in the 2011-2016 interval. Carbon losses were mainly driven by an increase in tree mortality, especially in small-DBH trees. In turn, forests that played an important role in carbon sink became a source of carbon to the atmosphere. This result has an important impact on the ecosystem services provided by these forests, especially considering the context of increased fires in the forest-savanna transition regions and its possible interaction with climate change.
Background The mass ratio hypothesis (functional dominance) and niche complementarity hypothesis (functional diversity) are two potential approaches for making the link between biodiversity and biomass. It is yet unclear how biodiversity and biomass are related in seasonally dry tropical forest (SDTF) communities where there is a seasonal water limitation. Aims The objective of this study was to quantify the effects of environmental filtering on ecosystem functioning, especially those related to biodiversity and above-ground biomass. Methods We estimated biomass and functional traits for all species in five plots at five sites in a SDTF. We related functional diversity and community-weighted trait mean (CWM) values to above-ground biomass (AGB) using linear mixed models. Results Functional diversity was not related to AGB, while CWM values of vessel density (VD) were positively and the Carlquist Vulnerability Index (CVI) was negatively related to AGB. Conclusion The CWM values of functional traits related to the trade-off between safety of water transport and the efficiency of water conductivity and conservative strategies (VD and CVI) were good predictors of AGB. The mass ratio hypothesis appears to be a better predictor of AGB than niche complementarity in our study conducted in the SDTF.
Understanding the mechanisms controlling forest carbon storage is crucial to support "nature-based" solutions for climate change mitigation. We used a dataset of 892 Atlantic Forest inventories to assess the direct and indirect effects of environmental conditions, human impacts, tree community proprieties, and sampling methods on tree above-ground carbon stocks. We showed that the widely accepted drivers of carbon stocks, such as climate, soil, topography, and forest fragmentation, have a much smaller role than the forest disturbance history and functional proprieties of the Atlantic Forest. Specifically, within-forest disturbance level was the most important driver, with effect at least 30% higher than any of the environmental conditions individually. Thus, our findings suggest that the conservation of tropical carbon stocks may be dependable on, principally, avoiding forest degradation and that conservation policies focusing only on carbon may fail to protect tropical biodiversity.
Shifts in hydrological regimes alter river flow rates and flood pulses, decrease environmental heterogeneity and the floristic-structural complexity of associated plant communities. We tested the hypothesis that drought events affect plant community composition and structure at a small-scale within a riparian fragment towards a reduction in floristic-structural complexity. The tree community was sampled in three habitats (wet, transitional and dry) and monitored in seven inventories carried out between 1991 and 2018. Hydrological variations were evaluated through annual rainfalls, river flow rates and water level data. The species richness and the detrended correspondence analysis axes were used to characterise the temporal modifications in floristic composition. Community structure was described in terms of biomass: accumulated, growth of survivors, mortality and recruitment. Generalised linear mixed models were fitted to evaluate the effects of time and environment in community. It was concluded that the climate has become drier in recent years due to declining precipitation that has affected flow rates and water levels. The floristic-structural complexity of the study fragment was maintained during the monitoring period. However, prolonged and extreme drought events displayed the potential to impact floristic-structural patterns.
The extent (or lack) of phylogenetic signal for key ecological traits reveals the role of evolutionary processes on present‐day ecosystem function and hints on future ecological trends under climate change scenarios. This approach has been applied to South American tropical moist forests, but not to the highly threatened seasonally dry tropical forests (SDTF), despite the acknowledgement of their unique evolutionary history. To fill this knowledge gap, we investigated the legacy of evolutionary processes on vital ecological characteristics among SDTF trees: regional dominance, tree size and soil preference. We used tree community data on 313 plots of SDTF (12.52 ha) and locally collected soil data in central‐eastern Brazil. For each assessed trait (three for regional dominance, three for tree size and nine for soil preference), we investigated the legacy of evolution using two different approaches: calculating the extent of phylogenetic signal and comparing the fit of four different models of evolution. Above‐ground woody biomass and tree size showed strong phylogenetic signal. Most of the SDTF biomass stock was concentrated on a few large‐sized and closely related tree genera. Among the soil preference variables, only phosphorus displayed significant, albeit weak, phylogenetic signal. Synthesis . Our study is the first to show that evolutionary constraints related to tree size significantly determine regional biomass stocks of seasonally dry tropical forests (SDTF) in a few closely related tree lineages. This suggests that even isolated SDTF fragments with low taxonomic and phylogenetic diversity can play an important role in the global carbon cycle, storing disproportionally large amounts of carbon in trees that belong to high‐biomass lineages. Whether these lineages also share climate change‐induced mortality risk deserves future investigation, as they are largely responsible for the maintenance of regional SDTF biomass stocks.
Forest dynamics are driven by a myriad of factors and link directly to ecosystem services. The dynamics of the highly degraded Brazilian Atlantic Forest (AF) hotspot is mostly driven by its anthropogenic context. Currently, most of the AF region is composed of small disturbed secondary forest fragments that have received partial or full environmental protection with recent environmental norms. Such protection prevents further disturbances and consequently shifts the course of forest dynamics and ecosystem services in AF. Here, we assess long-term forest dynamics trends of a small secondary AF fragment to test the hypothesis that disturbed AF fragments are reaching advanced successional stages. We used a unique dataset of 126 permanent subplots (totaling 5.04 ha) monitored for 30 years (8 inventories between 1987 and 2017) of a disturbed secondary AF fragment (6.35 ha) that has been fully protected since 1986. In each inventory, we measured all living trees with a diameter at breast height (DBH) ? 5 cm and counted survivors, recruits and dead individuals between intervals. We monitored the temporal trends of (i) structural variables (biomass, tree density and species richness), (ii) functional composition (wood density and maximum potential size), (iii) and species dominance. Our results indicate that, now under full protection, the fragment is shifting towards a late successional stage: we observed increasing biomass, decreasing tree density (self-thinning) and increasing abundance of late-successional species with conservative strategies. Species richness and dominant species composition were stable throughout the monitoring period, suggesting an absence of substantial changes in community assembly. These results underscore the role played by small secondary AF fragments in ecosystem service provision (e.g., carbon uptake and shelter for biodiversity) and point to their forest dynamics trends.
Abstract Large‐scale data compilation is increasing steadily in tropical forest research, but the lack of standardized methods for data collection limits drawing inference from large datasets and cross‐biome analyses. Different inclusion methods and minimum tree diameter threshold are among these varying factors. To tackle this issue, we evaluated how different approaches for tree sampling affects our understanding of diversity and functioning in different tropical vegetation types. We used a unique dataset of 44 inventory plots (43.54 ha) encompassing an aridity gradient: evergreen moist forests, semideciduous and deciduous tropical forests. Data were collected using the by‐tree inclusion method, in which, all stems are measured if the equivalent diameter of the tree reaches the minimum threshold. We simulated the impact of adopting different inclusion methods (by‐stem and by‐tree) and different minimum diameter thresholds on the estimation of number of trees and stems, biomass and species richness. We used linear and nonlinear mixed models to investigate the effect of minimum diameter threshold and inclusion method on our different response variables. We also evaluated species chance to be sampled under different minimum inclusion criteria. Inclusion method and minimum diameter threshold mainly affect the estimation of number of trees and stems and species richness, especially in deciduous and semideciduous forests, where resprouting is a prevalent strategy. In these forests, many trees that have several stems do not reach the minimum size individually when adopting the by‐stem method, yet they do reach the minimum size threshold when all stems are considered together. For these environments under water stress, our analysis showed that using large minimum sizes, such as the 10 cm typically used in rainforests, implies large sampling losses, especially when used jointly with the by‐stem inclusion method. The by‐tree inclusion method represents an alternative approach that offers a more reliable sampling in different vegetation types, particularly in those habitats where resprouting is a widely encountered strategy along all age classes. We demonstrate the infeasibility of adopting broad and standard minimum thresholds for different tropical vegetation types, particularly considering their widely different ecological strategies.
Soil is a relevant driver of taxonomic, functional and phylogenetic composition at local scales. However, the mechanisms by which the soil act in these components or how these components interact with each other are not elucidated. Herein, we propose to understand the role of soil (environmental filtering) on the turnover of taxonomic and phylogenetic components and their role in determining the functional composition of Seasonally Dry Tropical Forest (SDTF) communities. We sampled all the arboreal individuals with Diameter at Breast Height equal to or higher than 3 cm and collected soil variables of 25 units from five SDTF fragments located in the Brazilian Caatinga Domain. These data enabled us to obtain the taxonomic, functional and phylogenetic composition of each plot, which were then analyzed by Structural Equation Models jointly with the soil variables. Our results suggest that the soil conditions select specific phylogenetic lineages from a regional species pool according to the adaptive potential, which affects the distribution and abundance of species (taxonomic composition). However, it was not possible to establish a direct relationship between the phylogenetic composition and the taxonomic composition, possibly due to the use of labile functional traits in the analysis. We also found that soil acts directly on the functional composition, leading to a differential success of individuals which would represent a direct and indirect effect of taxonomic composition on the functional composition. Thus, we found community aspects result from different ecological mechanisms which act in a continuous and progressive way between functional, phylogenetic and taxonomic composition.
Aim of study: Aassessing the existence of consistent co-occurrence between tree species that characterize seasonal tropical forests, using the association rules analysis (ARA), that is a novel data mining methodology; and evaluate evaluating the taxonomic and functional similarities between associated species. Area of study: forty-four seasonal forest sites with permanent plots (40.2 ha of total sample) located in Southeast Brazil, from which we obtained species occurrences. Material and methods: we applied association rules analysis (ARA) to the dataset of species occurrence in sites considering the criteria of support equal to or greater than 0.63 and confidence equal to or greater than 0.8 to obtain the first set of associations rules between pairs of species. This set was then submitted to Fisher’s criteria exact p-value less than 0.05, lift equal to or greater than 1.1 and coverage equal to or greater than 0.63. We considered these criteria to be able to select non-random and consistent occurring associations. Main results: We obtained a final result of 238 rules for semideciduous forest and 11 rules for deciduous forests, composed of species characteristic of vegetation types. Co-occurrences are formed mainly by non-confamilial species, which have similar functional characteristics (potential size and wood density). There is a difference in the importance of co-occurrence between forest types, which tends to be less in deciduous forests. Research highlights: The results point to out the feasibility of applying ARA to ecological datasets as a tool for detecting ecological patterns of coexistence between species and the ecosystems functioning. Keywords: data mining; coexistence; semideciduous forests; deciduous forests; biotic interaction.
Forest community dynamics is a topic of great interest in times when the global carbon budget is a widespread concern due to climate change. Among its effects, longer periods of drought and liana proliferation, coupled with land use change, may endanger tropical forest carbon sinks. Here, in a 10.3 ha sampling of six Atlantic semideciduous forests, we investigated the effects of liana crown occupancy and large‐stemmed lianas, as well as their interactions with climate and soil, on forest dynamics. We expected that harsh environmental conditions would enhance the negative effects of lianas on forest productivity. Our hypothesis was corroborated by the findings that the positive effect of lianas on tree mortality increases under drier conditions, as well as their negative effect on tree recruitment. In addition, liana crown occupancy was the best predictor of net aboveground woody biomass productivity, which decreases as liana crown occupancy increases. Our study provides additional evidence of indirect climate change impacts on the tropical forest carbon sink by increasing the negative effects of liana crown occupancy on tree biomass productivity.
Aims We aimed at disentangling the effects of spatial distance, current and past environmental dissimilarity, and their combinations on tree community taxonomic and phylogenetic turnover by addressing the following questions: (i) Is tree community taxonomic and phylogenetic turnover related to the indirect effects of spatial distance via environmental dissimilarity? (ii) Does tree community taxonomic and phylogenetic turnover respond to paleoclimate (Last Glacial Maximum and Mid-Holocene)? Methods The study was carried out in 14 Atlantic rainforest sites in Brazil (20.4 ha sampled) containing 615 tree species from 83 plant families. We obtained plot-level geographic coordinates and soil variables and site-level bioclimatic variables in the current, Mid-Holocene and Last Glacial Maximum. We used structural equation models with a distance-based approach to (i) test the direct effects of spatial distance and environmental dissimilarity and (ii) test the indirect effects of spatial distance via environmental dissimilarity on taxonomic (Bray-Curtis distance) and phylogenetic turnover (Comdist and Comdistnt distances). Important Findings Our results suggest a weak indirect effect of spatial distance via environmental dissimilarity on taxonomic and phylogenetic turnover. Tree community turnover was driven by the direct effects of neutral, niche-based and historical processes. Thus, we inferred that the paleoclimate (historical processes) promoted the selection of the clades that gave rise to the current flora, while spatial distances (neutral processes) limited the dispersal range of species from the regional pool and environmental conditions (niche-based processes) locally selected the taxa that are able to persist.
Resprouting is an ecological strategy widely adopted by trees in response to different restrictive factors, in which the stems can be emitted at different heights of the tree. Although the patterns of resprouting height are already known in response to some restrictive factors (such as fire), their occurrence is not well elucidated in non-fire prone dry environments where continuous resource restriction may stimulate resprouting. Here, we assessed the general ecological dominance of resprouting trees in the community and the ecological dominance of trees resprouting at different heights in response to environmental restrictions in a tropical dry forest (TDF). We used multi-stemmed trees as a proxy for resprouting in response to the local restrictive factors. Our hypotheses were: i) resprouting trees have greater ecological dominance (more trees and stems and higher biomass) in more restrictive plots; ii) the ecological dominance of lower-height resprouting trees is greater in more restrictive plots, while the dominance of upper-height resprouting trees is greater in less restrictive plots. For this, we used a dataset of 27 TDF plots where we measured and identified the trees that met the inclusion criterion (3 cm of diameter at breast height) and collected soil samples to gather data on environmental restriction. Multi-stemmed trees were classified according to the height of additional-stem emission (below-ground, ground-level, and above-ground), considering the bottom-most stem originating from the main stem. We then evaluated whether the ecological dominance of resprouting trees and trees with different resprouting heights varied significantly in response to environmental restrictiveness, and patterns of species composition in the height categories. We found a greater dominance of resprouting trees in more restrictive environments, with lower-height resprouting trees dominating the most restrictive plots (two hypotheses confirmed). This finding may be associated with physiological mechanisms of drought response because resprouting height is directly associated with the level of damage inflicted on the internal structure of trees. We also found that the species varied in their ability for stem emission at different heights, whereby the most plastic species were more dominant compared to those with just one height of emission. This finding indicates that different restrictive factors can produce similar resprouting expressions, reinforcing the need to advance the knowledge on the importance of resprouting in TDF.