It is well known that sera of patients with systemic autoimmunity contain autoantibodies to nuclear antigens. It is also known that patients with systemic autoimmunity have an increased risk for the development of tumours. Interestingly, tumour patients frequently develop autoantibodies and there is a growing list of potential tumour-associated antigens. It is, however, not known whether or not patients with systemic autoimmunity also develop antibodies to tumour-associated antigens. Here we describe the development of a novel multiprotein array allowing us to screen for autoantibodies to 30 different tumour-associated antigens in parallel. Using this novel assay, we found that the frequency of autoantibodies to the selected tumour-associated antigens is increased between 2- and 14-fold in patients with systemic autoimmunity compared with an age-matched control group.

A nonisotopic pool-screening method of point mutations (e.g. SNP) and small insertions / deletions in genetic samples is presented. Based on a formerly described (allele-specific pool-screening, the semi nested PCR is substituted by a one-step method in combination with solid-phase technique and chemiluminometric detection [3].

The aim of this study was to find parameters, that indicate the result of pregnancy or possible diseases the mother could get until the delivery, in amniotic fluid. Thus preventive steps could be made. 344 samples of amniotic fluid were taken in 1996–2001. They were determined through ELISA and RIA and one was able to find the interleukins IL6, IL8, IL15 and IL16, the adhesive molecules sICAM, VCAM, Apo1Fas and also the alpha-fetoprotein, the tumor necrosis factor II receptor, oxalic acid and the tumor marker CEA, CA 14–3, CA 125, CA 19–9 and CA 72–4. For analysing the results, 37 samples were put aside to have a comparison-group. On one side the criterion for this group was an amniocentesis between the 15th and the 16th week of pregnancy and on the other hand no irregularities during the pregnancy, the delivery and the state of the baby after the delivery. All other pregnant women with noticeable differences formed separate groups with different diagnosis. Most of the differences were found within the group of premature births. This group shows significant increase of IL8, sICAM, VCAM and CA 72–4. In the samples of the group that has the fetus in another position than in skull position the sICAM was also increased. VCAM was higher in the group with induced miscarriages because of the chromosomal harm. If the correlation between parameters and the time of taking are put aside, sICAM is much higher with diabetes during pregnancy. Generally, oxalic acid (35,05µmol/l instead of 2,84µmol/l), CA 125 (5941,67 U/ml instead of <65ml) and CA 19–9 (1522 U/ml instead of <30 U/ml) were a lot higher in the samples of the amniotic fluid than the data of the serum of non-pregnant women.

In order to clarify the mechanism of antiatherogenic action of several steroids, we investigated the effects of DHEA-S on the photodioxygenation of human lipoproteins (LDL, HDL). First we found that up to 30% of circulating DHEA-S in humans is located in lipoproteins which were separated by ultracentrifugation. We’ve developed a new chemiluminometric detection method of hydroperoxy-groups in lipoproteins. The separated lipid particles were incubated with several amounts of DHEA-S (5–50µmol/l) and treated for 30min with UV-light in presence of rose bengale and oxygen. The generated reactive singlet oxygen leads in a nonradical pathway to the formation of HOO-groups as primarily lipid peroxidation state. The chemiluminometric measuring signal was directly proportional to the concentration of HOO-groups in the HDL and LDL fractions. We found a drastically decrease of lipid peroxidation state of both HDL and LDL in comparison to not DHEA-S incubated samples. In cases of patients with aortic surgery we found a significant decrease of plasma DHEA-S and increase of androstendione. This effect could be verified by in vitro experiments. It was shown that one of the reaction products of the antioxidative pathway of DHEA-S is androstendione. In conclusion, the protective role of DHEA-S may be caused by inhibition of lipid peroxidation via the singlet oxygen nonradical pathway in vivo.

From extensive research over the last decade it has been known that selenium is essential as necessary component of selenoaminoacids and of specific enzymes. Among others, the redoxpair GSH/GSSG is closely connected with antioxidative processes. Moreover it inhibits and/or activates molecular key reactions with the involvement of various small reactive O- and N-species. We investigated the direct interaction of selenite with components of the respiratory burst of human blood cells, considering the redoxamphoterie of alkali-selenite. Selenite tend to redox-disproportation depending on the pH-value. Whether selenite leads to oxidation or reductation is dependent not only on the pH-value, but also on the redox-potential of the reaction partners. In in-vivo adapted in-vitro conditions (ph = 7.4; mumolar concentrations of reaction partners) we observed the following results: 1. SeO3(2-) is not oxidized by H2O2/NO or triplet-oxygen, when the oxidatives are applied alone; 2. SeO3(2-) is quantitatively oxidized from SeO4(2-) by the combination H2O2/NO2- or O2-/NO; 3. SeO3(2-) is semiquantitatively oxidized by singlett oxygen to SeO4(2-). The composition of reaction products was measured by 77Se-NMR-spectroscopy. The reactive intermediate product for the 2. reaction should be peroxynitrite (HOONO). One cannot rule out the possibility that HOONO reacts on a large scale with H2O2 to singlett oxygen. Subsequently singlett oxygen oxidizes selenite. The pathophysiological impact of singlett oxygen in processes like arteriosclerosis is now being investigated. It has been supposed, that singlett oxygen is participating in processes of lipidperoxidation invivo. Further investigations have to show, to what extent selenite is effective as direct 1O2-scavanger.

Biomedical ChromatographyVolume 13, Issue 2 p. 131-134 Extended Abstract Chemiluminometric measurement of NO and 77Se-NMR studies about the redox-sensitive behaviour of sodium selenite in the presence of peroxynitrite and H2O2/O2 S. Albrecht, Corresponding Author S. Albrecht Department of Gynaecology and Obstetrics, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanyDepartment of Gynaecology and Obstetrics, Technical University of Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany.Search for more papers by this authorT. Zimmermann, T. Zimmermann Department of Visceral, Thoracic and Vascular Surgery, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorD. Scheller, D. Scheller Department of Analytical Chemistry, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorR. Grützmann, R. Grützmann Department of Visceral, Thoracic and Vascular Surgery, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorH. D. Saeger, H. D. Saeger Department of Visceral, Thoracic and Vascular Surgery, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorW. Distler, W. Distler Department of Gynaecology and Obstetrics, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this author S. Albrecht, Corresponding Author S. Albrecht Department of Gynaecology and Obstetrics, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanyDepartment of Gynaecology and Obstetrics, Technical University of Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany.Search for more papers by this authorT. Zimmermann, T. Zimmermann Department of Visceral, Thoracic and Vascular Surgery, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorD. Scheller, D. Scheller Department of Analytical Chemistry, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorR. Grützmann, R. Grützmann Department of Visceral, Thoracic and Vascular Surgery, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorH. D. Saeger, H. D. Saeger Department of Visceral, Thoracic and Vascular Surgery, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorW. Distler, W. Distler Department of Gynaecology and Obstetrics, Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this author First published: 30 April 1999 https://doi.org/10.1002/(SICI)1099-0801(199904)13:2<131::AID-BMC886>3.0.CO;2-2Citations: 2AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume13, Issue2Special Issue: Extended Abstracts from VIIIth International Symposium on Luminescence Spectrometry in Biomedical and Environmental Analysis – Detection Techniques and Applications in Chromatography and Capillary Electropharesis. 26–29 May 1998. Held at University of Las Palmas de Gran Canaria, Canary Islands, SpainApril 1999Pages 131-134 RelatedInformation

Cancer antigen 15-3 (CA 15-3), carcinoembryonic antigen (CEA) and tissue polypeptide antigen (TPA) were measured in 679 sera of breast cancer patients and in 94 sera of women without breast cancer. The tumour markers were determined using immunoluminometric assays (ILMA). The assays are characterised by an inter-assay-imprecision and intra-assay-imprecision <4 %. The breast cancer patients were staged according to the TNM classification stage 0-IV (by UICC) in patient groups with a compatible prognosis. Median and range of each stage were investigated. The cut-off values (95th and 97.5th percentile of control group) of CA 15-3, CEA and TPA were determined; specificity, sensitivity, positive and negative predictive value (PV) and efficiency were investigated for these cut-off's and the receiver operating characteristic (ROC) curves were calculated. The differences between control group and stage 0-3 were shown as non-significant for CA 15-3 and CEA but significant for TPA. Significant differences were found in stage 4 for all three tumour markers. The three tumour markers did not have differences in specificity, positive and negative PV and efficiency. TPA and CA 15-3 demonstrated comparable results in sensitivity and ROC curve analyses. These results were better than those from CEA.

Biomedical ChromatographyVolume 11, Issue 2 p. 77-78 Extended Abstract Interaction of NO, Endothelin and Oxalate in Patients with Systemic Inflammatory Response Syndrome (SIRS) S. Albrecht, Corresponding Author S. Albrecht Department of Gynecology and Obstetrics, The Technical University, Dresden, Ferscherstrasse 74, D-01307, Dresden, GermanyDepartment of Gynecology and Obstetrics, The Technical University, Dresden, Ferscherstrasse 74, D-01307, Dresden, GermanySearch for more papers by this authorT. Zimmermann, T. Zimmermann Department of Surgery, The Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorM. Freidt, M. Freidt Medical Laboratory Bautzen, Töpferstrasse 17, D-02625 Bautzen, GermanySearch for more papers by this authorT. Freidt, T. Freidt Medical Laboratory Bautzen, Töpferstrasse 17, D-02625 Bautzen, GermanySearch for more papers by this authorH.-D. Saeger, H.-D. Saeger Department of Surgery, The Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorW. Distler, W. Distler Department of Gynecology and Obstetrics, The Technical University, Dresden, Ferscherstrasse 74, D-01307, Dresden, GermanySearch for more papers by this author S. Albrecht, Corresponding Author S. Albrecht Department of Gynecology and Obstetrics, The Technical University, Dresden, Ferscherstrasse 74, D-01307, Dresden, GermanyDepartment of Gynecology and Obstetrics, The Technical University, Dresden, Ferscherstrasse 74, D-01307, Dresden, GermanySearch for more papers by this authorT. Zimmermann, T. Zimmermann Department of Surgery, The Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorM. Freidt, M. Freidt Medical Laboratory Bautzen, Töpferstrasse 17, D-02625 Bautzen, GermanySearch for more papers by this authorT. Freidt, T. Freidt Medical Laboratory Bautzen, Töpferstrasse 17, D-02625 Bautzen, GermanySearch for more papers by this authorH.-D. Saeger, H.-D. Saeger Department of Surgery, The Technical University Dresden, Fetscherstrasse 74, D-01307 Dresden, GermanySearch for more papers by this authorW. Distler, W. Distler Department of Gynecology and Obstetrics, The Technical University, Dresden, Ferscherstrasse 74, D-01307, Dresden, GermanySearch for more papers by this author First published: 04 December 1998 https://doi.org/10.1002/(SICI)1099-0801(199703)11:2<77::AID-BMC642>3.0.CO;2-MCitations: 5AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume11, Issue2March 1997Pages 77-78 RelatedInformation