Bryophyllum pinnatum (L. f.) Oken is a kind of herbaceous plant of Crassulaceae, although studies have shown that Bryophyllum pinnatum can treat gastritis, the main active compounds and molecular mechanisms are still unclear. In this study, ultra performance liquid chromatography/mass spectrometry (UPLC-MS) analyses and a strategy of network pharmacology were used to elucidate the potential molecular mechanisms of Bryophyllum pinnatum against gastritis. 85 compounds in Bryophyllum pinnatum were identified through UPLC-MS analysis, 7 main active compounds (quercetin, eicosapentaenoic acid (EPA), isorhamnetin, beta-sitosterol, cubebininolide, kaempferol, artemetin) were obtained based on online database. Kyoto encyclopedia of genes and genomes (KEGG) analysis demonstrated that 93 common targets were mainly enriched in pathways in cancer, PI3K-Akt signaling pathway, FoxO signaling pathway, TNF signaling pathway. AKT1, SRC, EGFR, MAPK1 and MAPK8 targets were the core targets. The molecular mechanisms of Bryophyllum pinnatum to exert anti-gastritis effect were mainly related to the control of cell physiological activities and reduction of stomach acids and gastric proteases. In summary, our study reveals that Bryophyllum pinnatum has the characteristics of multitarget, multi-compound and multi-pathway to exert anti-gastritis effect, which provides the direction and reference for the subsequent in-depth verificationof the mechanisms of anti-gastritis effect of Bryophyllum pinnatum.

为了建立简单、高效的狭苞橐吾组织培养再生体系,本研究以狭苞橐吾的子叶作为外植体,探究不同种类、不同浓度的植物生长调节剂组合对狭苞橐吾离体组织培养的影响.研究结果显示,不同浓度的6-BA与2,4-D组合均能诱导狭苞橐吾子叶产生愈伤组织.当2,4-D浓度为3.0 mg/L、6-BA浓度为1.0、2.0、4.0 mg/L时,愈伤组织诱导率较高,诱导率均在90％ 以上.适宜诱导不定芽的培养基为MS+6-BA 2.0 mg/L+NAA 1.0 mg/L,诱导率为48.00％.在MS+TDZ 1.0 mg/L+NAA 2.0 mg/L培养基中,丛芽的诱导效果较好,诱导率为98.67％.适宜的生根培养基为1/2MS+IAA 0.5 mg/L,其生根率为95.56％,平均生根条数为9.36条,平均长度约为10.48 cm.

Bryophyllum pinnatum (Lam.) Oken is an ornamental and ethno-medicine plant, which can grow a circle of adventitious bud around the leaf margin. The dynamic change of metabolites during the development of B. pinnatum remains poorly understood. Here, leaves from B. pinnatum at four developmental stages were sampled based on morphological characteristics. A non-targeted metabolomics approach was used to evaluate the changes of endogenous metabolites during adventitious bud formation in B. pinnatum. The results showed that differential metabolites were mainly enriched in sphingolipid metabolism, flavone and flavonol biosynthesis, phenylalanine metabolism, and tricarboxylic acid cycle pathway. The metabolites assigned to amino acids, flavonoids, sphingolipids, and the plant hormone jasmonic acid decreased from period Ⅰ to Ⅱ, and then increased from period Ⅲ to Ⅳ with the emergence of adventitious bud (period Ⅲ). While the metabolites related to the tricarboxylic acid cycle showed a trend of first increasing and then decreasing during the four observation periods. Depending on the metabolite changes, leaves may provide conditions similar to in vitro culture for adventitious bud to occur, thus enabling adventitious bud to grow at the leaf edge. Our results provide a basis for illustrating the regulatory mechanisms of adventitious bud in B. pinnatum.

Eucommia ulmoides Oliver is a precious economic crop, which can be used as raw industrial materials and traditional Chinese medicine. Secondary metabolites may vary in type, content, and proportion due to different origins and cultivars, and are closely related to the intrinsic quality of the medicinal plant. In this study, a metabolomics method was applied to investigate the effects of regions and cultivars on the changes of endogenous metabolites in samaras of ‘Qinzhong’ and ‘Huazhong’, two cultivars of E. ulmoides. The differential metabolites mainly enriched in pathways related to flavonoids when the same cultivar was cultivated in different regions. The differential metabolites between the two cultivars were primarily concentrated in flavonoids, sphingolipids, and glutathione. Therefore, it is speculated that flavonoids play an essential role in the adaption of the environment and formation of different cultivars. The research provides a direction for the rational utilization of E. ulmoides samaras.

Anaphalis hancockii Maxim is often used as an aromatic plant in China. To effectively protect and utilize the resources of A. hancockii, a completely rapid micropropagation system has been developed. Additionally, ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS) technology, coupled with multivariate statistical analysis and pathway enrichment analysis, was used to assess the regulatory influences of 6-benzylaminopurine (6-BA) and kinetin (KT) applied in A. hancockii. The results showed that when MS medium was supplemented with 4.0 mg/L 2,4-D and 2.0 mg/L KT, the effect of inducing callus from leaf, petiole, and root explants was the best. At this time, the leaf callus induction rate was 94.67