With the increase of global cancer morbidity and mortality, it is of great importance to control the development of cancer. In recent years, plenty of strategies have been developed for tumor diagnosis and therapy. As one of the major approaches, ligand-mediated receptor-introduced drug delivery has been extensively studied over the past decade. Our laboratory mainly engages in the related researches on the combined diagnosis and treatment of tumors, especially glioma. A series of smart biocompatible theranostic systems were designed and constructed, including precise oligomer-based nanoparticles, polymer-coated nitrogen carbon nanodots, multifunctional mesoporous silica graphite nanosheets, magnetic graphene mesoporous silica nanoparticles, etc. Then, based on the design of specific ligand-receptor targeting strategies such as IL-13, Angiopep and I6P7, these systems were applied for tumor-targeted drug delivery, gene therapy, photothermal therapy and tumor imaging. For example, the nuclear localization signal sequence LNP in Lim kinase 2 protein, was used to modify the dendrimer-based nano gene delivery system. With the ability to cross the blood-brain barrier, the translocation efficiency of nanoparticles and anti-glioma effect were improved. And, the new controlled drug delivery system, using graphene as nanocarrier, was prepared with excellent properties, such as pHand NIRresponsive, and controlled drug release, showing chemotherapeutic and photothermal synergistic therapy of glioma. A receptor-mediated cascade-targeting drug delivery system, which constructed by polymer-coated nitrogen-carbon nanodots and a multifunctional peptide (I6P7) with the ability to transport through the blood-brain barrier, target glioma and inhibit the expression of tumor growth factors, were constructed for the imaging-guided combined glioma therapy. Relating work provides new ideas and theoretical basis for cancer theragnosis.

Omega-6 fatty acid desaturase ( FAD2) is a critical enzyme in linoleic acid biosynthesis , and it converts oleic acid to linoleic acid by desaturation .In this study, an omega-6 fatty acid desaturase was obtained from Paeonia delavayi, which shared 98.7% identities with that of Peaonia lactiflora, and named PdFAD2.Homology analysis showed that deduced PdFAD2 protein has three conserved histidine motifs.Expression profiling analysis revealed that PdFAD2 was expressed strongly in flower, stem, leaf and seed but slightly expressed in root.This result will pro-vide basic information for unsaturated fatty acid biosynthesis research and molecular breeding in Paeonia delavayi.

非核糖体肽具抗生素、抗病毒、抗癌及免疫抑制剂等重要的药用价值.以长松萝的地衣型真菌为研究材料,通过同源克隆和Gene walking的方法获取长松萝地衣型真菌全长非核糖体肽合成酶基因(UsNRPS),并对得到的UsNRPS进行生物信息学分析、分子系统进化分析及基因表达分析.结果表明:在地衣型真菌中,非核糖体肽在长松萝中为首次发现,BLAST比对表明其为NRPS基因的A结构域,生物信息学分析显示UsNRPS蛋白是一种非分泌蛋白,位于细胞质基质中;分子进化分析显示UsNRPS与埃默森蓝状菌(Rasamsonia emersonii)的NRPS基因的A结构域蛋白聚为一类;RT-PCR分析表明:2％肌醇、10％甘露醇、2％山梨醇、2％葡萄糖、10％葡萄糖、0.2％谷氨酰胺、1％甘氨酸、0.2％丙氨酸能够诱导NRPS基因的轻微表达,2％、10％蔗糖和果糖均能诱导NRPS基因的强烈表达;0.2％、1％的天冬氨酸均抑制NRPS基因的诱导表达.

In order to studying the effect of different carbon sources and nitrogen sources on mycelia growth of Ant-rodia cinnamomea, 9 kinds of carbon sources and nitrogen sources were selected and control variable method were used to prepare the medium. The growth of mycelia on different carbon sources, different nitrogen sources and dif-ferent concentrations of fructose were observed. The result showed that using yeast extract as nitrogen source and u-sing maltose and fructose as carbon source can promote the growth of mycelium rapidly. The growth rate reached1. 47mm/d after 30 d’ s culturing, and this medium can make the color of mycelium change to be red. The different nitrogen sources experiments showed, using beef extract powder as a nitrogen source can significantly promote the growth of A. cinnamomea. The growth rate reached 1. 51mm/d after 30 d’s culturing, and the color of mycelium was white. When the concentrate of fructose were within 4 %, all relevant indicators increased with the increasing concentrate. This study provide a groundwork for expand breeding culture and large-scale fermentation of A. cinnamomea, and lay an important theoretical basis to inoculate on the basswood.