Three strains with chitinase activity were isolated from soil.The strain with the maximum enzyme activity level was a Gram negative bacterium which was identified as Stenotrophomonas maltophilia chitinase strain by 16S rDNA method.The study on the synthetic conditions for the chitinase by single factor optimization method and uniform design method showed that the optimal synthesis conditions for chitinase was as follows: the strain was cultured for 60 hours in a basic medium(pH 7.2,30 ℃) added by 0.5% chitin and 1.0% peptone.

With the method of Bioinformatics,some essentiality characters of chitinase C(Chi C)gene in Streptomyces coelicolor were analysed,and made a cladogram from some gene of chitinase in Streptomyces sp.From the analyse date we validated that there are at lest eight species chitinases in Streptomyces coelicolor.Meanwhile the struction of the chitinase c(Chi c) in Streptomyces coelicolor was forecasted,the senior structure illustrative pictures of the protein,which code by the gene and belong to 18 family,also were forecasted.

Chitinase gene ChiC from Serratia macescens ATCC14041 was cloned.The PCR product was inserted into the pMD18-T Vector(named pMD-ChiC).The recombinant plasmid was transformed into E.coli DH5α.By # sequenced and cleaved with Bam HI/Nhe I,it shows that recombinant plasmid contains ChiC.Bioinformatics analysis shows that the length of complete coding sequence was 1 443 bp,and the protein of the ChiC had a 480-residue precursor.The deduced isoelectric point was 5.63,and the molecular weight was about 52 kD.Chitinase cladogram of Serratia sp.was made,and the classification of Chitinase C in Serratia sp.was validated.Meanwhile the tertiary structure sketch map of the chitinase ChiC from S.marcescens was forecasted,and the senior structure sketch of the protein which belongs to 18 family was made.

The active calcium oxide was made from seashell by crushing, sieving and two times' high temperature incinerating(1100℃) and so on.Using calcium-indicator,the average purity of the active calcium oxide was meansurated with the results reached to 97%,and its white chroma was 92% which was more than the white-chroma of common calcium oxide.Furthermore,its heat temperature in water was only 40℃ which is less than the heat temperature of common calcium oxide(60℃).Comparing by NaClO and common calcium oxide,the inhibiting bacterium experiments have been done with cucumber and urechis unicinctus.After 48 hours, the bacterium inhibiting rate of 0.5% active calcium oxide could reach 94.4% for cucumber and about 97.5% for urechis unicinctus﹔while the 0.5%common calcium oxide inhibiting rate is about 91.7% for cucumber and 81.5% for urechis unicinctus.The result of the experiment showed the active calcium oxide has evident function in bacterium inhibition.