[Background] Brown rot on peach and nectarine trees caused by Monilinia spp. is an important fungal disease, and produces serious economic losses frequently in China. There may exist more than one species of peach brown rot pathogens. [Objective] To clarify the species and pathogenicity of brown rot pathogens from different peach-growing areas in China. [Methods] The pathogens were isolated from peach brown rot fruits of different peach production areas, using the method of single spore isolation. Identification and analysis of isolates were carried out by morphological characteristics, molecular biology methods, and inoculation assay. [Results] In this study 15 brown rot pathogen isolates were obtained from 8 different peach-growing areas. The morphological characteristics of the isolates were slightly different from each other, but the isolate hfyn from Yunnan Province was significantly different from the other isolates. Based on its morphology, rDNA ITS sequence and phylogenetic analysis, the isolate hfyn was identified as Monilia yunnanensis, which was a newly found brown rot pathogen, and the other 14 isolates were identified as Monilinia fructicola. PCR amplification using several pairs of specific primers confirmed the morphological and rDNA ITS identification results. Inoculation tests showed that the pathogenicities on peach fruits of 15 isolates from different peach producing areas were different. The isolates from Lishui, Zhejiang Province, Shijiazhuang, Hebei Province, and Qingdao, Shandong Province had more virulent, and the virulences of isolates from Kunming, Yunnan Province and Tai'an, Shandong Province were relatively weak. [Conclusion] The results indicated that the major pathogen of brown rot in peach-growing areas in China was Monilinia fructicola, and there were differences in morphology and pathogenicity among these isolates from different peach-growing areas. The result from the present study may provide a scientific basis for managing peach brown rot epidemics.

桃褐腐病是桃树上的一种重要病害,导致桃果经济损失严重.本文对不同产区桃褐腐病菌的生物学特性进行了研究.来源不同产区的桃褐腐菌株总体在PDA培养基、25℃、pH值6.0～7.0及每天光照12h条件下生长最好;菌丝生长最适碳源和氮源分别是淀粉和牛肉浸膏.褐腐菌株分生孢子平均在25℃、pH值7.0及每天光照12h条件下萌发率最高.不同产区褐腐菌株间存在生物学差异,表明病菌存在丰富的生理生化多样性.研究结果表明,不同的营养、温度、光照、pH值等环境条件对桃褐腐病菌菌丝生长和孢子萌发有显著影响.

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21世纪是知识经济时代，知识员工是现代社会的主流工作者，是企业管理的重要资源。很多研究已经证实自我效能感可以促进员工工作绩效的提升，但自我效能感的影响因素研究较少，文章着重探究影响自我效能感的因素。

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