This research aims to analyze morphometrics of (P. penicillatus) and (P. versicolor) in waters of Sabang. A total of 50 samples from each species of P. penicillatus and P. versicolor were used for morphometric analysis using Traditional Morphometric and Truss Morphometric approaches. Morphological analysis indicated that both lobster species are categorized into two distinct groups. Traditional morphometric character testing using ANOVA showed that species differences significantly influence the variation in morphological characters (P<0.05) in both lobsters. Further Duncan post hoc tests revealed that four characters (HL), (CHW), and (CHL)) differed significantly between the species. P. penicillatus shared three characters similar to P. versicolor, namely (HL), (CHW), and (CHL). This similarity is based on the shape of HL, CHW, and CHL in both lobsters. In the Truss Morphometric character testing with ANOVA, it was found that species differences affect the characters significantly (P<0.05). The Duncan post hoc test revealed that five characters ([A1, A3], [B1, B3], [C5, C6], D4, [D5, D6]) differed significantly between species. DFA resulted in 1 function, with an eigenvalue of 27.595, indicating that it explains 100% of the total variance. The morphological characters that significantly contribute to the differentiation between species are A1, B1, C5, A5, and D5.

Abstract. Abbas MA, Iqbal M, Firdus F, Sari W, Putra DF. 2024. Histological description of hepatopancreas in spiny lobster (Panulirus penicillatus) and bamboo lobster (Panulirus versicolor) in Sabang Waters, Aceh, Indonesia. Biodiversitas 25: 3739-3749. This study aimed to analyze the histology of the hepatopancreas in two lobster species, Panulirus penicillatus (Olivier, 1791) and Panulirus versicolor (Latreille, 1804), originating from Sabang waters. The primary objectives were to identify and compare the cellular composition and structural differences within the hepatopancreas of both species and to understand how these differences might reflect adaptations to their distinct local environments and dietary conditions. The histological analysis was conducted on the hepatopancreas of 10 specimens from each species using the paraffin section method and Hematoxylin-Eosin (H&E) staining. The results showed striking differences in the tubule cells between the two species. In P. penicillatus, B-cells were found to be more dominant, whereas in P. versicolor, F-cells were more prevalent. Additionally, the study observed significant variations in the size and arrangement of the Tubules, as well as the distribution of different cell types within the hepatopancreas. These differences likely reflect adaptations to their distinct local environments and dietary conditions in Sabang waters. The hepatopancreas, a vital organ for digestion, nutrient absorption, and detoxification, showed distinct histological features in the two lobster species. Understanding these variations provides valuable insights into their biological and physiological adaptations. The research highlights the importance of species-specific studies to comprehend the ecological and physiological adaptations of marine organisms. Furthermore, these findings have significant practical implications for the management and cultivation of these lobster species. By understanding the histological differences and their underlying causes, better strategies can be developed for the conservation and sustainable exploitation of these valuable marine resources. The study underscores the role of histological analysis in assessing the health and condition of lobster populations, which is crucial for effective management.

Background:The giant trevally,Caranx ignobilis, is a commercially important marine fish in Indonesia. This species was initially cultured in Aceh Province. Previous reports showed that charcoal has a positive effect on survival and feed utilization of the giant trevally. However, the effects of adding charcoal to the diet on gut and intestine biometrics has, to our knowledge, never been described.Methods:Four activated charcoal sources were tested in this study using a completely randomized experimental design; coconut shell charcoal, mangrove wood charcoal, rice husk charcoal, and kernel palm shell charcoal. All treatments were performed with four replications. Juvenile giant trevally (average body weight, 16.52 ± 3.12 g; and average total length, 10.26 ± 0.64 cm) were stocked into the experimental tank at a density of 15 fish per tank. The fish were fed an experimental diet twice daily at 7 AM and 5 PMad satiationfor 42 days.Results:Analysis of variance showed that adding charcoal to the diet had significant effects on the length and width of the foveola gastrica and villous intestine (P < 0.05). The greatest length and width of the foveola gastrica was recorded in fish fed an experimental diet of rice husk charcoal with average values of 311.811 ± 9.869 µm and 241.786 ± 10.394 µm, respectively. The greatest length of intestinal villous was found in fish fed the mangrove wood charcoal diet, with a value of 135.012 ± 5.147 µm, but this length was not significantly different to that in fish fed rice charcoal and kernel palm shell charcoal. However, the greatest width of intestinal villous was recorded in fish fed the control diet (without charcoal; P < 0.05).Conclusion:The optimal sizes of the foveola gastrica and villous intestine were found in fish fed an experimental diet with rice husk charcoal.

Data of feed conversion ratio (FCR) and survival rate (SR) of Caranx ignobilis

Background: Research on supplementing feed with rice husk activated charcoal was carried out to determine the effect of variations in the concentration of rice husk activated charcoal on the growth and histological features of the giant trevally Caranx ignobilis intestine. Methods: This study used an experimental method with a completely randomized design consisting of six treatments and four replications, including adding activated charcoal to feed at concentrations of 0%, 1%, 1.5%, 2%, 2.5%, and 3% for 42 days. The measured parameters included daily growth rate (DGR), specific growth rate (SGR), absolute growth rate (AGR), feed conversion ratio (FCR), feed efficiency (FE), survival rate (SR), length of foveola gastrica, width of foveola gastrica, length of intestinal villi, and width of intestinal villi. Data were analyzed statistically using one-way analysis of variance and Duncan’s test. Results: The results showed that supplementing fish feed with rice husk activated charcoal at different concentrations significantly affected the values of DGR, AGR, FCR, FE, SR, length of the foveola gastrica, length of the villous intestine, and width of the villous intestine, but did not significantly affect SGR or foveola gastrica width. Conclusion: The 2% rice husk activated charcoal treatment showed the best results for all parameters.