FeCrCo magnetic films are suitable candidates for use as magnetic code disk materials in high-accuracy magnetic encoders. The performance and material uniformity of FeCrCo films are crucial for high-accuracy servo control of the encoder. However, enhancing coercivity and maintaining material uniformity while ensuring sufficient remanence in FeCrCo films pose challenges. Herein, Pt (t nm)/FeCrCo (100 nm) films were prepared via magnetron sputtering and subsequent vacuum annealing. The remanence of the FeCrCo (100 nm) monolayer film is 520 emu/cm3, with a coercivity of only 380 Oe. However, after using Pt as the buffer layer, the remanence of Pt (1 nm)/FeCrCo (100 nm) and Pt (2 nm)/FeCrCo (100 nm) films is 320 emu/cm3 and 250 emu/cm3 respectively, while the coercivity increases to 520 Oe and 625 Oe respectively, representing improvements of 37 % and 65 %. This significant increase in coercivity is achieved with the remanence still meeting the performance requirements of the magnetic code disk. High-resolution transmission electron microscopy (HRTEM) analysis reveals that diffused Pt atoms can facilitate the formation of a small amount of non-magnetic gamma- FeCrCo phase, which has a pinning effect on magnetic domain walls, thus significantly increasing the coercivity of the FeCrCo film. Moreover, since the gamma- FeCrCo phase and the alpha-FeCrCo phase have similar crystal lattices, material uniformity can be maintained, which is beneficial for ensuring the uniformity of output signals of the code disk with small magnetic pole pitches.

Small cell lung cancer (SCLC) often exhibits Rb deficiency, TRβ and p130 deletion, and SKP2 amplification, suggesting TRβ inactivation and SKP2 activation. It is reported that SKP2 targeted therapy is effective in some cancers in vitro and in vivo, but it is not reported for the treatment of SCLC and retinoblastoma. SKP2 is the synthetic lethal gene in SCLC and retinoblastoma, so SKP2 can be used for targeted therapy in SCLC and retinoblastoma. RB1 knockout mice develop several kinds of tumors, but Rb1 and SKP2 double knockout mice are healthy, suggesting that SKP2 targeted therapy may have significant effects on Rb deficient cancers with less side effects, and if successful in SCLC and retinoblastoma in vitro and in animal model, such compounds may be promising for the clinical treatment of SCLC, retinoblastoma, and variety of Rb deficient cancers. Previously our studies showed that retinoblastomas exhibit retinal cone precursor properties and depend on cone-specific thyroid hormone receptor β2 (TRβ2) and SKP2 signaling. In this study, we sought to suppress SCLC and retinoblastoma cell growth by SKP2 inhibitors as a prelude to targeted therapy in vitro and in vivo. We knocked down TRβ2 and SKP2 or over-expressed p27 in SCLC and retinoblastoma cell lines to investigate SKP2 and p27 signaling alterations. The SCLC cell lines H209 as well as retinoblastoma cell lines Y79, WERI, and RB177 were treated with SKP2 inhibitor C1 at different concentrations, following which Western blotting, Immunostaining, and cell cycle kinetics studies were performed to study SKP2 and p27 expression ubiquitination, to determine impact on cell cycle regulation and growth inhibition. TRβ2 knockdown in Y79, RB177 and H209 caused SKP2 downregulation and degradation, p27 up-regulation, and S phase arrest, whereas, SKP2 knockdown or p27 over-expression caused p27 accumulation and G1-S phase arrest. In the cell lines Y79, WERI, RB177, and H209 treatment with C1 caused SKP2 ubiquitination and degradation, p27 de-ubiquitination and accumulation, and cell growth arrest. SKP2 inhibitor C1 significantly suppressed retinoblastoma as well as SCLC cell growth by SKP2 degradation and p27 accumulation. In vivo study also showed inhibition of tumor growth with C1 treatment. Potential limitations of the success of such a therapeutic approach and its translational application in human primary tumors, and alternative approaches to overcome such limitations are briefly discussed for the treatment of retinoblastoma, SCLC and other RB-related cancers.

<p>Figure S1. TRβ2 and TRβ1 inversely regulate retinoblastoma cell proliferation and cell cycle progression.Figure S2. Pituitary anterior lobe and intermediate lobe markers and Trβ isoforms expressed in pituitary tumors in Rb1+/- mice of different Trβ genetic backgrounds.Figure S3. EMI1 mediates TRβ2 regulation of SKP2.Figure S4. Rescue of Rb knockdown-induced cell cycle arrest and SKP2 down-regulation by ectopic Rb expression.Figure S5. Gene expression changes in response to RB1-KD in synchronized SKN-BE(2) neuroblastoma cells.Figure S6. TRβ1 and TRβ2 regulate proliferation and SKP2 and p27 expression in Rb-deficient MXF8000 myxofibrosarcoma cells.</p>

Supplementary Information. This file contains Extended Experimental Procedures including Table S1 (pLKO lentiviral shRNAs), Table S2 (Antibodies used in this study), and Table S3 (qPCR primers).

FeCoCr-based films have been considered as promising candidates for next-generation magnetic code disk ma-terials used in high-accuracy magnetic rotary encoders. However, realizing high coercivity with sufficient remanent magnetization in FeCoCr-based films with in-plane anisotropy to ensure suitable disturbance-rejection performance and detectability of magnetic pole pairs is a critical issue, which hinders their practical applications. In this study, we demonstrated an in-plane anisotropic [Ta(20 nm)/FeCoCrMoTi(100 nm)](2)/Ta(10 nm) multi-layer film (M20 sample) with a high coercivity of 945 Oe and a sufficient remanent magnetization of 2890 Oe, which is 92.1% and 63.1% higher than FeCoCrMoTi(200 nm)/Ta(10 nm) film (SL sample), respectively. Detailed microstructure characterization using atomic resolution scanning transmission electron microscopy (STEM) indicates that the high coercivity originates from the domain-wall pinning effect induced by three factors including grain refinement and heterogeneous phases generation and composition fluctuation of modulated phases. X-ray absorption fine structure (XAFS) and X-ray photoelectron spectroscopy (XPS) analyses reveal that the high remanent magnetization is ascribed to the higher relative content of Fe2Ta phases with strong ferromagnetism than that of Co2Ta phases with weak ferromagnetism.

FeCoCr thin films have been considered as promising candidates for magnetic code disk materials used in high-precision magnetic rotary encoders. However, realizing high coercivity with a sufficient remanence is a critical issue for magnetic recording stability from the point view of practical applications. We prepared Bi-free and Bi-doped FeCoCr(250 nm)/Ta(10 nm) films on Si substrates by magnetron sputtering. The in-plane coercivity of Bi-free and Bi-doped FeCoCr samples are 414 Oe and 930 Oe respectively, indicating that the coercivity can be greatly enhanced by Bi doping. X-ray photoelectron spectroscopy (XPS) reveals that the Bi atoms diffuse from the bulk to the film surface and then sublimate during high-temperature annealing process. Positron annihilation spectroscopy (PAS) indicates that the diffusion of Bi atoms increases the density of defects in the film. Scanning transmission electron microscopy (STEM) analysis shows that the diffusion behavior of Bi atoms not only refines the grain size of the FeCoCr film but also results in a uniform distribution of the Fe-Co-rich (α1 phase) phase with the Cr-rich (α2 phase) phase. Magnetic force microscopy (MFM) analysis observes that the introduction of Bi decreases the magnetic domain size and increases the magnetic domain density, which resulting in the enhancement of coercivity.

As an important indicator of sexual maturity of hens, age at first egg (AFE) is significantly associated with reproduction performance. In this study, 400 hens were divided into 6 groups based on AFE to analyze the difference of reproduction performance, reproduction hormone levels and the characterization of the hypothalamo-pituitary-gonadal axis in chickens. The results showed that the egg production of the hens in the late-maturing groups was significantly lower than that of the ones in other groups and the precocious hens had a lower egg production rate. The hens in late maturing group had a lower fertility rate, LH levels and shorter duration of peak of egg production (PEP), the precocious hens had lower PRL levels. In addition, the characterization of the hypothalamo-pituitary -gonadal axis showed that the individuals with normal AFE had higher GNRH, GNRHR, ESR1, KITLG, and CYP11A1 expression levels than late-maturing and precocious individuals, which indicated that the chickens with normal AFE advantages on reproduction regulation system.

城市网格化管理理念是城市管理的创新模式,在城市管理中成效显著.中压配电网具有网络复杂,覆盖面广,配电点多的特点,也是各类新概念、新技术的主战场,其空间布局规划不能照搬高压布局的模式,需要探索创新,呼应国土空间规划的时代要求.今通过对配电网的特征进行分析,借鉴网格化理念,结合精细化管理、精益化投资的要求,以实际项目实践,去探索规划的深度、细度、精度,供相关规划工作者参考.

介绍胺基化生产工艺的物质性质、工艺过程、应急处理等多方面,进行胺基化工艺的危险性分析.从安全技术和安全管理的不同角度,提出针对性的控制措施,确保胺基化生产实现本质安全.

RFamide-related peptides (RFRP) are synthesized by the hypothalamus and have a regulatory role in gonad development. The goal of this study was to investigate the association between SNP of the RFRP gene and the reproductive traits and hormone levels of Zhenning yellow chickens. The mRNA expression levels were detected based on different tissues, ages, and genotypes. Eleven mutation sites were detected in the RFRP gene, 4 of which were significantly related to reproductive traits and hormone levels. Association analysis revealed that A276G was associated with egg production at 300 d of age (EP300) and amount of prehierarchical follicles (P < 0.05). G1396A was associated with egg weight at 300 d of age and luteinizing hormone (LH) and prolactin levels (P < 0.05). G1694A showed significant associations with fertilization rate and LH levels (P < 0.05), and A2659G was associated with EP300 (P < 0.05). The results of expression analysis showed that the RFRP mRNA expression levels in the hypothalamus were higher than those in other tissues (P < 0.01). The expression in immature individuals was higher than that in mature ones (P < 0.01). There were also differences in mRNA expression levels between different genotypes (P < 0.05). In summary, the results of this study might provide potential markers and a theoretical basis for the improvement of chicken reproductive traits.

In this article, a general hybrid beam-forming model was used to analyze the difference between x kinds of active antenna array architectures. A two-dimension diagram and a figure of merit with two digitization factors wereproposed to assess the digitization level of these different architectures. Also, based on a cascade methodology, we conducted theoretical analysis on the sensitivity and dynami...

ABSTRACT 1. H-FABP (heart-type fatty acid-binding protein) is an important fatty acid-binding protein that participates in the metabolism and intracellular transportation of lipids. This study was designed to study the H-FABP gene expression and genetic association with meat quality traits in domestic pigeons. 2. The spatio-temporal expression patterns showed that H-FABP was widely expressed in all eleven tissues in birds from hatching to 4 weeks of age, and the expression level in the liver was the highest, and spleen and subcutaneous fat showed relatively high levels at all time points, and increased markedly as squabs became older. 3. Nine novel SNPs were found; three of them (g.42 C > T and g.123 C > T in exon 1 and g.3128 C > T in exon 2) were synonymous mutations, and six of them (g.3304 T > C, g.3305 G > A, g.3313A>G, g.3343 C > T, g.3372A>C, g.3410 T > C in exon 3) were located in the 3ʹ untranslated region (UTR). In addition, four haplotypes and six diplotypes were formed and showed weak linkage disequilibrium. Association analysis showed that in the SNP of g.3305 G > A, the AB genotype showed higher (P < 0.05) inosinic acid concentrations in breast muscle than the BB genotype. In the SNP of g.3313A>G, the AA and AB genotypes showed significantly higher (P < 0.01) inosinic acid concentrations than the BB genotype, AA showed the highest intramuscular fat content, and the relative mRNA expression level of the AA genotype was the highest (P < 0.05). 4. Association analysis of diplotypes showed no correlation with meat quality traits. Moreover, correlation analysis indicated that the H-FABP mRNA expression level was tightly related to intramuscular fat content (P < 0.01). 5. This study suggested that the H-FABP gene may be a candidate gene in marker-assisted selection of pigeons for high-quality meat.

以三栋具有代表性的幼儿园、小学、中学隔震建筑为背景,对已建成的厦门市学校隔震工程进行隔震技术应用的调研和技术分析.首先从政府职能部门的服务和管理角度,以及从建设相关单位管理和技术角度这两个方面指出隔震技术推广应用过程中存在问题并给出相应的建议.分析表明:政府职能部门需要完善与隔震技术应用相关的政策,大力推广隔震技术的应用;建设相关单位应加强隔震技术的学习,提升隔震工程设计、施工、监理监督等方面的管理和技术水平.

In this paper, a new procedure is proposed for simulation of the time-varying wideband radar scattering characteristics of targets above sea surface. A geometric model of the composite target-sea scene is developed by combination of target aerodynamics and time-varying sea surface generation. Multipath scattering model is applied to address the electromagnetic (EM) scattering interaction between t...

Mechanical equipment fault diagnosis technology is a new research field with the development of modern science and technology In recent years, the deep learning has been applied into the field of mechanical equipment fault diagnosis by more and more researchers. Deep learning has been widely applied in various in dustries and fields with its unique advantages in feature extraction and pattern recognition. The development history of deep learning is first briefly reviewed. Then, four fault diagnosis methods based on deep learning model are emphatically analyzed com bined with the characteristics and requirements of fault diagnosis technology for large rotating machinery equipment, and the problems and challenges they face are discussed. Finally, the research direction worth to be carried out in the future is prospected.

介绍了以对二氯苯和浓硝酸为原料、无水硫酸镁为催化剂制取2,5-二氯硝基苯的工艺过程;分析了催化剂用量和催化剂循环利用对产物选择性的影响,确定了适宜的催化剂用量和催化剂酸化条件.

在配电网规划中引入网格化理念,是配电网的规划、建设、运行、管理与城乡空间规划的无缝衔接,有利于提高配电网规划质量和运行管理水平,有利于配电网的精准投资和精益管理.结合配电网网格化规划的相关指导原则,本文分析配电网与城乡空间规划的层级结构特征,提出了配电网网格划分的"三要素"法,即结合自然地理要素、社会经济要素和电网要素,快速识别网格界线,合理划分网格,达到配电网网格与城乡空间规划的有效结合.通过案例实践,具有一定的合理性,可供相关研究参考.

Whether pyrolysis gases combustion has effect on surface ablation of charring material is a key issue in the optimization design of thermal protection system for hypersonic vehicles. The coupled method considering combustion of pyrolysis gases in shock layer is presented, realized in MATLAB and validated by comparison with the arcjet experiments. Relationship between surface ablation and mole fraction of oxygen, mass injection rate of pyrolysis gases and surface temperature of charring material under different Mach numbers are predicted by this coupled method. Numerical results indicate that the protective effect of pyrolysis gases combustion on surface ablation is more and more obvious with increase of Mach number. This study is helpful to the optimization of thermal protection system for hypersonic vehicles.

We report a large enhancement of spin–orbit torque (SOT) in perpendicular Ta/CoFeB/MgO multilayers with interfacial H+ and O2– ion manipulations. By controlling both H+ and O2– ions at the CoFeB/MgO interface, the switching current density (Jc) is almost half of that for the single O2– ion manipulated sample. Through harmonic measurements, we have found that both dampinglike effective field HD and fieldlike effective field HF are increased for the H+ and O2– ion manipulated samples. Interfacial structural results indicate that the H+ and O2– ion manipulations modulate the interfacial chemistry at the CoFeB/MgO interface, which suppresses the spin reflection and improves the spin absorption in the CoFeB layer. Our results can effectively improve the SOT and provide an effective way to modulate SOT.