Objective: To study the growth, multiplication and expression of porcine hepatocytes in the three-dimensional culture system (hollow fiber bioreactor, HFB) so as to find out the relationship of the vitality and culture time of hepatocytes and find out how long the bioartificial liver (BAL) in this culture system can offer an effective hepatic function support. Methods: Hepatocytes were isolated from hybrid pigs weighing 5-8 kg by means of colagenase. After isolating and counting, we put the suspending hepatocytes (5×107-10×107·mL-1) into the inside space of the HFB. In the beginning, we circuited the culture medium through outside space of the HFB. When the hepatocytes were glued on the side of the tube that had been stuck with little gel matrix, we changed the circuiting culture medium through inside space of the HFB. At regular intervals we observed and checked the vitality, conformation, growth and expression of the cultured hepatocytes. Results: Porcine hepatocytes not only grew and reprocessed well in HFB but also expressed well in function. They could remain vigorous for about 35 days and take on an usual survival pattern to some extent. Conclusion: The porcine hepatocytes cultured in this system can meet the requirements of the BAL support treatment from 5 to 25 days. During this period the porcine hepatocyte-hollow fiber BAL can be applied experiment and clinical research.

目的探讨P53、P21(WAF1/CIP1)基因与甲状腺癌的发生、发展及预后的关系以及它们的相互关系.方法应用S-P免疫组化技术,检测60例甲状腺癌、10例正常甲状腺组织、10例结节性甲状腺肿、10例甲状腺腺瘤组织中P53、P21(WAF1/CIP1)的表达.结果 P53、P21(WAF1/CIP1)阳性反应仅见于甲状腺癌,其阳性率分别为53.3%、41.7%;P53阳性率在未分化癌、有淋巴结转移或Ⅲ、Ⅳ期病例中增高(P<0.05);但P21(WAF1/CIP1)阳性率在未分化癌、有淋巴结转移或Ⅲ、Ⅳ期病例中降低(P<0.05);P53表达与P21(WAF1/CIP1)表达关系密切(P<0.05).结论 P53突变、P21(WAF1/CIP1)缺乏均与甲状腺癌发生有关;P21(WAF1/CIP1)在甲状腺癌中表达主要依赖于P53;P53、P21(WAF1/CIP1)在甲状腺癌中表达的变化可用于评估其生物学行为和判断预后.