OBJECTIVE:To observe the effects of Huaiqihuang granules on the immune function in children with severe Mycoplasma pneumoniae pneumonia.METHODS:Pediatric inpatients with severe Mycoplasma pneumoniae pneumonia were randomly divided into Huaiqihuang granule treatment group (n=51) and conventional treatment group (n=47). The Huaiqihuang granule treatment group was orally administered Huaiqihuang granules in addition to the conventional treatment, while the conventional treatment group received conventional treatment only. Levels of serum IgA, IgG, and IgM, percentages of CD4+ and CD8+ T lymphocyte subsets, and CD4+/CD8+ ratio were examined in the two groups. The incidence rate of respiratory tract re-infection within three months following treatment was compared between the two groups.RESULTS:The levels of serum IgA, IgG, and IgM, the percentage of CD4+ T lymphocytes, and the CD4+/CD8+ ratio were significantly higher in the Huaiqihuang granule treatment group than in the conventional treatment group three months after treatment (P<0.05). In contrast, the percentage of CD8+ T lymphocytes was significantly lower in the Huaiqihuang granule treatment group than in the conventional treatment group (P<0.05). In addition, the incidence rate of respiratory tract re-infection within three months following treatment was significantly lower in the Huaiqihuang granule treatment group than in the conventional treatment group (P<0.05).CONCLUSIONS:Huaiqihuang granules can regulate immune functions and reduce the incidence of short-term respiratory tract re-infection in children with severe Mycoplasma pneumoniae pneumonia.

Throat swabs from children with suspected Mycoplasma pneumoniae (M. pneumoniae) infection were cultured for the presence of M. pneumoniae and its species specificity using the 16S rRNA gene. Seventy-six M. pneumoniae strains isolated from 580 swabs showed that 70 were erythromycin resistant with minimum inhibitory concentrations (MIC) around 32-512mg/L. Fifty M. pneumoniae strains (46 resistant, 4 sensitive) were tested for sensitivity to tetracycline, ciprofloxacin, and gentamicin. Tetracycline and ciprofloxacin had some effect, and gentamicin had an effect on the majority of M. pneumoniae strains. Domains II and V of the 23S rRNA gene and the ribosomal protein L4 and L22 genes, both of which are considered to be associated with macrolide resistance, were sequenced and the sequences were compared with the corresponding sequences in M129 registered with NCBI and the FH strain. The 70 resistant strains all showed a 2063 or 2064 site mutation in domain V of the 23S rRNA but no mutations in domain II. Site mutations of L4 or L22 can be observed in either resistant or sensitive strains, although it is not known whether this is associated with drug resistance.

肺炎支原体(Mp)是儿童和青少年呼吸道感染的常见病原体,大环内酯类抗生素是治疗Mp感染的首选药物.以红霉素为代表的大环内酯类抗生素作用机制是通过抑制蛋白质的合成而发挥抗菌作用,即大环内酯类抗生素结合于核糖体50S亚基的转肽酶中心与肽输出通道狭窄之间的部分,通过机械性的阻塞通道而抑制肽的延伸,从而阻碍蛋白质的合成[1-3].其在大亚基上(50S亚基)的结合位点是23SrRNA结构域V中心环的保守碱基A2058-A2062和U2609[4-5].

2003年6月-2006年6月,我们通过药物敏感试验测定肺炎支原体(MP)分离株庆大霉素、四环素、环丙沙星的最小抑菌浓度(MIC),以了解庆大霉素、四环素、环丙沙星对MP的抗菌活性,寻求对MP尤其是耐药MP有效的药物.

Fifty clinical Mycoplasma pneumoniae strains were isolated from 370 children with respiratory tract infections. Four strains were susceptible to macrolides, while the other 46 (92%) were macrolide resistant. The molecular mechanism of resistance was shown to be associated with point mutations in 23S rRNA at positions 2063 and 2064.

Objective To explore the antibacterial activity of chloramphenicol and rifampin to mycoplasma pneumoniae(MP).Methods Three hundred and seventy throat swab specimens were cultured to isolate MP.Clinical isolated strains were identified by nested polymerase chain reaction(PCR) of MP species specific 16SrRNA gene.Minimal inhibitory concentration(MIC) of erythromycin was determined by antibiotic susceptibility test and 23SrRNA gene was amplified by nested PCR to identify macrolide resistant strains.MIC of rifampin and chloramphenicol were determined by antibiotic susceptibility test.Results Fifty clinical strains were isolated from 370 specimens.Of 50 strains,4 strains were susceptible to erythromycin,while 46 strains were erythromycin resistant.Forty-six resistant strains had a point mutation respectively in 23SrRNA gene,4 susceptible strains had not point mutation.All isolated strains were susceptible to chloramphenicol and resistant to rifampin.Conclusion Rifampin is not effective to MP,and Chloramphenicol is effective to erythromycin susceptible and resistant MP strain.

Objective To understand the epidemic regulation of mycoplasmal pneumonia in Beijing and the carriageable time of mycoplasma pneumonia (MP) after affecting mycoplasmal pneumonia.Methods The data of 1929 inpatients with mycoplasmal pneumonia in the department of pediatrics were statisted. And the incidences in different quarters, ages and years were analyzed. Twenty patients with mycoplasmal pneumonia were treated.Results There were no difference of discrepancy in the first quarter, the second and the third,all P0.05. The first quarter, the second, the third respectively compared with the fourth, all P0.05. Six to nine years old compared with 9-14 years old, P0.05. One month to 3 years old compared with 4-6 years old, 7-9 years old, 10-14 years old, all P0.05.Four to six years old compared with 7-9 years old, 10-14 years old, P0.05.The incidence declined gradually in 1992-1994. The incidence was the highest in 1995. The shortest carriageabe time of MP was a half month.The average carriageabe time of MP was one and half months. The longest carriageabe time of MP was four and half months. Conclusions The incidence of MP was closely related to season,age and year.The carriageabe time of MP after the onset differed with different patients,and the correlation factors should be further studied.