This research was carried out to assess the effect of E-cinnamaldehyde (EC) against Sclerotinia sclerotiorum, causal agent of potato white mold, under invivo and invitro conditions. Based on the results, EC was able to completely inhibit (P < 0.05) mycelial growth of four isolates in both volatile and contact phases after five days of inoculation at the concentrations 500 μl and 5 μl/ml, respectively. Moreover EC at concentrations 30 and 50 μl EC/ml sterile distilled water significantly reduced white mold on six potato cultivars including Pashandi, Istambouli, Agria, Marfauna, Alpha and Spartaan in greenhouse trials. In next step, induction of glutathione S-transferase (GST)-like genes identified from the pathogen (isolate 3) genome was assessed. After 2 h of exposure to both EC and cinnamom extract, four genes with locus numbers SS1G_07319.1, SS1G_07195.1, SS1G_00703.1 and SS1G_01918.1 were up-regulated. Furthermore, EC and cinnamon extract appeared to have no effect on gene expression in SS1G_10295.1. In this study, enzyme activity of GST was determined with 1-chloro-2, 4-dinitrobenzene as substrate. While enzymatic activity was 42.3 and 45.6 units mg−1 protein in sterilized distilled water and ethyl acetate, respectively, it was recorded as 78.9 and 86.4 units mg−1 protein for cinnamon extract and EC, respectively.

This study was conducted to determine the inhibitory effect of E-cinnamaldehyde (EC) against causal agent of storage carrot rot, Sclerotinia sclerotiorum, under in vivo and in vitro conditions. Based on the results, EC was able to completely inhibit mycelial growth of three isolates (P>0.05) in both volatile and contact phases after 6days at the concentrations 200μl and 1μl/ml, respectively. In addition, EC at concentrations 1 and 10μl/ml completely inhibited carpogenic germination of three isolates. The results of in vivo trials showed that EC at the concentration of 10μl/ml was able to control the disease caused by isolates 1 and 3. However the disease caused by isolate 2 was inhibited with the concentration of 20μl/ml. In enzyme analyses, the activity of polyphenoloxidase and peroxidase did not change in the inoculated carrots after application of EC. Furthermore, the level of phenylalanine ammonia lyase decreased. These results indicated that EC does not have any potential to be considered as resistance inducers against sclerotinia carrot rot.