The authors report no conflict of interest. All supporting data of this article are included in the submitted manuscript.

Journal of the European Academy of Dermatology and VenereologyVolume 36, Issue 9 p. e708-e710 Letter to the Editor Genotype-guided targeted treatment of KRAS-mutated arteriovenous malformations R. Cai, R. Cai Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-first authors.Search for more papers by this authorZ. Wang, Z. Wang Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-first authors.Search for more papers by this authorY. Sun, Y. Sun orcid.org/0000-0002-6154-5341 Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-first authors.Search for more papers by this authorX. Yang, X. Yang Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorM. Wen, M. Wen Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorL. Zheng, L. Zheng Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorD. Wang, D. Wang Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorL. Yu, Corresponding Author L. Yu yulan20@zju.edu.cn Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, ChinaThese authors contributed equally to this work and should be considered co-corresponding authors.Correspondence: L. Su, X. Fan and L. Yu. E-mails: sulixin1975@126.com(LS); fanxindong@aliyun.com(XF) and yulan20@zju.edu.cn(LY)Search for more papers by this authorX. Fan, Corresponding Author X. Fan fanxindong@aliyun.com Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-corresponding authors.Correspondence: L. Su, X. Fan and L. Yu. E-mails: sulixin1975@126.com(LS); fanxindong@aliyun.com(XF) and yulan20@zju.edu.cn(LY)Search for more papers by this authorL. Su, Corresponding Author L. Su sulixin1975@126.com Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-corresponding authors.Correspondence: L. Su, X. Fan and L. Yu. E-mails: sulixin1975@126.com(LS); fanxindong@aliyun.com(XF) and yulan20@zju.edu.cn(LY)Search for more papers by this author R. Cai, R. Cai Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-first authors.Search for more papers by this authorZ. Wang, Z. Wang Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-first authors.Search for more papers by this authorY. Sun, Y. Sun orcid.org/0000-0002-6154-5341 Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-first authors.Search for more papers by this authorX. Yang, X. Yang Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorM. Wen, M. Wen Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorL. Zheng, L. Zheng Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorD. Wang, D. Wang Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaSearch for more papers by this authorL. Yu, Corresponding Author L. Yu yulan20@zju.edu.cn Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, ChinaThese authors contributed equally to this work and should be considered co-corresponding authors.Correspondence: L. Su, X. Fan and L. Yu. E-mails: sulixin1975@126.com(LS); fanxindong@aliyun.com(XF) and yulan20@zju.edu.cn(LY)Search for more papers by this authorX. Fan, Corresponding Author X. Fan fanxindong@aliyun.com Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-corresponding authors.Correspondence: L. Su, X. Fan and L. Yu. E-mails: sulixin1975@126.com(LS); fanxindong@aliyun.com(XF) and yulan20@zju.edu.cn(LY)Search for more papers by this authorL. Su, Corresponding Author L. Su sulixin1975@126.com Department of Interventional Therapy, Multidisciplinary Team of Vascular Anomalies, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai, ChinaThese authors contributed equally to this work and should be considered co-corresponding authors.Correspondence: L. Su, X. Fan and L. Yu. E-mails: sulixin1975@126.com(LS); fanxindong@aliyun.com(XF) and yulan20@zju.edu.cn(LY)Search for more papers by this author First published: 21 April 2022 https://doi.org/10.1111/jdv.18165 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume36, Issue9September 2022Pages e708-e710 RelatedInformation

Ischaemic stroke is one of the leading causes of global mortality as well as global burden of long-term disability1,2. Our aim was to explore the novel role of lncRNA SNHG12 and its potential target genes in primary neurones subjected to an oxygen-glucose deprivation/reoxygenation (OGD/R) injury. Primary neurones were prepared and then subjected to OGD/R injury in accordance with the instructions provided by the manufacturer. Quantitative polymerase chain reaction (PCR) was carried out to measure the expression level of SNHG12 under various conditions. In situ hybridisation (ISH) was performed to determine the special distribution of SNHG12 in primary neurone. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) assay, and western blot were used to evaluate neurotoxic effects in primary neurones. Additionally, the expression level of Akt was also measured using western blot. After OGD/R treatment, neurotoxic effect were observed in the primary neurone, in which both the rates of LDH release and cell survival were significantly increased, whereas cell death decreased. Notably, there was an apparent increase in ischaemic damage after OGD/R treatment, especially for the OGD12h/R24h treatment. For instance, the expression level of SNHG12 reached its peak after OGD12h/R24h. Moreover, SNHG12 knockdown increased the susceptibility to the neurotoxic effects of OGD/R treatment, in particular for the significant increases in LDH release (Fig. 2) and cell survival. Accordingly, the expression of Bcl-2 was down-regulated, whereas the expression of Bax was apparently up-regulated, thereby allowing the decreasing trend in Bcl-2/Bax ratio. Nevertheless, the expression of Akt was unaffected by OGD/R treatment. Meanwhile, expression level of p-Akt was decreased, and also p-Akt/Akt ratio. SNHG12 attenuates OGD/R-mediated neurotoxicity through the Akt signalling pathway in primary neurones. Natural Science Foundation of Shanghai (18ZR1422900).