The transcriptomes of skin were sequenced in two types of monochromatic red common carp（Cyprinus carpio） with body length of（33.42 ±0.24） cm and yellow common carp with body length of（38.75±0.43） cm using Illumina Hi-seq sequencing technology, and differentially expressed genes were identified and selected for analysis in order to investigate the genetic resources of whole red and yellow common carp. The results showed that 129 222 850 clean reads were obtained in whole red and 130 869 572 in whole yellow common carp. Comparison with known genes 58 782 genes were annotated in the Nr, 61 490 in Nt, 11 468 in COG, 31 206 in GO, 31 581 in KEGG, 61 385 in Pfam and 51 805 in Uniprot databases, with 8 378 genes annotated in all seven databases. There were 4 822 differentially expressed genes in skins of whole red and yellow common carp, with 1 929 significantly up-regulated and 2 893 significantly down-regulated. Further clustering analysis of differentially expressed genes revealed similar patterns of high and low expression of differentially expressed genes in both skin samples. The reliability of transcriptome data results was confirmed by qRT-PCR validation of 9 differentially expressed genes, in which some genes found here were involved in body color regulation, such as mitfa, ednrA, wnt7, agouti and mif. KEGG analysis revealed that differential genes were significantly enriched in 43 pathways, including mannose and fructose metabolism, glycolysis/gluconeogenesis, carbon metabolism, pentose phosphate pathway and tyrosine metabolism. The analysis of tyrosine metabolism and melanin synthesis pathways showed that mif, mif4g, agouti, ednrA and ednrB were significantly upregulated and tyr, tyrp1, mitfa and wnt7 were significantly downregulated in genes related to red and yellow pigment synthesis. Analysis of differential genetic variation indicated a total of 388 673 single nucleotide polymorphism（SNP） markers in both whole red and whole yellow common carp. The findings indicated that the differentially expressed genes in the skin tissues of two monochromatic common carp were primarily enriched in energy metabolism, growth, and immunity pathways. The transcriptome data obtained can be used to develop molecular markers as well as cloning, editing, and functional analysis of functional genes in koi.

为了分离纯化白背天葵多酚,并探讨其体外抗氧化活性,本研究比较了8种大孔树脂对白背天葵多酚的静态吸附和解吸能力,优选出D101为最适宜纯化白背天葵多酚的树脂,并对其分离纯化的工艺进行了优化.采用DPPH和ABTS法评价其抗氧化活性.结果表明,D101树脂最佳纯化工艺为上样液浓度1 mg/mL、pH 3.1、上样液流速2 mL/min,解吸的乙醇体积分数70％、体积60 mL、流速2 mL/min;白背天葵多酚的含量由14.73％提高到45.21％;其清除DPPH及ABTS自由基的能力为Vc>纯化物>粗提物.研究结果为进一步开发利用白背天葵多酚提供了数据支持.