Introduction: Our study aimed to find out whether the leaves of Acanthopanax gracilistylus with the ethanol extract have the antidiabetic effects in streptozotocin (STZ) induced type 1 diabetic rats. Materials and methods: Type 1 diabetic rats were induced by intraperitoneal STZ injection with 75 mg/kg. The animals were divided randomly with six rats in each group. The study lasted 28 days with daily administration, and at the end of the experiment, the rats were euthanized, the plasma/serum was separated for the biochemical parameters. The pancreatic tissue was used for histopathological and immunohistochemical measurement of NF-kappa B. Results: Treatments of Acanthopanax gracilistylus (100 mg/kg) group led to a significant reduction in diabetic rats in blood glucose was assessed at 2 h by the oral glucose tolerance test (OGTT). The treatment also resulted in insulin level (from 0.33 ng/ml reduced to 0.59 ng/ml), lipid profile (triglyceride lever was reduced from 206.18 to 139.16 mg/dl, and total cholesterol level was reduced from 129.08 to 100.04 mg/dl. By inhibiting NF-kappa B, the release of pro-inflammatory cytokines in diabetic rats induced by STZ was inhibited. In pancreatic tissues, Simultaneous observation of histopathological damage. Conclusion: These findings support the beneficial effect of the leaves of Acanthopanax gracilistylus treatment in diabetic rats, and we attribute it to its anti-diabetic effects.

目的 探索中国传统中药细柱五加叶水提取物降血糖和抗氧化作用.方法 采用MTT法测定细柱五加叶水提取物细胞毒活性;运用体外酶学试验,以阿卡波糖和熊果酸作为阳性对照,测定其α-葡萄糖苷酶、α-淀粉酶、基因重组人蛋白酪氨酸磷酸酶PTP1b的抑制活性评估其降血糖活性;以抗坏血酸为阳性对照,运用1,1-二苯基-2-三硝基苯肼(DP-PH)自由基,2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除法来研究其体外抗氧化活性.结果 细柱五加叶水提取物无细胞毒性,其体外酶抑制活性α-葡萄糖苷酶[IC50(138.50±1.21)μg·mL-1],α-淀粉酶[IC50(914.90±0.35)μg·mL-1]和 PTP1B[IC50(106.60±0.33)μg·mL-1].DPPH 和 ABTS 自由基清除活性的1C50值分别为(72.96±0.72)μg·mL-1和(74.86±0.33)μg·mL-1.结论 细柱五加叶水提物具有降糖和抗氧化作用.

目的:研究三叶五加茎的甲醇提取物及其各萃取物对α-葡萄糖苷酶的抑制活性,活性导向下对三叶五加茎的化学成分进行研究.方法:运用96微孔板测定三叶五加茎的甲醇提取物及其各萃取物的α-葡萄糖苷酶体外抑制活性.采用硅胶柱色谱、凝胶柱色谱、反相柱色谱及制备液相色谱等方法进行分离纯化,利用波谱分析结合理化性质鉴定化合物结构.结果:从三叶五加茎的甲醇提取物中分离得到17个化合物,分别鉴定为:蒲公英萜醇(1)、贝壳杉烯酸(2)、豆甾醇(3)、蒲公英萜醇乙酸酯(4)、β-谷甾醇(5)、3α,11 α-dihydroxy-23-oxo-lup-20(29)-en-28-oic aicd(6)、槲皮素-3-Oβ-D-吡喃半乳糖苷(7)、pubinernoid A(8)、紫丁香苷(9)、胡萝 卜苷(10)、豆甾醇葡萄糖苷(11)、正丁基-β-D-吡喃果糖苷(12)、邻苯二甲酸(13)、对羟甲基苯甲醛(14)、acankoreoside A(15)、acantrifoside A(16)、芦丁(17).结论:其中,化合物8、12、14为首次从该植物中分离得到,化合物8、12、14为首次从五加科植物中分离得到.

Objective: To find out whether the acankoreagenin, from the leaves of Acanthopanax gracilistylus, showed the hypoglycemic effects in streptozotocin (STZ)-induced diabetic rats. Methods: The diabetic rats were induced by a single intraperitoneal injection with STZ (70 mg/kg). At the end of the experiment the rats were euthanized, and the plasma/serum was separated for the determination of blood glucose, insulin, biochemical parameters. The pancreatic tissue was used for histopathological and immunohistochemical measurement of NF-kappa B. Results: Treatments of acankoreagenin (2 mg/kg) group led to a significant reduction in diabetic rats in blood glucose assessed via oral glucose tolerance test (OGTT) at 2 h. The treatment also resulted in HbA1c level (from 12.04% reduced to 7.01%), lipid profile (triglyceride lever was reduced from 145.83 to 74 mg/dl and total Cholesterol level was reduced from 101.33 to 78.66 mg/dl. Through inhibiting NF-.B, the proinflammatory cytokines release was suppressed in STZ-induced diabetic rats. In pancreatic tissues, histopathological injury was also observed. Conclusion: These findings are in support of the beneficial effect of acankoreagenin treatment in diabetic rats, and we attribute it to its hypoglycemic effects.