以龙牧801苜蓿叶片诱导产生的愈伤组织为材料，将其接种在诱导和胚性发生培养基上，采用形态学、涂片法和石蜡切片法对愈伤组织的形态和内部细胞结构进行观察。此外，研究了NAA和KT对愈伤组织芽分化的影响。结果表明：胚性愈伤组织和非胚性愈伤组织在形态和细胞学特性方面均存在明显差异，苜蓿愈伤组织芽分化最佳激素配比为0．5 mg／L NAA＋1．5 mg／L KT，研究结果将为苜蓿组培快繁奠定理论基础。
Taking alfalfa leaves on space flight as materials,the callus was induced.The half lethal concentration and lethal concentration of alfalfa callus under different concentrations of saline and alkali stress were studied,and 5 physiological and biochemical indexes under different saline-alkali stress were studied.The results showed that the half lethal and lethal concentration of alfalfa callus under NaCl stress were 1.2% and 1.8 mmol/L.The half lethal and lethal concentration of alfalfa callus under NaHCO3and Na2CO3stress were 0.4% and 1.0 mmol/L.The Alfalfa callus on space flight improved POD activity,soluble sugar and proline content,decreased the MDA content and relative conductivity,and the difference was significant.Space flight could improve saline alkali resistance of alfalfa callus,and alkali resistance was higher than that of salt resistance.
In order to improve the alfalfa's resistance to drought,the drought stress was simulated on leaf callus of alfalfa that was carried by satellite,and the response to PEG stress and 6 physiological and biochemical indexes were measured. The results showed that the rate of fatality increased gradually on alfalfa callus,with half lethal concentration and lethal concentration of PEG being 25% and 30%,as PEG concentration increased. In comparison with CK,satellite carrying significantly improved the contents of soluble sugar,soluble protein,and increased the POD and SOD activity,reduced the relative conductivity and malondialdehyde content. Satellite carrying increased drought resistance of the alfalfa callus,which could be useful for obtaining alfalfa mutants with high drought resistance.
以卫星搭载筛选出的2株突变株系为材料,研究空间环境对苜蓿(Medicago sativa)蛋白表达机制的影响,利用IEF/SDS-PAGE凝胶电泳技术,对其蛋白质特异性进行了比较分析.结果表明,突变株系与对照相比,蛋白质图谱有较大的差异,突变株系1L-4较对照共有6个蛋白质斑点下调,17个蛋白质斑点上调,突变株系6L-5较对照共有1 7个斑点下调,18个斑点上调,表明空间环境促使了苜蓿基因结构的改变,影响了蛋白质的表达.
