Objective:To preliminarily observe the clinical efficacy of microwave hyperthermia combined with intensity-modulated radiotherapy (IMRT) and chemotherapy for patients with locally advanced gastric cancer.Methods:Forty patients who could not been operated or refused operation were enrolled in this clinical trial, who were confirmed as locally advanced proximal or distal gastric cancer by gastroscopy pathology and imaging. Radiotherapy was delivered by IMRT technology for 5 times per week with a total dose of 46 to 56 Gy (median dose of 50 Gy) in 25 to 28 fractions. Synchronous hyperthermia was given at 42 to 44℃ twice a week, 45 min/time. S-1 or capecitabine-based synchronous chemotherapy was performed, d1-14/3 weeks. The symptom remission rate, adverse reactions, objective remission rate (complete and partial remission) and survival were observed.Results:A total of 40 patients, aged between 56 and 83 years (median age of 71 years), were enrolled in this study. The male-to-female ratio was 7: 1. Among them, 38 cases (95%) showed symptom remission. The most common adverse reactions were grade 1-2 gastrointestinal reactions and leukopenia. The objective remission rate was 87.5%, the 2-year progression-free survival and overall survival rates were 68.6% and 70.5%, respectively.Conclusion:Preliminary findings demonstrate that microwave hyperthermia combined with chemoradiotherapy achieve satisfactory outcomes and yield tolerable toxicity in patients with locally advanced gastric cancer.

目的:评价分析105例晚期肿瘤住院患者出现营养风险情况的相关因素.方法:选择2016年5月至2017年5月于我科就诊的105例晚期肿瘤住院患者作为此次的研究对象,运用营养风险筛查量表对所有患者出现营养风险情况进行评估.研究105例患者的病历,统计分析患者的住院时间、肿瘤位置以及典型症状,并分析患者住院时间与营养风险发生情况的相关性.统计分析两组患者的营养指标,并将统计分析的两组数据进行对比.结果:根据本文研究结果显示,105例患者中具有48.57%(51/105)的患者出现营养风险情况,其中56例消化系统癌患者出现营养风险情况(55.36%)概率最高;51例出现营养风险情况患者平均住院时间为(48.5±2.3)天.结论:晚期肿瘤住院患者中,消化系统癌患者更易出现营养风险情况,并且出现营养风险情况的患者往往相对于未出现营养风险情况的患者住院时间越长.

Objective It is to investigate the curative effect of“package” minimally invasive surgery in primary liver canc-er, and observe its influence on the serum T lymphocyte subsets ( CD3+, CD4 +, CD8 +, CD4 +/CD8 +ratio) and NK cells (CD56 +), matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF).Methods 85 patients with primary liver cancer were selected and divided into 3 groups according to the wishes of the patients:35 cases in group A were treated with transcatheter arterial chemoembolization ( TACE) , 30 cases in group B were treated with TACE +argon helium knife, 20 cases in group C were treated with TACE +argon helium knife frozen+125 I radioactive seed implantation; the changes of serum T lymphocyte subsets, CD56 +, MMP-9 and VEGF levels were observed before and after treatment, and the postoperative tumor shrinkage rate and recurrence rate and survival rate follow-up 16, 12 months were recorded in 3 groups. Results After 2 weeks of treatment, the serum levels of CD3 +, CD4 +, CD4 +/CD8 +and NK were significantly increased, and the levels of CD8 +, MMP-9 and VEGF were significantly reduced in the 3 groups (all P<0.05).After 5 weeks and 7 weeks treatment, the indexes of group B and C were significantly better than those after 2 weeks and those of group A in the same time (all P<0.05);at 7 weeks after operation, the improvement of the indexes of group C was significantly better than those at 5 weeks and those of group B in the same time (all P<0.05);at 12 weeks after operation, the tumor shrinkage rate of group B and C were significantly higher than that of group A ( P<0.05) , and group C was significantly higher than that of group B (P<0.05).The recurrence rate in group B and C was significantly lower, and the survival rates were significantly higher than that in the group A at 16 months follow-up (all P<0.05);and the recurrence rate of group C was significantly lower, survival rate was significantly higher than that of group B (all P<0.05).Conclusion The curative effect of“package”minimally invasive surgery in primary liver cancer is better, it can obviously improve the immune function of the body, inhibit tumor angiogenesis , reduce the recurrence rate and mortality, it is worthy of clinical popularization and application.

Objective To investigate the load cell lung cancer antigen autologous dendritic ( DCs )-induced T cell proliferation and cytotoxicity in vitro. Methods Lung cancer tissue, normal lung tissue and peripheral blood of fresh speci-mens were taken from 7 patients with squamous cell carcinoma who were treated with operation, normal lung tissue and lung cancer were prepared for single cell suspension. From the peripheral blood, mononuclear cells isolated and induced DCs;ny-lon wool columns were used to isolate the T cells. Using frozen melt method to prepare the autologous lung cancer cell lysates liquid, and stimulate the DCs, preparation of loaded with autologous lung cancer antigen DCs. Using MTS method to detect DCs complete antigen load of lung cancer on T cell proliferation;flow cytometry determination of DCs phenotype and the effect of load DCs complete antigen of lung cancer on T cell cytotoxicity. Results Peripheral blood mononuclear cells through GM-CSF, IL-4 and TNF-α’ s induce, a large number of dendritic protrusions, surface molecules CD14 expression was significantly lower ( t =20. 157, P <0. 01), CD1a, CD11c, CD80, CD83 and HLA-DR expression was significantly increased ( t =8. 927, t =3. 042, t =14. 311, t =18. 537, t =5. 612, P <0. 05, P <0. 01), mature DCs which was obtain from whole load of lung cancer antigen can stimulate autologous T cell proliferation, different patients revealed different stimulation index:6. 98-39. 15 (21. 56 ± 4. 38), and the higher the DC/T ratio, the higher the stimulation index, the stronger of T cells ability to respond to the proliferation ( t =31. 203, t =19. 550, P <0. 01);DCs of load lung cancer cell whole antigen can induce cytotoxicity of autologous T cells,and T cells to autologous tumor cell killing rate was(56. 52 ± 2. 75)%;normal cell average killing rate was (5. 31 ± 1. 37)%, significantly lower than for the autologous tumor cell killing rate ( t =16. 429, P <0. 01). The killing rate of K562 cell was (9. 71 ± 2. 46)%, which is significantly lower than the killing rate of autologous tumor cells (t = 25. 010, P <0. 01). Conclusion Load lung cancer complete antigen’s DCs can induce lung cancer pa-tients’ autologous T cell proliferation and cytotoxicity, and can effectively kill tumor cells in vitro, and normal cells were not killed.