Acyl-CoA Synthetase long-chain family member 4 (ACSL4) is a member of acyl-CoA synthetase protein long-chain family, which is associated with amino acid synthesis, lipid synthesis and lipid peroxidation dependent iron death. However, the role of ACSL4 in generalized carcinoma remains unclear. We aim to analyze the expression and prognostic value of ACSL4 in pan-cancer, and further explore the correlation between ACSL4 and immune infiltration. Through ONCOMINE, TIMER (Tumor Immune Estimation Resource), GEPIA (Gene expression Profiling Interactive), UALCAN and HPA, ACSL4 expression patterns of in pan-cancer were analyzed. The prognostic value of ACSL4 was analyzed using PrognoScan and Kaplan-Meier Plotter databases. Furthermore, gene variation and epigenetic modification of ACSL4 were analyzed by cBioPortal and GSCA databases. Meanwhile, GEPIA and TIMER databases applied to evaluate the relationship between ACSL4 expression and immune infiltration. These results indicate that ACSL4 expression is down-regulated and associated with prognosis in most tumors. In general, lower ACSL4 expression shows more beneficial prognosis. The most common genetic alteration of ACSL4 is point mutation. ACSL4 is negatively correlated with DNA methylation levels in most cancers. ACSL4 mutations or hypomethylation are associated with poor prognosis. In addition, ACSL4 is positively correlated with immune infiltration in cancers. ACSL4 and immune infiltration are strongly associated with prognosis in BRCA (Breast invasive carcinoma) and SKCM (Skin Cutaneous Melanoma). ACSL4 mutation caused significant changes of immune infiltration in UCEC (Uterine Corpus Endometrial Carcinoma) and SARC (Sarcoma). ACSL4 may be a promising prognostic biomarker for pan-cancer and is closely associated with immune infiltration in the tumor microenvironment.

目的 通过对炎症性肠病差异基因的筛选以及结直肠癌队列生存特征和表达模式的探究,为炎症相关结直肠癌的发生与发展的后续研究提供候选基因.方法 从GEO数据库中选择RNA测序表达谱数据集GSE95473和GSE107597,通过常规转录组表达谱差异分析,筛选出炎症性肠病差异表达基因(DEG),利用GO数据库获取DEG的功能注释,并利用KEGG数据库进行通路富集分析.同时基于TCGA数据库进一步在结直肠癌数据集中筛选具有预后意义的基因,并评价其在结直肠肿瘤中的表达特征.结果 两个数据集筛选到了共有DEGs 100个,通过主成分分析证实这些基因能够对结直肠癌肿瘤和黏膜区分良好.进一步筛选,获得ALDOB,SPINK4,REG4,IL1B,C2CD4A,CXCL8,NOS2,CXCL3等候选基因.这些基因在结直肠肿瘤中高表达,并且这些基因的高表达往往提示患者预后较好.结论 ALDOB,SPINK4,REG4,IL1B,C2CD4A,CXCL8,NOS2,CXCL3可能在炎症相关结直肠癌的发生发展中发挥重要作用,有待于后续炎癌转化相关功能验证和机制探究.

目的:分析并讨论腹腔镜手术与开放式修补术应用于老年胃溃疡穿孔患者中的治疗效果,及对患者生活质量和并发症发生情况的影响.方法:本组课题的受试对象为70例老年胃溃疡穿孔患者,遴选时间为2018年1月 ～2019年12月.按照随机数表法的分配原则,将所选的70例受试对象均分为两组.予以常规组的35例患者开放式修补术治疗,予以治疗组的35例患者腹腔镜手术治疗.根据两组患者的治疗情况,评估其生活质量,并记录并发症情况.结果:治疗组生活质量评分显著高于常规组,P<0.05;治疗组术后并发症发生率显著低于常规组,P<0.05.结论:应用腹腔镜手术治疗老年胃溃疡穿孔,能够显著降低并发症发生率,提升患者的生活质量,进而获得十分肯定的治疗效果,值得在临床上深入研究或推广.

Objective To investigate the clinical significance of plasma circRNA in colorectal cancer patients. Methods All the plasma were collected from colorectal cancer patients and healthy population, and the differently expressed circRNA were detected by Array and PCR. Then, we explored the correlation between circRNA and pathological indicators and patients′ prognosis. Results circPRMT5 was highly expressed in colorectal cancer patients. The expression of circPRMT5 had a correlation with the late stage, lymph node metastasis and prognosis. We also found the higher expression of circPRMT5 predicted a poor prognosis. Through univariate and multivariate analysis, we found circPRMT5 was an independent factor to predict the colorectal cancer patients prognosis. Conclusion circPRMT5 is a novel plasma molecular with clinical significance. Key words: Colorectal neoplasms; circPRMT5; Prognosis; Lymph node metastasis