目的:对比研究国产与进口两种ePTFE的生物相容性及其与角膜的生物愈合特性,寻找国产替代材料,观察植入物对角膜代谢的影响.方法:将孔径50μm、孔隙率88%、厚200μm的进口ePTFE与孔径45μm、孔隙率84%、厚2∞μm的国产ePTFE制成8mm直径圆盘植入兔角膜板层中,裂隙灯观察并照相,术后不同时间点分别处死动物进行光镜、透射电镜检查.结果:角膜成纤维细胞能长入ePTFE的微孔内并合并成胶原,各时间点二组植入物网孔内成纤维细胞数与胶原含量相同;最长观察期7个月植入物稳定;进口与国产两组分别有6眼与3眼(p>0.05)发生角膜脂肪变性.结论:ePTFE具有良好的生物相容性,能与宿主角膜产生生物愈合,植入ePTFE未造成严重的角膜营养代谢障碍.国产ePTFE具有与参数基本相近的进口ePTFE同样良好的生物相容性及与角膜生物愈合特性,国产材料的弹性较进口材料好.国产孔径45μm、孔隙率84%,厚200μm的ePTFE可用于制作人工角膜的周边部.

PURPOSE To evaluate the biocompatibility and 'healing' of Bioglass Ceramic(BGC) in the cornea. METHODS BGC dicks (8 mm in diameter, 0.5 mm thick, 20-70 microns pore diameter, 37-62% porosity, 7.8 mm radius of curvature) were implanted in 11 New Zealand albino rabbits after a free-hand intralamellar dissection. The eyes were examined by slit-lamp biomicroscope up to seven months postoperatively. The corneas were removed at seven month (one eye at eight month), then were examined by light microscope. RESULTS Five of 11 dicks of BGC were extruded. All implant of BGC with 51-62% porosity were broken and then extruded. A large part of implants led to markedly edematous with severe degrees of corneal neovascularization within one month postoperatively. The corneal lamella before implants became not clear in all eyes after two months. Lipid deposits occurred in four eyes. It is difficulty to observe the 'healing' process of BGC in the cornea because the BGC is too hard to be cut in spite of being soft by 4% hydrochloric acid for four to ten days before cutting. CONCLUSION Our findings suggest that BGC with above parameters is not suitable as peripheral keratoprosthetic materials because of its rough, hard, thick and breakable.

Purpose: To evaluate the biocompatibility and 'healing' process of gold with holes in the cornea. Methods: 9.999K gold dick (8mm in diameter, 0.1mm in thickness, with about 80 holes, 7.8mm radius of curvature) were implanted in 17 New Zealand albino rabbits after a free-hand intralamellar dissection. The eyes were examined by slit-lamp biomicroscopy up to 7 months postoperatively. The corneas were removed and examined by light microscope, SEM and TEM. Result: Dicks remained in corneas for periods of up to 7 months without any extrusion. Some of implants led to markedly edematous with varying degrees of cornear neovascularization within 1 month postoperatively. Stromal fibroblasts penetrate, proliferate, and synthesize collagen in the holes of gold plate. Calcification was found in 8 eyes. No capsule was formed around the implants. Conclusion: Our findings suggest that 9.999K gold dick with a number of holes may be successful as peripheral keratoprosthetic material which is able to provide a permanent and tight fusion of the keratoprosthesis with the host tissue. It is necessary to increase number of holes for preventing calcification in the cornea.

目的:探讨国产珊瑚羟基磷灰石(HA)眶内植入物的生物相容性以及植入兔眶内后纤维血管化情况.方法:在18只兔眶内植入国产珊瑚HA,于术后定期取出植入物作光镜与透射电镜检查.结果:18例中有2例出现植入物暴露,无发生植入物暴露的组织学改变显示:术后6周时植入物已全部纤维血管化,仅有少许炎症细胞;发生植人物暴露的组织学改变显示:植入物外围少许纤维血管长进,内围大量炎症细胞浸润.结论:①国产珊瑚HA具有良好的生物相容性,植入兔眶内后6周可达到完全纤维血管化.②植人物暴露的发生与纤维血管延迟内生和HA引起的炎症反应有关.