Objective To explore the phenolic tetrahydro-β-carboline-arginine-glycine-aspartic acid (THBCB-RGD) peptidomimetic conjugates' role in myocardial ischemia-reperfusion injury.Methods Using high performance liquid chromatography (HPLC) method to detect the stability of THBCB-RGD peptidomimetic conjugates.The oxyradical scavenging ability of THBCB-RGD peptidomimetic conjugates was assessed by PC12 cells survive experiment and acetylcholine induced vasodilatation experiment assessment.The antiplatelet aggregation activity of THBCB-RGD peptidomimetic conjugates was evaluated by platelet aggregation exprement.Through the in vivo experiment,we evaluated the antithrombotic activity of THBCB-RGD peptidomimetic conjugates.Next,we constructed the cardiac ischemia reperfusion model and observe the effects of phenolic THBCB-RGD peptidomimetic conjugates on myocardial oxidative stress and myocardial infarction.Results HPLC results show that THBCB-RGD peptidomimetic conjugates has a strong ability of scavenging (exhaustion time:240 min,180 min,240 min;RHBCB:30 min;P =0.005).NO,H2O2 and · OH as well as inhibiting platelet aggregation and thrombus formation (P =0.004 or P =0.000).It can also inhibit the aggregation of platelets and the formation of thrombus (P =0.003 or P =0.000).In addition,treatment with phenolic tetrahydro-β-carboline-RGD peptidomimetic conjugates before the heart ischemia-reperfusion injury can decrease the MDA content in myocardium and reduce the myocardial infarction area.Conclusion THBCB-RGD peptidomimetic conjugates showed a strong ability of scavenging oxyradical and anti-thrombosis.Addition,THBCB-RGD peptidomimetic conjugates play a protective role in cardiac ischemia-reperfusion injury by inhibiting oxidative stress and reducing the myocardial infarction area.

目的 探讨在下肢深静脉血栓形成(deep venous thrombosis,DVT)介入治疗过程中腔静脉滤器应用的必要性和安全性.方法 回顾性分析2015年8月至2016年8月河北医科大学第二医院血管外科收治的58例急性DVT患者,均接受导管接触溶栓(catheter directed thrombolysis,CDT)治疗,根据髂静脉受压情况分为2组,即置入下腔静脉滤器(inferior vena cava filter,IVCF)组39例和非IVCF组19例.记录2组患者术中、术后发生肺栓塞(pulmonary embolism,PE)的情况和因为手术而产生的医疗费用;记录因为置入IVCF而引发的并发症.结果 58例DVT患者均成功接受CDT治疗,未发生PE.非IVCF组平均手术费用比IVCF组减少了3.9万元.在IVCF组中2例患者IVCF下方继发血栓堵塞下腔静脉,2例患者因为IVCF与下腔静脉内膜过度粘连而未能取出,1例患者因为IVCF变形而部分穿出下腔静脉.结论 对于髂静脉重度狭窄甚至闭塞的DVT患者,在接受CDT治疗时不需要置入IVCF,这样可以有效地减少医疗费用和因为IVCF置入而导致的并发症的发生率.

Objective To observe the changes of lung injury caused by different ischemia time of skeletal muscle . Methods Twenty-four male Wistar rats were randomly assigned for four groups , group I as the control group , only general anesthesia , did not block the hind limb blood flow;group II for blocking hind limb blood flow for 2 h and reperfusion for 6 h;group III for blocking hind limb blood flow 4 h and reperfusion 6 h;group IV for ischemia 8h and 6 h of reperfusion;detected rat’s serum lactate dehydrogenase (LDH) and malondialdehyde (MDA) content, muscle tissue and lung tissue pathological changes.Results (1) LDH levels:after the experiment , changes in group I is not obvious , group II, III and IV showed a rising trend, the difference were significant compared with group I ( P <0.05), and the group IV >group III >group II (P <0.05).(2) The level of MDA:after the experiment, group I and group II changes were not obvious , and group III and IV showed a rising trend, and group IV >group III >group II and I ( P <0.05).(3) The microscopic findings of the muscle tissue were increased with the increase of ischemia time , and the damage of the IV group was severe .But lung tissue injury only found in group III and IV , and gradually increased the level of blocking time .Conclusion Rat skeletal muscle is-chemia and after reperfusion tissue damage in accordance with the prolongation of ischemia time and aggravating , ischemia for 4 h and reperfusion 6 h can occurred with lung injury .