A novel super compact mutant, scp-3, was identified using map-based cloning in cucumber. The CsDWF7 gene encoding a delta7 sterol C-5(6) desaturase was the candidate gene of scp-3. Mining dwarf genes is important in understanding stem growth in crops. However, only a small number of dwarf genes have been cloned or characterized. Here, we characterized a cucumber (Cucumis sativus L.) dwarf mutant, super compact 3 (scp-3), which displays shortened internodes and dark green leaves with a wrinkled appearance. The photosynthetic rate of scp-3 is significantly lower than that of the wild type. The dwarf phenotype of scp-3 mutant can be partially rescued by the exogenous brassinolide (BL) application, and the endogenous brassinosteroids (BRs) levels in the scp-3 mutant were significantly lower compared to the wild type. Microscopic examination revealed that the reduced internode length in scp-3 resulted from a decrease in cell size. Genetic analysis showed that the dwarf phenotype of scp-3 was controlled by a single recessive gene. Combined with bulked segregant analysis and map-based cloning strategy, we delimited scp-3 locus into an 82.5 kb region harboring five putative genes, but only one non-synonymous mutation (A to T) was discovered between the mutant and its wild type in this region. This mutation occurred within the second exon of the CsGy4G017510 gene, leading to an amino acid alteration from Leu156 to His156. This gene encodes the CsDWF7 protein, an analog of the Arabidopsis DWF7 protein, which is known to be involved in the biosynthesis of BRs. The CsDWF7 protein was targeted to the cell membrane. In comparison to the wild type, scp-3 exhibited reduced CsDWF7 expression in different tissues. These findings imply that CsDWF7 is essential for both BR biosynthesis as well as growth and development of cucumber plants.

Tillage complicates erosion on sloping fields, especially in regions of combined wind and water erosion. The wind-water erosion crisscross region of the Chinese Loess Plateau is an example of such a region wherein erosion is caused by the stimulating effects of tillage, rainfall, and wind. Sheet erosion is an important form of cropland soil erosion and is closely correlated to the productive capacity of land. It is important to better understand the influence of tillage on soil sheet erosion in order to estimate the loss of fertile surface soil and to provide suitable tillage recommendations for the cultivation of sloping cropland in this region, which is susceptible to multiple erosion factors. This study aims to clarify spatial distribution and variation of sheet erosion rates as affected by different tillage patterns on sloping cropland under different slope conditions. We applied beryllium-7 measurements and sediment collection methods to estimate sheet erosion rates to reveal the distribution of sheet erosion on bare sloping fields, to determine correlations between sheet erosion rates and slopes (0, 5, 10, 15, 20, and 25), and to analyze variation in sheet erosion affected by tillage patterns on a plot scale. Results show that sheet erosion increased in a top-down direction and achieved maximum values at the mid- or lower-mid section of slopes, then erosion rates decreased or sediment deposition occurred at the base of slopes. Sheet erosion intensified with an increase in slope gradient fitting to linear or exponential functions regardless of the tillage pattern used (i.e., whether solely applying autumn plowing or a combination of autumn and spring plowing). However, tillage patterns had an obvious effect on sheet erosion. Differences in sheet erosion caused by tillage patterns increased from 5 to between 15 and 20 before decreasing at 25. This demonstrates that steep slope gradients will weaken tillage pattern effects on sheet erosion, thus becoming a significant impacting factor on sheet erosion. Based on sheet erosion rates, we recommend that cropland be converted to grassland or shrubland when slope gradients are greater than 20 in the study region. Notably, a decrease in tillage intensity (frequency) helped in the reduction of sheet erosion on sloping croplands, and further tillage practices associated with conservation tillage should be applied on sloping cropland to obtain better soil erosion control results.

Soil erosion is complex in the wind-water erosion crisscross region of the Chinese Loess Plateau, as interleaving of wind and water erosion occurs on both temporal and spatial scales. It is difficult to distinguish wind erosion from the total erosion in previous studies due to the untraceable of aeolian particles and the limitation of feasible methods and techniques. This study used beryllium-7 measurements to study wind erosion in the wind-water erosion crisscross region on the Chinese Loess Plateau arms to delineate wind erosion distribution, to analyze its implication to erosive winds and surface microrelief, and to determine correlations between erosion rates and slope gradients. Results obtained using beryllium-7 measurements based on observation plots were verified with saltating particle collection method, and were also verified on a field scale. Results indicated that the effective resultant erosion wind was from northward, which was proved by the eight-directional distributed saltating particles. The microrelief of the ground surface contributed to the formation of high or low erosion centers. Wind erosion rates increased with a linear (R2≥0.95) or exponential (R2≥0.83) fitting increase in the slope gradients as reported in previous studies. Compared to wind erosion on field scale, both the plots and fields exhibited similar distribution patterns in wind erosion isolines. We also determined that the wind erosion rate for two fields estimated, based on equations developed from plot scale was acceptable. This study validates the feasibility of beryllium-7 measurements for soil-wind erosion field experiments and the potential to expand this approach to real field conditions.

Elastic laminae are extracellular matrix constituents that not only contribute to the stability and elasticity of arteries but also play a role in regulating arterial morphogenesis and pathogenesis. We demonstrate here that an important function of arterial elastic laminae is to prevent monocyte adhesion, which is mediated by the inhibitory receptor signal regulatory protein (SIRP) alpha and Src homology 2 domain-containing protein-tyrosine phosphatase (SHP)-1. In a matrix-based arterial reconstruction model in vivo, elastic laminae were resistant to leukocyte adhesion and transmigration compared with the collagen-dominant arterial adventitia. The density of leukocytes within the elastic lamina-dominant media was about 58-70-fold lower than that within the adventitia from 1 to 30 days. An in vitro assay confirmed the inhibitory effect of elastic laminae on monocyte adhesion. The exposure of monocytes to elastic laminae induced activation of SIRP alpha, which in turn activated SHP-1. Elastic lamina degradation peptides extracted from arterial specimens could also activate SIRP alpha and SHP-1. The knockdown of SIRP alpha and SHP-1 by specific small interfering RNA diminished the inhibitory effect of elastic laminae, resulting in a significant increase in monocyte adhesion. These observations suggest that SIRP alpha and SHP-1 potentially mediate the inhibitory effect of elastic laminae on monocyte adhesion.