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The research in my laboratory involves investigating the structure and mechanism of clathrin coated vesicle formation during clathrin-mediated endocytosis using a range of structural and biophysical techniques.
Receptor-mediated endocytosis allows a cell to choose and absorb the substances it requires. During endocytosis a clathrin-coated vesicle is formed which contains only specifically selected cargo. The clathrin coat is central to the ability of the vesicle to choose its contents and we aim, through a combination of structural and biophysical analysis to determine the factors that control its formation and disassembly
We are currently investigating how the molecular chaperone, Hsc70, and its cofactor auxilin, cause clathrin cage disassembly in vitro. We are using two approaches to investigate this. In the first we use 3D cryo-electron microscopy techniques to analyse the structure of purified clathrin cages bound to a range of proteins including Hsc70 and auxilin. In the second approach we monitor the kinetics of the process of clathrin cage disassembly using a range of biophysical techniques such as fluorescence anisotropy, stopped flow kinetics, isothermal titrating calorimetry and light scattering. We are now broadening our studies to investigate the part played by adaptor proteins and nucleotide exchange factors in the formation and disassembly of clathrin cage complexes.
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Biophysical journalno. 3S1 (2023): 514a-514A
Saskia E Bakker,David Bhella, Rosaria Brescia,Per Bullough,Daniel K Clare,Bertram Daum,René A W Frank,Vicki A M Gold, Isobel Jackson Hirst,Werner Kühlbrandt,Penghan Lu,Mathew McLaren,
Nature communicationsno. 1 (2020): 3252-3252
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