基本信息
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职业迁徙
个人简介
LABORATORY SKILLS
Experiences in culturing of embryonic neurons (Primary cultures, Cell lines)
Animal studies In utero Electroporation to check knockdown and overexpression of target gene and neonatal cortical primary neuron isolation.
Intensive experience in neuronal differentiation.
Deep sequencing of protein-bound RNA (RIP seq). RIP seq is used to screen the downstream target of a specific phenotype causing RNA binding protein.
Experiences in the design of vectors and cloning of the vectors.
Experiences in designing lentiviral vector and lentivirus particle production and titration and transduction of lentivirus particles in neurons and other cell lines.
Immunocytochemistry (Confocal microscopy, phase contrast microscopy)
RT-PCR, Real-time PCR
RNA half-life assay
Western bolting, IP, FACS analysis
Experiences in RNA competition assay and biotinylated protein-RNA pull-down assay
Experiences in Boyden chamber transwell migration assay both for neuron and cancer cells to check the in vitro migration of cells.
Experiences in proliferation (BrdU) assay.
Research interest
Stroke
Vascular contributions to cognitive impairment and dementia (VCID)
Cellular and Molecular Neuroscience
Developmental neuroscience and diseases.
Functional Genomics in rare and neurological diseases
RNA Biology in Drug Discovery of neuroscience and Cancer.
Cancer immunology
Organoid-based regenerative medicine (Brain organoid)
Cell fate change or tissue engineering for future regenerative medicine in neuroscience and cancer
研究兴趣
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semanticscholar(2016)
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D-Core
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