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个人简介
In our laboratory, we are particularly interested in understanding how genetic alterations affect the 3D structures of proteins, and deregulate their associated signalling pathways. Our experimental tools include NMR spectroscopy, X-ray crystallography, cryo-EM, fluorescence resonance energy transfer (FRET) and total internal reflection fluorescence (TIRF) imaging microscopy. NMR, X-ray, and cryo-EM enable us to determine atomic-resolution 3D structures of key molecular players and analyze the dynamics and kinetics of their interactions, while in-cell FRET and TIRF microscopy let us visualize where and how the signalling molecules work in the cell in response to a stimulus. We combine structural and biochemical analyses with functional studies on mammalian cell lines and model organisms using advanced technologies such as gene knock-out and RNAi-based gene silencing. Presently our research focuses on signalling pathways involved in three key aspects of cancer: i) small GTPase signalling, ii) cell adhesion and polarity, and iii) calcium homeostasis. We believe that our molecular studies of cancer cell signalling will contribute to a deeper understanding of this life-threatening human disease.
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Takashi Saitoh, Ha-Neul Kim, Riku Narita, Ibuki Ohtsuka, Weiyu Mo,Ki-Young Lee,Masahiro Enomoto,Geneviève M C Gasmi-Seabrook,Christopher B Marshall,Mitsuhiko Ikura
Protein expression and purification (2023): 106361
Yuou Wang,Alex Yemelyanov, Christopher D Go,Sun Kim,Jeanne M Quinn,Annette S Flozak, Phuong M Le, Shannon Liang,Anne Claude-Gingras,Mitsu Ikura,Noboru Ishiyama,Cara J Gottardi
bioRxiv : the preprint server for biology (2023)
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