基本信息
浏览量:1
![](https://originalfileserver.aminer.cn/sys/aminer/icon/show-trajectory.png)
个人简介
Between March 2001 and December 2014, I was part of the Center of Experimental Morphology (CME - FCT Unit 121/94), where I participated in the research projects under POCTI/NSE/35767/99 and POCTI/NSE/42834/2001.
During this period, I have learned and mastered various laboratory and histological techniques necessary for neurobiological research. Of these, I highlight the perfusion and removal of rat brains, material processing for embedding in epoxy resin and in glycolmethacrylate and Golgi impregnation methods; cutting brains in Vibratome and Cryostat; immunocytochemical techniques by the method of avidin-biotin and fluorescence; in situ hybridization techniques; techniques for processing and slicing material for electron microscopy, handling electron microscope, developing and printing photographs of electron microscopy. In order to conduct quantitative studies, I have learned and applied the stereological methods necessary to estimate volumes of brain areas/nuclei, cell volumes, total numbers of cells and synapses, surface area determinations and numerical density of particles. I have developed and applied methods for quantification by densitometry, in raw material processed for in situ hybridization or immunofluorescence. The studies I have accomplished were conducted in several areas of the central nervous system, including the hippocampal formation, the magnocellular basal nucleus, the medial pre-optic nucleus and hypothalamic suprachiasmatic and ventromedial nuclei.
From January 2015, I am an Investigator of the CINTESIS - Center for Health Technology and Services Research, located at the Faculty of Medicine, University of Porto.
From September 2012, I was the MSc theses advisor of two students from Faculty of Medicine, University of Porto, the MSc theses co-advisor of one student from Faculty of Pharmacy, University of Porto and of two students from University of São Paulo, Brazil, and the PhD advisor of one student from Faculty of Medicine, University of Porto.
In the present, my studies aim at understanding the ovarian hormone-induced neuronal mechanisms and circuitries that set in motion hormone-dependent cognition and behavior. This subject is of major importance because hormonal therapy using pharmacological agents and selective estrogen receptor modulators, offered to promote birth control, to prevent estrogen-related forms of cancer and to lessen menopause effects is increasing, without the knowledge of the effects of hormone receptor modulation in the female brain. By using female rat models, my study focus in the action of hormone receptors and the consequences of its activation/blockage in the modulation and triggering of neuronal networks leading to the construction of a behavioral response.
研究兴趣
论文共 46 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
Archives of Toxicologyno. 1 (2024): 95-119
Sara Marques,Vladimir D. Stevanović, J. Aranđelović,Miroslav M. Savić,Helena Carmo,F. Carvalho,Susana I. Sá,João Pedro Silva
Toxicology Letters (2023): S66-S66
crossref(2023)
Elsevier eBookspp.541-553, (2023)
引用0浏览0引用
0
0
International Journal of Molecular Sciencesno. 17 (2023): 13126-13126
加载更多
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn