基本信息
浏览量:79
职业迁徙
个人简介
My research interests include both prokaryotic and eukaryotic molecular biology studies that relate to human health and diseases. Earlier in my career in prokaryotic molecular biology, I conducted research in a specific area of food microbiology for isolating, purifying, and discovering a plasmid (pSMB74) and its curing as well as conjugal transfer for linking its ability for production of a bacteriocin and immunity in a strain of Pediococcus acidilactici. Then, I moved to eukaryotic molecular biology to work as a Postdoctoral Fellow with the world-renowned late Biochemist Betsy M. Sutherland, PhD, and conducted research on mechanisms of ultraviolet (UV) light induced DNA damage and repair in human skin fibroblasts and human skin tissues at Brookhaven National Laboratory (Upton, NY) in collaboration with a group of Photomedicine Laboratory at Harvard Medical School (Boston, MA). Subsequently, I took an interest in the mechanisms of induction of apoptosis, also known as programmed cell death, in human leukemia and breast cancer cells after treatments with standard and experimental therapeutic agents at Medical University of South Carolina (Charleston, SC) and Winship Cancer Center at Emory University (Atlanta, GA). I developed a sensitive method for isolation and detection of internucleosomal DNA fragmentation (a hallmark of apoptosis) in low number of human acute myeloid leukemia cells following therapeutic treatments, discovered that transfection of the human pro-apoptotic Bcl-xS gene using a mammalian expression plasmid vector induced erythrocytic differentiation and enhanced efficacy of differentiating agents in human chronic myeloid leukemia cells, invented an in situ method for simultaneous detection of apoptotic DNA fragmentation in a specific cell type in tissue section, created both non-radioactive and radioactive versions of the polymerase chain reaction (PCR) based telomerase assay for increasing its sensitivity and processivity, established for the first time that the Ca2+-dependent cysteine proteases in addition to the Ca2+-independent cysteine proteases participated in induction of apoptosis in the central nervous system (CNS) cells, proved preservation of electrophysiological functions of the neurons in vitro and recovery of neurological functions in vivo following inhibition of cysteine proteases, and serendipitously for the first time found pathogenesis in the spinal cord in addition to brain in animal model of Parkinson’s disease and involvement of calpain in this process. Ever since I started my graduate studies, I had an insatiable curiosity about the structure and functions of the human brain (arguably the most complex thing in our known universe), workings in the whole CNS, and mechanisms of pathogenesis in CNS disorders. I had a dream of getting involved in research to find out the targeted treatments for the CNS disorders. My dream came true as I started my career as a junior faculty, and it continued forever for the challenging CNS studies.
研究兴趣
论文共 280 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
Methods in molecular biology (Clifton, N.J.) (2024): 149-157
Methods in molecular biology (Clifton, N.J.) (2024): 1-26
Ahalya Muraleedharan,Swapan K. Ray
BRAIN SCIENCESno. 1 (2024): 96
Cellsno. 6 (2023): 897-897
Cellular, Molecular, Physiological, and Behavioral Aspects of Spinal Cord Injurypp.263-274, (2022)
CELLSno. 9 (2021): 2342
加载更多
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn