A streamlined workflow for automated cryo focused ion beam milling

Sebastian Tacke,Philipp Erdmann,Zhexin Wang,Sven Klumpe,Michael Grange,Radim Kříž,Matej Dolník,John Mitchels,Jürgen Plitzko,Stefan Raunser

Journal of Structural Biology（2021）

引用 41|浏览28

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摘要

•Automated high-quality, high-throughput cryo-FIB milling.•Glove box and a cryo transfer system for optimal sample preparation and transfer.•Stage heater, Cryo-Shield and Cryo-Shutter for efficient FIB milling.•Optimal conditions for high-resolution structural biology of the cell.

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关键词

Electron tomography,Focused ion beam,Cryo shield,Cryo shutter,Contamination,Automation

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